Matches in SemOpenAlex for { <https://semopenalex.org/work/W2037226897> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W2037226897 endingPage "13a" @default.
- W2037226897 startingPage "13a" @default.
- W2037226897 abstract "Eukaryotic initiation factor eIF4A is a prototype protein of the DEAD box family of RNA helicases, and is part of the translation initiation complex eIF4F. eIF4A binds to the 5′ cap of mRNA and unwinds structures in the 5′-untranslated regions of mRNAs in ATP dependent manner. Our long-term goal in this project is to decipher the role of the initiation complex eIF4F in ribosomal recruitment, and develop methods to control this process. Although eIF4A has been studied extensively by classical bulk biochemical methods, a direct, unambiguous measurement of its helicase activity and its processivity has not been reported. Here, we use single molecule fluorescence assays to visualize its binding to RNA and melting secondary structures in RNA. Specifically, FRET efficiency dynamics is used to explore the binding location of eIF4A and its unwinding function. We demonstrate that eIF4A does not move on single stranded region, it preferentially binds at a close proximity to the single-strand (ss) / duplex junction on substrates with ssRNA overhangs. We seek to elucidate any elementary steps and kinetic mechanisms involved with eIF4A unwinding of RNA. Single-molecule FRET values decrease with a discrete pattern corresponding to the number of steps for unwinding. We observe the intermediate FRET states in various substrates and conclude that eIF4A unwinds 6 base pairs per step. The processivity of eIF4A increases in the presence of cofactors such as eIF4H. Furthermore, we selectively probe eIF4A activity with small-molecule inhibitor pateamine which stimulates eIF4A activity." @default.
- W2037226897 created "2016-06-24" @default.
- W2037226897 creator A5001399556 @default.
- W2037226897 creator A5003180767 @default.
- W2037226897 creator A5048629507 @default.
- W2037226897 creator A5052762025 @default.
- W2037226897 creator A5074314767 @default.
- W2037226897 date "2010-01-01" @default.
- W2037226897 modified "2023-09-26" @default.
- W2037226897 title "Single Molecule Studies Revealing the Dynamics of RNA Helicase eIF4A" @default.
- W2037226897 doi "https://doi.org/10.1016/j.bpj.2009.12.076" @default.
- W2037226897 hasPublicationYear "2010" @default.
- W2037226897 type Work @default.
- W2037226897 sameAs 2037226897 @default.
- W2037226897 citedByCount "0" @default.
- W2037226897 crossrefType "journal-article" @default.
- W2037226897 hasAuthorship W2037226897A5001399556 @default.
- W2037226897 hasAuthorship W2037226897A5003180767 @default.
- W2037226897 hasAuthorship W2037226897A5048629507 @default.
- W2037226897 hasAuthorship W2037226897A5052762025 @default.
- W2037226897 hasAuthorship W2037226897A5074314767 @default.
- W2037226897 hasBestOaLocation W20372268971 @default.
- W2037226897 hasConcept C104317684 @default.
- W2037226897 hasConcept C105580179 @default.
- W2037226897 hasConcept C121332964 @default.
- W2037226897 hasConcept C12554922 @default.
- W2037226897 hasConcept C131140672 @default.
- W2037226897 hasConcept C149364088 @default.
- W2037226897 hasConcept C161223559 @default.
- W2037226897 hasConcept C2776054693 @default.
- W2037226897 hasConcept C2781455991 @default.
- W2037226897 hasConcept C4718897 @default.
- W2037226897 hasConcept C55493867 @default.
- W2037226897 hasConcept C62520636 @default.
- W2037226897 hasConcept C67705224 @default.
- W2037226897 hasConcept C68592252 @default.
- W2037226897 hasConcept C82381507 @default.
- W2037226897 hasConcept C86803240 @default.
- W2037226897 hasConcept C88478588 @default.
- W2037226897 hasConcept C91881484 @default.
- W2037226897 hasConcept C95444343 @default.
- W2037226897 hasConcept C96305047 @default.
- W2037226897 hasConcept C99348085 @default.
- W2037226897 hasConceptScore W2037226897C104317684 @default.
- W2037226897 hasConceptScore W2037226897C105580179 @default.
- W2037226897 hasConceptScore W2037226897C121332964 @default.
- W2037226897 hasConceptScore W2037226897C12554922 @default.
- W2037226897 hasConceptScore W2037226897C131140672 @default.
- W2037226897 hasConceptScore W2037226897C149364088 @default.
- W2037226897 hasConceptScore W2037226897C161223559 @default.
- W2037226897 hasConceptScore W2037226897C2776054693 @default.
- W2037226897 hasConceptScore W2037226897C2781455991 @default.
- W2037226897 hasConceptScore W2037226897C4718897 @default.
- W2037226897 hasConceptScore W2037226897C55493867 @default.
- W2037226897 hasConceptScore W2037226897C62520636 @default.
- W2037226897 hasConceptScore W2037226897C67705224 @default.
- W2037226897 hasConceptScore W2037226897C68592252 @default.
- W2037226897 hasConceptScore W2037226897C82381507 @default.
- W2037226897 hasConceptScore W2037226897C86803240 @default.
- W2037226897 hasConceptScore W2037226897C88478588 @default.
- W2037226897 hasConceptScore W2037226897C91881484 @default.
- W2037226897 hasConceptScore W2037226897C95444343 @default.
- W2037226897 hasConceptScore W2037226897C96305047 @default.
- W2037226897 hasConceptScore W2037226897C99348085 @default.
- W2037226897 hasIssue "3" @default.
- W2037226897 hasLocation W20372268971 @default.
- W2037226897 hasOpenAccess W2037226897 @default.
- W2037226897 hasPrimaryLocation W20372268971 @default.
- W2037226897 hasRelatedWork W1454765678 @default.
- W2037226897 hasRelatedWork W1545434940 @default.
- W2037226897 hasRelatedWork W2002961201 @default.
- W2037226897 hasRelatedWork W2037226897 @default.
- W2037226897 hasRelatedWork W2159353859 @default.
- W2037226897 hasRelatedWork W2324112111 @default.
- W2037226897 hasRelatedWork W2585015165 @default.
- W2037226897 hasRelatedWork W2617187727 @default.
- W2037226897 hasRelatedWork W2886180158 @default.
- W2037226897 hasRelatedWork W4282915478 @default.
- W2037226897 hasVolume "98" @default.
- W2037226897 isParatext "false" @default.
- W2037226897 isRetracted "false" @default.
- W2037226897 magId "2037226897" @default.
- W2037226897 workType "article" @default.