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- W2037222061 abstract "This work presents a kinetic study on irreversible inhibition of trypsin by TLCK, using a new experimental approach. The method consists of the incubation of the enzyme with an irreversible inhibitor in the presence of a substrate which allows enzyme turnover as well as continuous measurement of the appearance of the product, a simultaneous change in the initial concentrations of the irreversible inhibitor and enzyme being undertaken, though a constant ratio between the latter, is maintained. This new approach enables the kinetic constants for TLCK, k2 and K1, to be determined." @default.
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- W2037222061 date "1986-01-01" @default.
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- W2037222061 title "Irreversible inhibition of trypsin by tlck. A continuous method for kinetic study of irreversible enzymatic inhibitors in the presence of substrate" @default.
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- W2037222061 doi "https://doi.org/10.1016/0020-711x(86)90121-7" @default.
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