FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2037031063> ?p ?o ?g. }

• W2037031063 endingPage "360" @default.
• W2037031063 startingPage "355" @default.
• W2037031063 abstract "Conventional staining of cells or tissue sections on microscope slides involves immersing the slides into solutions of dyes then rinsing to remove the unbound dye. There are instances, however, when use of stain solutions is undesirable-e.g., at microgravity conditions in space, where the possibility of accidental spill (many dyes are known carcinogens) introduces health hazard. Likewise, transporting bulk of liquid stains and rinses may be burdensome in certain situations such as field expeditions or combat.The liquidless staining procedure is proposed in which the dyes are contained in thin strips of hydrated polyacrylamide or gelatin gels that have been presoaked in the stain solutions. Fluorochromes that have affinity to DNA (propidium iodide, PI; 4,6-diamidino-2-phenylindole, DAPI, Hoechst 33342) or to protein (sulforhodamine 101) were used to saturate the gels. The gel strips were placed over the prefixed cells or tissue sections deposited on microscope slides and relatively low (20 g/cm2) pressure was applied to ensure the contact. The cells were also stained by using commercially available mounting media into which DAPI or PI were admixed. Intensity of fluorescence of the PI stained cells was measured by laser scanning cytometry (LSC).Satisfactory cell and tissue staining, with minimal background, was achieved after 10-20 min contact between the cells and gels. Optimal concentrations of the dyes in the solutions used to presoak the gels was found to be 2-4-fold higher than the concentrations used routinely in cytometry. The measurements of intensity of cellular fluorescence by LSC revealed that the staining of DNA was stoichiometric as reflected by the characteristic cellular DNA content frequency histograms with distinct G1, S, and G2/M cell populations and 2:1 ratio of G2/M to G1 peak fluorescence. Individual gels can be saturated with more than a single dye-e.g., to obtain differential DNA and protein staining. Cell staining with DAPI or PI in the gelatin-based mounting media led to high fluorescence background while staining with DAPI in aqueous medium was satisfactory.Relatively fast staining of cells or tissue sections on microscope slides can be achieved by nonconvective dye diffusion using hydrated gels permeated with the dyes, applied to cells at low pressure. The quality of the staining provided by this methodology is comparable to conventional cell staining in dye solutions." @default.
• W2037031063 created "2016-06-24" @default.
• W2037031063 creator A5009873745 @default.
• W2037031063 creator A5059874954 @default.
• W2037031063 creator A5060741746 @default.
• W2037031063 creator A5086304089 @default.
• W2037031063 date "2001-01-01" @default.
• W2037031063 modified "2023-10-10" @default.
• W2037031063 title "?Liquidless? cell staining by dye diffusion from gels and analysis by laser scanning cytometry: Potential application at microgravity conditions in space" @default.
• W2037031063 cites W1537149667 @default.
• W2037031063 cites W1997377729 @default.
• W2037031063 cites W2003855982 @default.
• W2037031063 cites W2008901624 @default.
• W2037031063 cites W2011155657 @default.
• W2037031063 cites W2017536241 @default.
• W2037031063 cites W2020608595 @default.
• W2037031063 cites W2036157162 @default.
• W2037031063 cites W2048872707 @default.
• W2037031063 cites W2049414765 @default.
• W2037031063 cites W2052581310 @default.
• W2037031063 cites W2056098585 @default.
• W2037031063 cites W2085548006 @default.
• W2037031063 cites W2316995248 @default.
• W2037031063 doi "https://doi.org/10.1002/1097-0320(20010801)44:4<355::aid-cyto1127>3.0.co;2-w" @default.
• W2037031063 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11500852" @default.
• W2037031063 hasPublicationYear "2001" @default.
• W2037031063 type Work @default.
• W2037031063 sameAs 2037031063 @default.
• W2037031063 citedByCount "9" @default.
• W2037031063 countsByYear W20370310632017 @default.
• W2037031063 countsByYear W20370310632018 @default.
• W2037031063 countsByYear W20370310632022 @default.
• W2037031063 crossrefType "journal-article" @default.
• W2037031063 hasAuthorship W2037031063A5009873745 @default.
• W2037031063 hasAuthorship W2037031063A5059874954 @default.
• W2037031063 hasAuthorship W2037031063A5060741746 @default.
• W2037031063 hasAuthorship W2037031063A5086304089 @default.
• W2037031063 hasBestOaLocation W20370310631 @default.
• W2037031063 hasConcept C120665830 @default.
• W2037031063 hasConcept C121332964 @default.
• W2037031063 hasConcept C12554922 @default.
• W2037031063 hasConcept C153911025 @default.
• W2037031063 hasConcept C169274487 @default.
• W2037031063 hasConcept C185592680 @default.
• W2037031063 hasConcept C190283241 @default.
• W2037031063 hasConcept C192562407 @default.
• W2037031063 hasConcept C2775934118 @default.
• W2037031063 hasConcept C2777527632 @default.
• W2037031063 hasConcept C2778823387 @default.
• W2037031063 hasConcept C2780339063 @default.
• W2037031063 hasConcept C2781294515 @default.
• W2037031063 hasConcept C31573885 @default.
• W2037031063 hasConcept C43617362 @default.
• W2037031063 hasConcept C54355233 @default.
• W2037031063 hasConcept C553184892 @default.
• W2037031063 hasConcept C55493867 @default.
• W2037031063 hasConcept C74864618 @default.
• W2037031063 hasConcept C86803240 @default.
• W2037031063 hasConcept C91881484 @default.
• W2037031063 hasConceptScore W2037031063C120665830 @default.
• W2037031063 hasConceptScore W2037031063C121332964 @default.
• W2037031063 hasConceptScore W2037031063C12554922 @default.
• W2037031063 hasConceptScore W2037031063C153911025 @default.
• W2037031063 hasConceptScore W2037031063C169274487 @default.
• W2037031063 hasConceptScore W2037031063C185592680 @default.
• W2037031063 hasConceptScore W2037031063C190283241 @default.
• W2037031063 hasConceptScore W2037031063C192562407 @default.
• W2037031063 hasConceptScore W2037031063C2775934118 @default.
• W2037031063 hasConceptScore W2037031063C2777527632 @default.
• W2037031063 hasConceptScore W2037031063C2778823387 @default.
• W2037031063 hasConceptScore W2037031063C2780339063 @default.
• W2037031063 hasConceptScore W2037031063C2781294515 @default.
• W2037031063 hasConceptScore W2037031063C31573885 @default.
• W2037031063 hasConceptScore W2037031063C43617362 @default.
• W2037031063 hasConceptScore W2037031063C54355233 @default.
• W2037031063 hasConceptScore W2037031063C553184892 @default.
• W2037031063 hasConceptScore W2037031063C55493867 @default.
• W2037031063 hasConceptScore W2037031063C74864618 @default.
• W2037031063 hasConceptScore W2037031063C86803240 @default.
• W2037031063 hasConceptScore W2037031063C91881484 @default.
• W2037031063 hasIssue "4" @default.
• W2037031063 hasLocation W20370310631 @default.
• W2037031063 hasLocation W20370310632 @default.
• W2037031063 hasOpenAccess W2037031063 @default.
• W2037031063 hasPrimaryLocation W20370310631 @default.
• W2037031063 hasRelatedWork W1972194178 @default.
• W2037031063 hasRelatedWork W1985238399 @default.
• W2037031063 hasRelatedWork W1991357675 @default.
• W2037031063 hasRelatedWork W2049734679 @default.
• W2037031063 hasRelatedWork W2099335510 @default.
• W2037031063 hasRelatedWork W2321319036 @default.
• W2037031063 hasRelatedWork W2560693721 @default.
• W2037031063 hasRelatedWork W2803301968 @default.
• W2037031063 hasRelatedWork W3082488598 @default.
• W2037031063 hasRelatedWork W4225406382 @default.
• W2037031063 hasVolume "44" @default.
• W2037031063 isParatext "false" @default.
• W2037031063 isRetracted "false" @default.

• next