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- W2036996957 abstract "Abstract The relationship between the concentration of CoASH and the activity of serotonin N-acetyltransferase (NAT) was studied in rat pineal glands in culture. A technique for microdetermination of CoASH was developed by utilizing acetyl CoA synthetase and partially purified rat liver NAT. Initially CoASH was acetylated with [1–3H] acetate using acetyl CoA synthetase. Subsequently, the labelled acetyl group was transferred from [1–3H] acetyl CoA to tryptamine forming [1–3H acetyl-tryptamine which was then extracted into chloroform and measured by scintillation spectrometry. A direct relationship appeared to exist between the concentrations of CoASH and [1–3H] acetyltryptamine. This method is sensitive and specific since it can detect as low as 10–15 pmoles of CoASH but not structurally related substances such as acetyl CoA, ADP, cysteamine, or D-pantothenic acid. After treating the rat pineal glands in culture with 10 μM norepinephrine for six hours, the concentration of CoASH was found to decrease significantly from 31.96 ± 0.68 to 24.44 ± 0.37 pmoles/gland, while the activity of NAT increased 68 fold. This inverse relationship indicates that CoASH does not play a direct role in NAT induction although it does protect darktime NAT activity in pineal homogenates against thermal inactivation. The sensitivity and the adaptability of this method can be utilized to measure CoASH in discrete regions of rat brain and in experimental conditions where the micromeasurement of CoASH may be required." @default.
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- W2036996957 date "1981-02-01" @default.
- W2036996957 modified "2023-09-30" @default.
- W2036996957 title "A specific and sensitive enzymatic-isotopic microassay of coenzyme a in pineal gland" @default.
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- W2036996957 doi "https://doi.org/10.1016/0024-3205(81)90135-1" @default.
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