Matches in SemOpenAlex for { <https://semopenalex.org/work/W2037013112> ?p ?o ?g. }
- W2037013112 endingPage "923" @default.
- W2037013112 startingPage "916" @default.
- W2037013112 abstract "Molecular assays might improve the identification of causes of acute diarrheal disease but might lead to more frequent detection of asymptomatic infections. In the present study, real-time PCR targeting 14 pathogens was applied to rectal swabs from 330 children aged 2 to 59 months in Zanzibar, including 165 patients with acute diarrhea and 165 asymptomatic control subjects. At least one pathogen was detected for 94% of the patients and 84% of the controls, with higher rates among patients for norovirus genogroup II (20% versus 2.4%; P<0.0001), rotavirus (10% versus 1.8%; P=0.003), and Cryptosporidium (30% versus 11%; P<0.0001). Detection rates did not differ significantly for enterotoxigenic Escherichia coli (ETEC)-estA (33% versus 24%), ETEC-eltB (44% versus 46%), Shigella (35% versus 33%), and Campylobacter (35% versus 33%), but for these agents threshold cycle (CT) values were lower (pathogen loads were higher) in sick children than in controls. In a multivariate analysis, CT values for norovirus genogroup II, rotavirus, Cryptosporidium, ETEC-estA, and Shigella were independently associated with diarrhea. We conclude that this real-time PCR allows convenient detection of essentially all diarrheagenic agents and provides CT values that may be critical for the interpretation of results for pathogens with similar detection rates in patients and controls. The results indicate that the assessment of pathogen loads may improve the identification of agents causing gastroenteritis in children." @default.
- W2037013112 created "2016-06-24" @default.
- W2037013112 creator A5021808468 @default.
- W2037013112 creator A5033289218 @default.
- W2037013112 creator A5034131519 @default.
- W2037013112 creator A5048497439 @default.
- W2037013112 creator A5053546594 @default.
- W2037013112 creator A5063465178 @default.
- W2037013112 creator A5064345565 @default.
- W2037013112 creator A5076171817 @default.
- W2037013112 creator A5084119337 @default.
- W2037013112 date "2014-03-01" @default.
- W2037013112 modified "2023-10-03" @default.
- W2037013112 title "Real-Time PCR Threshold Cycle Cutoffs Help To Identify Agents Causing Acute Childhood Diarrhea in Zanzibar" @default.
- W2037013112 cites W157770237 @default.
- W2037013112 cites W1744198372 @default.
- W2037013112 cites W1848863761 @default.
- W2037013112 cites W1878888400 @default.
- W2037013112 cites W1928122503 @default.
- W2037013112 cites W1968938126 @default.
- W2037013112 cites W1971859359 @default.
- W2037013112 cites W1973266606 @default.
- W2037013112 cites W1981721009 @default.
- W2037013112 cites W1983892034 @default.
- W2037013112 cites W1987548420 @default.
- W2037013112 cites W1992727488 @default.
- W2037013112 cites W2012892833 @default.
- W2037013112 cites W2013479934 @default.
- W2037013112 cites W2016364046 @default.
- W2037013112 cites W2016890392 @default.
- W2037013112 cites W2039113258 @default.
- W2037013112 cites W2055548630 @default.
- W2037013112 cites W2070963274 @default.
- W2037013112 cites W2074029700 @default.
- W2037013112 cites W2085996589 @default.
- W2037013112 cites W2088976222 @default.
- W2037013112 cites W2091348396 @default.
- W2037013112 cites W2097300280 @default.
- W2037013112 cites W2097701592 @default.
- W2037013112 cites W2102833327 @default.
- W2037013112 cites W2106303448 @default.
- W2037013112 cites W2110641762 @default.
- W2037013112 cites W2114053580 @default.
- W2037013112 cites W2114581826 @default.
- W2037013112 cites W2115573931 @default.
- W2037013112 cites W2122973465 @default.
- W2037013112 cites W2123194290 @default.
- W2037013112 cites W2123401358 @default.
- W2037013112 cites W2124589549 @default.
- W2037013112 cites W2127261774 @default.
- W2037013112 cites W2130754549 @default.
- W2037013112 cites W2131992218 @default.
- W2037013112 cites W2135298445 @default.
- W2037013112 cites W2139813645 @default.
- W2037013112 cites W2141467959 @default.
- W2037013112 cites W2142662767 @default.
- W2037013112 cites W2147337541 @default.
- W2037013112 cites W2152310651 @default.
- W2037013112 cites W2158894328 @default.
- W2037013112 cites W2160004262 @default.
- W2037013112 cites W2161062342 @default.
- W2037013112 cites W2163698469 @default.
- W2037013112 cites W2164862750 @default.
- W2037013112 cites W2170774926 @default.
- W2037013112 doi "https://doi.org/10.1128/jcm.02697-13" @default.
- W2037013112 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3957757" @default.
- W2037013112 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24403298" @default.
- W2037013112 hasPublicationYear "2014" @default.
- W2037013112 type Work @default.
- W2037013112 sameAs 2037013112 @default.
- W2037013112 citedByCount "52" @default.
- W2037013112 countsByYear W20370131122014 @default.
- W2037013112 countsByYear W20370131122015 @default.
- W2037013112 countsByYear W20370131122016 @default.
- W2037013112 countsByYear W20370131122017 @default.
- W2037013112 countsByYear W20370131122018 @default.
- W2037013112 countsByYear W20370131122019 @default.
- W2037013112 countsByYear W20370131122020 @default.
- W2037013112 countsByYear W20370131122021 @default.
- W2037013112 countsByYear W20370131122022 @default.
- W2037013112 countsByYear W20370131122023 @default.
- W2037013112 crossrefType "journal-article" @default.
- W2037013112 hasAuthorship W2037013112A5021808468 @default.
- W2037013112 hasAuthorship W2037013112A5033289218 @default.
- W2037013112 hasAuthorship W2037013112A5034131519 @default.
- W2037013112 hasAuthorship W2037013112A5048497439 @default.
- W2037013112 hasAuthorship W2037013112A5053546594 @default.
- W2037013112 hasAuthorship W2037013112A5063465178 @default.
- W2037013112 hasAuthorship W2037013112A5064345565 @default.
- W2037013112 hasAuthorship W2037013112A5076171817 @default.
- W2037013112 hasAuthorship W2037013112A5084119337 @default.
- W2037013112 hasBestOaLocation W20370131121 @default.
- W2037013112 hasConcept C104317684 @default.
- W2037013112 hasConcept C116675565 @default.
- W2037013112 hasConcept C126322002 @default.
- W2037013112 hasConcept C159047783 @default.
- W2037013112 hasConcept C2776460866 @default.
- W2037013112 hasConcept C2776986154 @default.