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- W2036761279 abstract "Pig thyroid slices bind and engulf large amounts of 125I—labeled thyroglobulin (Tg*). After incubation with Tg*, the slices were homogenized and submitted to differential centrifugation (C: cellular debris; N: nuclei; M: heavy mitochondria; L: light mitochondria; P: microsomes and S: supernatant). The supernatant contained 62.1% of the radioactivity (28.0% of which was in TCA—soluble form) and 25.0% sedimented with the M + L particulate pellet together with the lysosomal enzymes. Equilibration isopycnic density gradient centrifugation of the M + L fraction showed an equilibration pattern of the radioactivity similar to that of acid hydrolases (modal density of 1.18–1.20). Increasing concentration of Triton X–100 (up to 0.1%) liberated conjointly M + L radioactivity and lysosomal enzymes. Sucrose density gradient centrifugation of the M + L soluble extract showed a large 3–8 S, a 12 S and a 19 S peak. In the supernatant, only 3–8 S and 19 S components were observed. T3 and T4 were present in the M + L fraction but were more abundant in the P + S fraction. The ratio T3/T4 approximated 1 in nearly all the samples. Iodide was mostly found in the supernatant where minute amounts of iodotyrosines were also detected. The iodoamino acid composition of the material liberated by intracellular proteolysis differed greatly from that obtained after hydrolysis of Tg* by pronase. These biochemical data are in agreement with the concept that Tg* taken up by thyroid slices is degraded inside the phagolysosomes. Dissociation of the Tg molecule into subunits seems to be necessary for catheptic proteolysis. Equal amounts of free T3 and free T4 are liberated from their peptide links while DIT and MIT are nearly absent. These results emphasize the specificity of the hydrolytic process and the dehalogenase present in the thyroid follicular cell. (Endocrinology91: 362, 1972)" @default.
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- W2036761279 date "1972-08-01" @default.
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- W2036761279 title "Subcellular Localization of Thyroglobulin Taken Up by Pig Thyroid Slices12" @default.
- W2036761279 doi "https://doi.org/10.1210/endo-91-2-362" @default.
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