Matches in SemOpenAlex for { <https://semopenalex.org/work/W2036745314> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2036745314 endingPage "303" @default.
- W2036745314 startingPage "292" @default.
- W2036745314 abstract "Allergy results from inappropriate immune responses to normally innocuous proteins. More than 300 IgE-binding proteins have been cloned and shown to cover structurally and functionally heterogeneous protein families including enzymes, backbone and storage proteins as well as proteins with unknown function. Structurally related cross-reactive allergens are involved in the pathogenesis of important clinical syndromes. We found that phylogenetically highly conserved proteins, including human manganese superoxide dismutase (MnSOD), are involved in IgE-mediated autoreactivity.MnSOD cloned from different phylogenetically distant species were produced as recombinant proteins in Escherichia coli and used to study IgE-mediated cross-reactivity and proliferative responses in peripheral blood mononuclear cells (PBMC) of individuals sensitised to Aspergillus fumigatus MnSOD. Homology models of the three-dimensional structures of MnSOD from A. fumigatus, Drosophila melanogaster and Saccharomyces cerevisiae using the human MnSOD structure refined at 2.2 A as template were constructed to identify conserved amino acid residues exposed to the solvent.Cross-reactivity between the MnSOD at B and T cell level was demonstrated by inhibition experiments showing shared B cell epitopes and by the capability of the different MnSODs to induce proliferative responses in PBMCs of sensitised individuals. Structural modelling allowed to identify conserved residues exposed to the solvent. The identified residues are scattered over the sequence of the enzyme indicating putative conformational IgE-binding epitopes.The results obtained corroborate molecular mimicry as a plausible mechanism to explain autoreactivity to human MnSOD. Moreover, homology modelling provides a rational tool to identify conserved residues involved in defining cross-reactive B cell epitopes." @default.
- W2036745314 created "2016-06-24" @default.
- W2036745314 creator A5024419542 @default.
- W2036745314 creator A5025119080 @default.
- W2036745314 creator A5029018809 @default.
- W2036745314 creator A5043404030 @default.
- W2036745314 creator A5068882554 @default.
- W2036745314 creator A5085722194 @default.
- W2036745314 date "2002-01-01" @default.
- W2036745314 modified "2023-09-27" @default.
- W2036745314 title "Immunological and Structural Analysis of IgE-Mediated Cross-Reactivity between Manganese Superoxide Dismutases" @default.
- W2036745314 cites W1976499671 @default.
- W2036745314 cites W1986191025 @default.
- W2036745314 cites W2003113302 @default.
- W2036745314 cites W2010143299 @default.
- W2036745314 cites W2018654176 @default.
- W2036745314 cites W2041136773 @default.
- W2036745314 cites W2069333165 @default.
- W2036745314 cites W2104042418 @default.
- W2036745314 cites W2157880891 @default.
- W2036745314 cites W4243207870 @default.
- W2036745314 cites W4243459578 @default.
- W2036745314 cites W4250822001 @default.
- W2036745314 doi "https://doi.org/10.1159/000063862" @default.
- W2036745314 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12218367" @default.
- W2036745314 hasPublicationYear "2002" @default.
- W2036745314 type Work @default.
- W2036745314 sameAs 2036745314 @default.
- W2036745314 citedByCount "57" @default.
- W2036745314 countsByYear W20367453142012 @default.
- W2036745314 countsByYear W20367453142013 @default.
- W2036745314 countsByYear W20367453142014 @default.
- W2036745314 countsByYear W20367453142015 @default.
- W2036745314 countsByYear W20367453142017 @default.
- W2036745314 countsByYear W20367453142018 @default.
- W2036745314 countsByYear W20367453142019 @default.
- W2036745314 countsByYear W20367453142021 @default.
- W2036745314 countsByYear W20367453142022 @default.
- W2036745314 countsByYear W20367453142023 @default.
- W2036745314 crossrefType "journal-article" @default.
- W2036745314 hasAuthorship W2036745314A5024419542 @default.
- W2036745314 hasAuthorship W2036745314A5025119080 @default.
- W2036745314 hasAuthorship W2036745314A5029018809 @default.
- W2036745314 hasAuthorship W2036745314A5043404030 @default.
- W2036745314 hasAuthorship W2036745314A5068882554 @default.
- W2036745314 hasAuthorship W2036745314A5085722194 @default.
- W2036745314 hasConcept C104317684 @default.
- W2036745314 hasConcept C141105273 @default.
- W2036745314 hasConcept C159654299 @default.
- W2036745314 hasConcept C167625842 @default.
- W2036745314 hasConcept C169627665 @default.
- W2036745314 hasConcept C181199279 @default.
- W2036745314 hasConcept C195616568 @default.
- W2036745314 hasConcept C203014093 @default.
- W2036745314 hasConcept C2777119779 @default.
- W2036745314 hasConcept C2779142602 @default.
- W2036745314 hasConcept C3020064264 @default.
- W2036745314 hasConcept C55493867 @default.
- W2036745314 hasConcept C86803240 @default.
- W2036745314 hasConcept C8891405 @default.
- W2036745314 hasConceptScore W2036745314C104317684 @default.
- W2036745314 hasConceptScore W2036745314C141105273 @default.
- W2036745314 hasConceptScore W2036745314C159654299 @default.
- W2036745314 hasConceptScore W2036745314C167625842 @default.
- W2036745314 hasConceptScore W2036745314C169627665 @default.
- W2036745314 hasConceptScore W2036745314C181199279 @default.
- W2036745314 hasConceptScore W2036745314C195616568 @default.
- W2036745314 hasConceptScore W2036745314C203014093 @default.
- W2036745314 hasConceptScore W2036745314C2777119779 @default.
- W2036745314 hasConceptScore W2036745314C2779142602 @default.
- W2036745314 hasConceptScore W2036745314C3020064264 @default.
- W2036745314 hasConceptScore W2036745314C55493867 @default.
- W2036745314 hasConceptScore W2036745314C86803240 @default.
- W2036745314 hasConceptScore W2036745314C8891405 @default.
- W2036745314 hasIssue "4" @default.
- W2036745314 hasLocation W20367453141 @default.
- W2036745314 hasLocation W20367453142 @default.
- W2036745314 hasOpenAccess W2036745314 @default.
- W2036745314 hasPrimaryLocation W20367453141 @default.
- W2036745314 hasRelatedWork W1257198636 @default.
- W2036745314 hasRelatedWork W1908365524 @default.
- W2036745314 hasRelatedWork W1988863520 @default.
- W2036745314 hasRelatedWork W2028271463 @default.
- W2036745314 hasRelatedWork W2051192721 @default.
- W2036745314 hasRelatedWork W2087982072 @default.
- W2036745314 hasRelatedWork W2092258950 @default.
- W2036745314 hasRelatedWork W3103275457 @default.
- W2036745314 hasRelatedWork W4246441064 @default.
- W2036745314 hasRelatedWork W4383736489 @default.
- W2036745314 hasVolume "128" @default.
- W2036745314 isParatext "false" @default.
- W2036745314 isRetracted "false" @default.
- W2036745314 magId "2036745314" @default.
- W2036745314 workType "article" @default.