FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2036769067> ?p ?o ?g. }

• W2036769067 endingPage "602" @default.
• W2036769067 startingPage "602" @default.
• W2036769067 abstract "Fluorescence microscopy offers a minimally perturbative approach to probe biology in vivo. However, available techniques are limited both in sensitivity and temporal resolution for commonly used fluorescent proteins. Here we present a new imaging system with a diagnostic toolkit that caters for the detection and quantification of fluorescent proteins for use in fast functional imaging at the single-molecule level. It utilizes customized microscopy with a mode of illumination we call slimfield suitable for rapid (approximately millisecond) temporal resolution on a range of common fluorescent proteins. Slimfield is cheap and simple, allowing excitation intensities approximately 100 times greater than those of widefield imaging, permitting single-molecule detection at high speed. We demonstrate its application on several purified fluorescent proteins in standard use as genetically-encoded reporter molecules. Controlled in vitro experiments indicate single protein molecules over a field of view of approximately 30 microm(2) area, large enough to encapsulate complete prokaryotic and small eukaryotic cells. Using a novel diagnostic toolkit we demonstrate automated detection and quantification of single molecules with maximum imaging rates for a 128 x 128 pixel array of approximately 500 frames per second with a localization precision for these photophysically poor fluorophores to within 50 nm. We report for the first time the imaging of the dim enhanced cyan fluorescent protein (ECFP) and CyPet at the single-molecule level. Applying modifications, we performed simultaneous dual-colour slimfield imaging for use in co-localization and FRET. We present preliminary in vivo imaging on bacterial cells and demonstrate approximately millisecond timescale functional imaging at the single-molecule level with negligible photodamage." @default.
• W2036769067 created "2016-06-24" @default.
• W2036769067 creator A5005495942 @default.
• W2036769067 creator A5064876331 @default.
• W2036769067 creator A5074311677 @default.
• W2036769067 date "2009-01-01" @default.
• W2036769067 modified "2023-09-26" @default.
• W2036769067 title "Millisecond timescale slimfield imaging and automated quantification of single fluorescent protein molecules for use in probing complex biological processes" @default.
• W2036769067 cites W1484258617 @default.
• W2036769067 cites W1542263802 @default.
• W2036769067 cites W1967386345 @default.
• W2036769067 cites W1984969423 @default.
• W2036769067 cites W1986752048 @default.
• W2036769067 cites W1988326309 @default.
• W2036769067 cites W1988949448 @default.
• W2036769067 cites W2006685379 @default.
• W2036769067 cites W2010181298 @default.
• W2036769067 cites W2012647985 @default.
• W2036769067 cites W2014293907 @default.
• W2036769067 cites W2017196830 @default.
• W2036769067 cites W2021748684 @default.
• W2036769067 cites W2023612132 @default.
• W2036769067 cites W2026078372 @default.
• W2036769067 cites W2027049904 @default.
• W2036769067 cites W2029225224 @default.
• W2036769067 cites W2032579724 @default.
• W2036769067 cites W2036964975 @default.
• W2036769067 cites W2037793351 @default.
• W2036769067 cites W2042050610 @default.
• W2036769067 cites W2051542254 @default.
• W2036769067 cites W2052512546 @default.
• W2036769067 cites W2054241952 @default.
• W2036769067 cites W2061464631 @default.
• W2036769067 cites W2069707833 @default.
• W2036769067 cites W2079531873 @default.
• W2036769067 cites W2081342248 @default.
• W2036769067 cites W2084312697 @default.
• W2036769067 cites W2086633179 @default.
• W2036769067 cites W2091723156 @default.
• W2036769067 cites W2096963388 @default.
• W2036769067 cites W2099247443 @default.
• W2036769067 cites W2100453710 @default.
• W2036769067 cites W2107236242 @default.
• W2036769067 cites W2115986304 @default.
• W2036769067 cites W2119344497 @default.
• W2036769067 cites W2121964182 @default.
• W2036769067 cites W2124778528 @default.
• W2036769067 cites W2127068865 @default.
• W2036769067 cites W2127628180 @default.
• W2036769067 cites W2132036867 @default.
• W2036769067 cites W2137055458 @default.
• W2036769067 cites W2160398772 @default.
• W2036769067 cites W2163977938 @default.
• W2036769067 cites W3044750868 @default.
• W2036769067 doi "https://doi.org/10.1039/b907837a" @default.
• W2036769067 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20023777" @default.
• W2036769067 hasPublicationYear "2009" @default.
• W2036769067 type Work @default.
• W2036769067 sameAs 2036769067 @default.
• W2036769067 citedByCount "101" @default.
• W2036769067 countsByYear W20367690672012 @default.
• W2036769067 countsByYear W20367690672013 @default.
• W2036769067 countsByYear W20367690672014 @default.
• W2036769067 countsByYear W20367690672015 @default.
• W2036769067 countsByYear W20367690672016 @default.
• W2036769067 countsByYear W20367690672017 @default.
• W2036769067 countsByYear W20367690672018 @default.
• W2036769067 countsByYear W20367690672019 @default.
• W2036769067 countsByYear W20367690672020 @default.
• W2036769067 countsByYear W20367690672021 @default.
• W2036769067 countsByYear W20367690672022 @default.
• W2036769067 countsByYear W20367690672023 @default.
• W2036769067 crossrefType "journal-article" @default.
• W2036769067 hasAuthorship W2036769067A5005495942 @default.
• W2036769067 hasAuthorship W2036769067A5064876331 @default.
• W2036769067 hasAuthorship W2036769067A5074311677 @default.
• W2036769067 hasConcept C104317684 @default.
• W2036769067 hasConcept C119666444 @default.
• W2036769067 hasConcept C120665830 @default.
• W2036769067 hasConcept C121332964 @default.
• W2036769067 hasConcept C122776584 @default.
• W2036769067 hasConcept C12554922 @default.
• W2036769067 hasConcept C1276947 @default.
• W2036769067 hasConcept C136339569 @default.
• W2036769067 hasConcept C138268822 @default.
• W2036769067 hasConcept C142613039 @default.
• W2036769067 hasConcept C147080431 @default.
• W2036769067 hasConcept C150903083 @default.
• W2036769067 hasConcept C154945302 @default.
• W2036769067 hasConcept C169274487 @default.
• W2036769067 hasConcept C185592680 @default.
• W2036769067 hasConcept C207001950 @default.
• W2036769067 hasConcept C2778067846 @default.
• W2036769067 hasConcept C29331040 @default.
• W2036769067 hasConcept C3019336641 @default.
• W2036769067 hasConcept C41008148 @default.
• W2036769067 hasConcept C55493867 @default.
• W2036769067 hasConcept C57477423 @default.

• next