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• W2036870775 abstract "Comparisons of the relative activities of 11 intergenic region (IGR) internal ribosome entry site (IRES) elements of insect dicistrovirus with 5′ IRES elements of the hepatitis C and encephalomyocarditis viruses were performed in insect and mammalian cells. Dual luciferase assays were performed to determine the most effective dicistrovirus IGR IRES in the lepidopteran cell lines Sf9 ( Spodoptera frugiperda ) and BmN ( Bombyx mori ), and the dipteran cell lines S2 ( Drosophila melanogaster ) and ATC-10 ( Aedes aegypti ). Evaluation of dual luciferase expression from DNA plasmids and in vitro -transcribed RNA revealed apparent splicing with certain IRES elements. Though IRES activity depended upon the cell line examined, the black queen cell and Drosophila C dicistrovirus intergenic IRES elements were most effective for coupled gene expression in the diverse insect cell lines examined." @default.
• W2036870775 created "2016-06-24" @default.
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• W2036870775 date "2008-12-01" @default.
• W2036870775 modified "2023-09-23" @default.
• W2036870775 title "Examining the relative activity of several dicistrovirus intergenic internal ribosome entry site elements in uninfected insect and mammalian cell lines" @default.
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• W2036870775 doi "https://doi.org/10.1099/vir.0.2008/003921-0" @default.
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