Matches in SemOpenAlex for { <https://semopenalex.org/work/W2037174908> ?p ?o ?g. }
- W2037174908 endingPage "246" @default.
- W2037174908 startingPage "237" @default.
- W2037174908 abstract "We investigated whether neurotoxic effects of the mitochondrial toxin 3-nitropropionic acid (3-NP) in hippocampal slice cultures are dependent on glucose levels in the culture medium and whether such effects occur via apoptosis or necrosis. In addition, 3-NP toxicity was investigated at two developmental stages of the cultures, prepared from rat brain at postnatal day 5-7 and grown in Neurobasal medium for 1 or 3 weeks. Cultures were exposed to 3-NP in the presence of high (25 mM), normal (5 mM), or low (3 mM) glucose for 48 h, followed by 48 h incubation in medium without 3-NP. Cellular propidium iodide (PI) uptake and lactate dehydrogenase (LDH) efflux into the medium revealed time- and dose-dependent cell death by 3-NP, with EC(50) values of about 60 microM in high or normal glucose. Regional vulnerability, as assessed by PI uptake and MAP2 immunostaining, in 3-week-old cultures was as follows: CA1 > CA3 > fascia dentata. In low glucose much lower concentrations of 3-NP (25 microM) triggered neurotoxicity. One-week-old cultures were less susceptible to 3-NP toxicity than 3-week-old cultures, but the dentate granule cells were relatively more affected in the immature cultures. We found no evidence for apoptotic cell death by 3-NP in 3-week-old cultures, but in 1-week-old cultures the putative apoptotic marker c-JUN/AP1 and nuclear fragmentation (Hoechst) were significantly increased in the dentate granule cells." @default.
- W2037174908 created "2016-06-24" @default.
- W2037174908 creator A5018346224 @default.
- W2037174908 creator A5019507056 @default.
- W2037174908 creator A5023573690 @default.
- W2037174908 creator A5073058287 @default.
- W2037174908 creator A5078615730 @default.
- W2037174908 date "2002-07-01" @default.
- W2037174908 modified "2023-10-12" @default.
- W2037174908 title "3-Nitropropionic Acid Neurotoxicity in Hippocampal Slice Cultures: Developmental and Regional Vulnerability and Dependency on Glucose" @default.
- W2037174908 cites W1515735942 @default.
- W2037174908 cites W1554585757 @default.
- W2037174908 cites W1563847798 @default.
- W2037174908 cites W1588823477 @default.
- W2037174908 cites W1822020178 @default.
- W2037174908 cites W1946022517 @default.
- W2037174908 cites W1965792936 @default.
- W2037174908 cites W1967958202 @default.
- W2037174908 cites W1970126151 @default.
- W2037174908 cites W1971585319 @default.
- W2037174908 cites W1972296376 @default.
- W2037174908 cites W1973792860 @default.
- W2037174908 cites W1973987134 @default.
- W2037174908 cites W1976599886 @default.
- W2037174908 cites W1979032829 @default.
- W2037174908 cites W1983133967 @default.
- W2037174908 cites W1983729920 @default.
- W2037174908 cites W1984599311 @default.
- W2037174908 cites W1985370516 @default.
- W2037174908 cites W1994612125 @default.
- W2037174908 cites W1996568613 @default.
- W2037174908 cites W1998470223 @default.
- W2037174908 cites W2001116239 @default.
- W2037174908 cites W2004126223 @default.
- W2037174908 cites W2005423627 @default.
- W2037174908 cites W2005796936 @default.
- W2037174908 cites W2006376420 @default.
- W2037174908 cites W2009213290 @default.
- W2037174908 cites W2010472439 @default.
- W2037174908 cites W2011270545 @default.
- W2037174908 cites W2013083961 @default.
- W2037174908 cites W2021430775 @default.
- W2037174908 cites W2021723653 @default.
- W2037174908 cites W2022316962 @default.
- W2037174908 cites W2023291502 @default.
- W2037174908 cites W2024649641 @default.
- W2037174908 cites W2025959962 @default.
- W2037174908 cites W2033285017 @default.
- W2037174908 cites W2036504478 @default.
- W2037174908 cites W2038511780 @default.
- W2037174908 cites W2039310755 @default.
- W2037174908 cites W2041224810 @default.
- W2037174908 cites W2047482700 @default.
- W2037174908 cites W2047496772 @default.
- W2037174908 cites W2047790179 @default.
- W2037174908 cites W2048724759 @default.
- W2037174908 cites W2051889926 @default.
- W2037174908 cites W2071452513 @default.
- W2037174908 cites W2079054696 @default.
- W2037174908 cites W2080452677 @default.
- W2037174908 cites W2080973723 @default.
- W2037174908 cites W2081791397 @default.
- W2037174908 cites W2086540784 @default.
- W2037174908 cites W2088178681 @default.
- W2037174908 cites W2094242528 @default.
- W2037174908 cites W2095290615 @default.
- W2037174908 cites W2117354575 @default.
- W2037174908 cites W2134265543 @default.
- W2037174908 cites W2145413598 @default.
- W2037174908 cites W2163609268 @default.
- W2037174908 cites W2171617589 @default.
- W2037174908 cites W4211045864 @default.
- W2037174908 cites W50072272 @default.
- W2037174908 cites W964655 @default.
- W2037174908 cites W986461089 @default.
- W2037174908 doi "https://doi.org/10.1006/exnr.2002.7934" @default.
- W2037174908 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12093101" @default.
- W2037174908 hasPublicationYear "2002" @default.
- W2037174908 type Work @default.
- W2037174908 sameAs 2037174908 @default.
- W2037174908 citedByCount "14" @default.
- W2037174908 countsByYear W20371749082015 @default.
- W2037174908 countsByYear W20371749082021 @default.
- W2037174908 countsByYear W20371749082023 @default.
- W2037174908 crossrefType "journal-article" @default.
- W2037174908 hasAuthorship W2037174908A5018346224 @default.
- W2037174908 hasAuthorship W2037174908A5019507056 @default.
- W2037174908 hasAuthorship W2037174908A5023573690 @default.
- W2037174908 hasAuthorship W2037174908A5073058287 @default.
- W2037174908 hasAuthorship W2037174908A5078615730 @default.
- W2037174908 hasConcept C126322002 @default.
- W2037174908 hasConcept C134018914 @default.
- W2037174908 hasConcept C148762608 @default.
- W2037174908 hasConcept C153911025 @default.
- W2037174908 hasConcept C16685009 @default.
- W2037174908 hasConcept C181199279 @default.
- W2037174908 hasConcept C190283241 @default.
- W2037174908 hasConcept C2775934118 @default.