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• W2037047153 endingPage "606" @default.
• W2037047153 startingPage "601" @default.
• W2037047153 abstract "ABSTRACT We evaluated PCR methods for diagnosis of acute and chronic cutaneous leishmaniasis (CL) in an area of Colombia where Leishmania ( Viannia ) is endemic. The PCR method specifically amplified whole linearized minicircle kinetoplast DNA (kDNA) of the Leishmania subgenus Viannia from biopsy lysates. PCR products were detected in agarose gels. For 255 acute cases, this PCR method had greater sensitivity (75.7%) than each conventional method, i.e., microscopic examination of Giemsa-stained lesion scraping (46.7%), biopsy culture (55.3%), aspirate culture (46.3%), and the conventional methods combined (70.2%). Among 44 cases of chronic CL, amplification of biopsy DNA was more sensitive (45.5%) than the individual (4.5 to 27.7%) and combined (27.3%) conventional methods. The detection of kDNA in biopsies from chronic lesions was enhanced by a chemiluminescent dot blot hybridization, which produced a sensitivity of 65.8% when alone and 90.9% when in combination with DNA extraction of biopsy lysates ( P < 0.001). Three biopsies from 84 skin lesions of other etiologies were falsely positive by PCR (specificity, 96.4%). PCR detected kDNA more frequently in biopsies (detection level, 83.9%) than in aspirates (74.7%) from 103 cases of acute CL. Among aspirates from 53 chronic cases of CL, the alternative methods, DNA extraction and hybridization, increased sensitivity from 41.5 to 56.6% ( P > 0.05). This enhanced PCR method in chronic biopsies was so much more sensitive than conventional methods that it should be considered the preferred diagnostic method for chronic CL. These findings support the appropriate incorporation of PCR into diagnostic strategies for cutaneous leishmaniasis." @default.
• W2037047153 created "2016-06-24" @default.
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• W2037047153 date "2002-02-01" @default.
• W2037047153 modified "2023-09-26" @default.
• W2037047153 title "PCR-Based Diagnosis of Acute and Chronic Cutaneous Leishmaniasis Caused by <i>Leishmania</i> ( <i>Viannia</i> )" @default.
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• W2037047153 doi "https://doi.org/10.1128/jcm.40.2.601-606.2002" @default.
• W2037047153 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/153366" @default.
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