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- W2036901278 abstract "The direct analysis of fluorescently labeled peptides by laser desorption/ionization-mass spectrometry (LDI-MS) is demonstrated for the first time. Peptides labeled on their N-termini with rhodamine B were analyzed by MS and tandem MS (MS/MS) using a recently developed visible-wavelength LDI-MS instrument. Labeling with a fluorescent dye leads to soft LDI of peptides in the absence of a matrix. The MS/MS spectra were simplified because only a and b rhodamine B-containing peptide fragment ions were observed, which allowed for easy spectral interpretation and de novo sequencing of peptides. In mixtures, the rhodamine B labeled peptides demonstrated a greater ionization efficiency, and therefore selectivity, compared to ionization of the unlabeled peptides. Additionally, FALDI-MS was used to directly analyze beta-amyloid peptides that were N-terminally labeled with the fluorophore HiLyte Fluor™ 555. The described FALDI-MS methodology provides a novel way to ionize and structurally characterize specifically labeled peptides." @default.
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- W2036901278 date "2013-11-01" @default.
- W2036901278 modified "2023-10-18" @default.
- W2036901278 title "Fluorophore-assisted laser desorption/ionization-mass spectrometry (FALDI-MS)" @default.
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- W2036901278 doi "https://doi.org/10.1016/j.ijms.2013.07.004" @default.
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