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- W2036980636 abstract "Many quantitative proteomics methods rely on protein and peptide labeling with stable isotopes. We have recently found that the introduction of ¹⁵N into organisms via in vivo metabolic labeling affects protein expression levels as well as metabolic pathways and behavioral phenotypes. Here, we present further evidence for a stable isotope effect based on the plasma proteome analysis of ¹⁵N-labeled mice. We compared plasma proteomes of ¹⁵N-labeled and unlabeled (¹⁴N) mice by quantitative MS. We found a number of protein level differences, some of which were verified immunochemically. In addition, we observed divergent chromatographic retention time and peak full width at half maximum (FWHM) between ¹⁵N-labeled and ¹⁴N tryptic peptides. Our data point toward a systemic effect of the introduction of heavy isotopes in vivo." @default.
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- W2036980636 date "2013-08-01" @default.
- W2036980636 modified "2023-10-16" @default.
- W2036980636 title "15N metabolic labeling: Evidence for a stable isotope effect on plasma protein levels and peptide chromatographic retention times" @default.
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- W2036980636 doi "https://doi.org/10.1016/j.jprot.2012.12.013" @default.
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