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• W2036774410 abstract "The prokaryotic (70 S) monosome, composed of a roughly hemispherical 50 S large subunit and an elongate 30 S small subunit, appears in the electron micrograph in only a few common views representing the small number of preferred orientations assumed by the particle. Two of these, termed O and L views, have previously been characterized as the overlap and non-overlap projections; a third view, which we term the R view, represents the other endpoint of a rotational continuum with the overlap or O view. Tilt studies enabled us to calibrate this range as spanning ~50 °. The disjunct set of L views was averaged, and the reproducible resolution was determined to be 13.5 nm−1. The combined sets of O and R views were analyzed by correspondence analysis, and a continuous “rotation series” of subaverages was obtained. Interpretation of the views in the light of what is known about the morphologies of the individual subunits allows a general picture of the mutual fit of the subunits in the monosome to be conceived." @default.
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• W2036774410 date "1986-02-01" @default.
• W2036774410 modified "2023-09-26" @default.
• W2036774410 title "Computer-averaged views of the 70 S monosome from Escherichia coli" @default.
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• W2036774410 doi "https://doi.org/10.1016/0022-2836(86)90336-0" @default.
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