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• W2037193312 abstract "The widely conserved ω subunit encoded by rpoZ is the smallest subunit of Escherichia coli RNA polymerase (RNAP) but is dispensable for bacterial growth. Function of ω is known to be substituted by GroEL in ω-null strain, which thus does not exhibit a discernable phenotype. In this work, we report isolation of ω variants whose expression in vivo leads to a dominant lethal phenotype. Studies show that in contrast to ω, which is largely unstructured, ω mutants display substantial acquisition of secondary structure. By detailed study with one of the mutants, ω6 bearing N60D substitution, the mechanism of lethality has been deciphered. Biochemical analysis reveals that ω6 binds to β′ subunit in vitro with greater affinity than that of ω. The reconstituted RNAP holoenzyme in the presence of ω6 in vitro is defective in transcription initiation. Formation of a faulty RNAP in the presence of mutant ω results in death of the cell. Furthermore, lethality of ω6 is relieved in cells expressing the rpoC2112 allele encoding β′2112, a variant β′ bearing Y457S substitution, immediately adjacent to the β′ catalytic center. Our results suggest that the enhanced ω6-β′ interaction may perturb the plasticity of the RNAP active center, implicating a role for ω and its flexible state.Background: Although universally conserved, ω is the only nonessential subunit of RNA polymerase, and its function is mostly undetermined.Results: Dominant lethal mutants of ω were isolated and analyzed genetically and biochemically.Conclusion: Mutations in ω that alter its structure interfere with RNA polymerase catalytic properties.Significance: Results suggest a critical role for ω during transcription, bypassing the effects of compensatory proteins. The widely conserved ω subunit encoded by rpoZ is the smallest subunit of Escherichia coli RNA polymerase (RNAP) but is dispensable for bacterial growth. Function of ω is known to be substituted by GroEL in ω-null strain, which thus does not exhibit a discernable phenotype. In this work, we report isolation of ω variants whose expression in vivo leads to a dominant lethal phenotype. Studies show that in contrast to ω, which is largely unstructured, ω mutants display substantial acquisition of secondary structure. By detailed study with one of the mutants, ω6 bearing N60D substitution, the mechanism of lethality has been deciphered. Biochemical analysis reveals that ω6 binds to β′ subunit in vitro with greater affinity than that of ω. The reconstituted RNAP holoenzyme in the presence of ω6 in vitro is defective in transcription initiation. Formation of a faulty RNAP in the presence of mutant ω results in death of the cell. Furthermore, lethality of ω6 is relieved in cells expressing the rpoC2112 allele encoding β′2112, a variant β′ bearing Y457S substitution, immediately adjacent to the β′ catalytic center. Our results suggest that the enhanced ω6-β′ interaction may perturb the plasticity of the RNAP active center, implicating a role for ω and its flexible state. Background: Although universally conserved, ω is the only nonessential subunit of RNA polymerase, and its function is mostly undetermined. Results: Dominant lethal mutants of ω were isolated and analyzed genetically and biochemically. Conclusion: Mutations in ω that alter its structure interfere with RNA polymerase catalytic properties. Significance: Results suggest a critical role for ω during transcription, bypassing the effects of compensatory proteins." @default.
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• W2037193312 date "2013-08-01" @default.
• W2037193312 modified "2023-09-30" @default.
• W2037193312 title "Inactivation of the Bacterial RNA Polymerase Due to Acquisition of Secondary Structure by the ω Subunit" @default.
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• W2037193312 doi "https://doi.org/10.1074/jbc.m113.468520" @default.
• W2037193312 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3757172" @default.
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