FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2037249936> ?p ?o ?g. }

• W2037249936 endingPage "2068" @default.
• W2037249936 startingPage "2060" @default.
• W2037249936 abstract "We have previously reported that maximal platelet activation with adenosine diphosphate (100 microM ADP) causes rapid expression of all GPIIb-IIIa receptors for fibrinogen (FgR) (< 1-3 s), measured with FITC-labeled PAC1 by flow cytometry. We have extended these studies to examine the effects of ADP concentration on the graded expression and Fg occupancy of GPIIb-IIIa receptors. Human citrated platelet-rich plasma, diluted 10-fold with Walsh-albumin-Mg+2 (2 mM), was treated with ADP (0.1-100 microM). The rates of GPIIb-IIIa receptor expression or Fg binding were measured in unstirred samples by flow cytometry, using FITC-labeled monoclonal antibodies (mAb) PAC1 and 9F9, respectively, from on-rates, using increasing times between mAb and ADP additions. Fibrinogen receptors were all expressed rapidly at low (1 microM) or high (100 microM) ADP (few seconds), whereas Fg occupancy was 50% of maximal by about 2 min. The maximal extent of GPIIb-IIIa receptor expression and Fg occupancy was determined from maximal binding (Flmax) at 30 min incubation with PAC1 or 9F9. On-rates and maximal extents of binding for either PAC1 or 9F9 probes showed identical [ADP]-response profiles (KD approximately 1.4 +/- 0.1 microM). However, Flmax studies showed bimodal histograms consisting of resting (Po) and maximally activated (P*) platelets for both PAC1 and 9F9 binding, with the fraction of activated platelets increasing with ADP concentration. The data best fit a model where platelet subpopulations are quantally transformed from Po to P*, expressing all GPIIb-IIIa receptors, rapidly filled by Fg, but triggered at critical ADP concentrations. Larger, but not the largest, platelets appear to be the most sensitive subpopulation. The implications for clinical studies are discussed, and the relationship to dynamics of aggregation are described in a companion paper." @default.
• W2037249936 created "2016-06-24" @default.
• W2037249936 creator A5040626596 @default.
• W2037249936 creator A5075964419 @default.
• W2037249936 creator A5088685783 @default.
• W2037249936 date "1994-11-01" @default.
• W2037249936 modified "2023-10-18" @default.
• W2037249936 title "Dynamics of platelet glycoprotein IIb-IIIa receptor expression and fibrinogen binding. I. Quantal activation of platelet subpopulations varies with adenosine diphosphate concentration" @default.
• W2037249936 cites W107925600 @default.
• W2037249936 cites W122341406 @default.
• W2037249936 cites W1234014522 @default.
• W2037249936 cites W133668382 @default.
• W2037249936 cites W1482855479 @default.
• W2037249936 cites W1584814201 @default.
• W2037249936 cites W1601529928 @default.
• W2037249936 cites W1789531821 @default.
• W2037249936 cites W1971656806 @default.
• W2037249936 cites W1972359811 @default.
• W2037249936 cites W1978051018 @default.
• W2037249936 cites W2003661065 @default.
• W2037249936 cites W2031143871 @default.
• W2037249936 cites W2044268074 @default.
• W2037249936 cites W2076179882 @default.
• W2037249936 cites W2076667548 @default.
• W2037249936 cites W207951093 @default.
• W2037249936 cites W2093120318 @default.
• W2037249936 cites W2093420819 @default.
• W2037249936 cites W2108277570 @default.
• W2037249936 cites W2123979929 @default.
• W2037249936 cites W2327484480 @default.
• W2037249936 cites W2407299336 @default.
• W2037249936 cites W2421932957 @default.
• W2037249936 cites W25667380 @default.
• W2037249936 cites W2887455519 @default.
• W2037249936 cites W53782171 @default.
• W2037249936 doi "https://doi.org/10.1016/s0006-3495(94)80689-7" @default.
• W2037249936 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1225581" @default.
• W2037249936 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7858143" @default.
• W2037249936 hasPublicationYear "1994" @default.
• W2037249936 type Work @default.
• W2037249936 sameAs 2037249936 @default.
• W2037249936 citedByCount "49" @default.
• W2037249936 countsByYear W20372499362012 @default.
• W2037249936 countsByYear W20372499362013 @default.
• W2037249936 countsByYear W20372499362014 @default.
• W2037249936 countsByYear W20372499362015 @default.
• W2037249936 countsByYear W20372499362016 @default.
• W2037249936 countsByYear W20372499362017 @default.
• W2037249936 countsByYear W20372499362018 @default.
• W2037249936 countsByYear W20372499362019 @default.
• W2037249936 countsByYear W20372499362020 @default.
• W2037249936 countsByYear W20372499362021 @default.
• W2037249936 countsByYear W20372499362022 @default.
• W2037249936 crossrefType "journal-article" @default.
• W2037249936 hasAuthorship W2037249936A5040626596 @default.
• W2037249936 hasAuthorship W2037249936A5075964419 @default.
• W2037249936 hasAuthorship W2037249936A5088685783 @default.
• W2037249936 hasBestOaLocation W20372499361 @default.
• W2037249936 hasConcept C12554922 @default.
• W2037249936 hasConcept C126322002 @default.
• W2037249936 hasConcept C153911025 @default.
• W2037249936 hasConcept C170493617 @default.
• W2037249936 hasConcept C185592680 @default.
• W2037249936 hasConcept C26828662 @default.
• W2037249936 hasConcept C2777399203 @default.
• W2037249936 hasConcept C2779036427 @default.
• W2037249936 hasConcept C2993802102 @default.
• W2037249936 hasConcept C3018697912 @default.
• W2037249936 hasConcept C553184892 @default.
• W2037249936 hasConcept C55493867 @default.
• W2037249936 hasConcept C71924100 @default.
• W2037249936 hasConcept C86803240 @default.
• W2037249936 hasConcept C89560881 @default.
• W2037249936 hasConceptScore W2037249936C12554922 @default.
• W2037249936 hasConceptScore W2037249936C126322002 @default.
• W2037249936 hasConceptScore W2037249936C153911025 @default.
• W2037249936 hasConceptScore W2037249936C170493617 @default.
• W2037249936 hasConceptScore W2037249936C185592680 @default.
• W2037249936 hasConceptScore W2037249936C26828662 @default.
• W2037249936 hasConceptScore W2037249936C2777399203 @default.
• W2037249936 hasConceptScore W2037249936C2779036427 @default.
• W2037249936 hasConceptScore W2037249936C2993802102 @default.
• W2037249936 hasConceptScore W2037249936C3018697912 @default.
• W2037249936 hasConceptScore W2037249936C553184892 @default.
• W2037249936 hasConceptScore W2037249936C55493867 @default.
• W2037249936 hasConceptScore W2037249936C71924100 @default.
• W2037249936 hasConceptScore W2037249936C86803240 @default.
• W2037249936 hasConceptScore W2037249936C89560881 @default.
• W2037249936 hasIssue "5" @default.
• W2037249936 hasLocation W20372499361 @default.
• W2037249936 hasLocation W20372499362 @default.
• W2037249936 hasLocation W20372499363 @default.
• W2037249936 hasLocation W20372499364 @default.
• W2037249936 hasOpenAccess W2037249936 @default.
• W2037249936 hasPrimaryLocation W20372499361 @default.
• W2037249936 hasRelatedWork W1986282677 @default.
• W2037249936 hasRelatedWork W1993046381 @default.
• W2037249936 hasRelatedWork W1998759773 @default.

• next