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- W2036758603 abstract "The effects of protein synthesis inhibitors, cycloheximide and puromycin, on functional thionein mRNA levels have been examined to further delineate the regulation of thionein gene expression in Cd2+-resistant CHO sublines proficient in Cd2+- or Zn2+-stimulated synthesis of metallothioneins. Inhibition of protein synthesis at or shortly before the time of metal-mediated induction only slightly reduces the usual increase in thionein mRNA. Protein synthesis inhibition alone does not significantly increase thionein mRNA levels. However, addition of cycloheximide 4 h after Zn2+ induction increases thionein mRNA activity measured 8 h after induction by at least 40%, suggesting that protein synthesis is required for normal turnover of thionein mRNA." @default.
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- W2036758603 date "1980-03-01" @default.
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- W2036758603 title "Regulation of induced thionein gene expression in cultured mammalian cells: Effects of protein synthesis inhibition on translatable thionein mRNA levels in regulatory variants of the CHO cell" @default.
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- W2036758603 doi "https://doi.org/10.1016/0006-291x(80)91082-7" @default.
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