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- W2037251036 abstract "Proteasomes (prosomes) of calf-liver cells were probed with three different biotinylated lectins: Limulus polyphemus agglutinin (LPA), specific for neuraminic acid; Solanum tuberosum agglutinin (STA), specific for GlcNac; and concanavalin A (Con A), specific for Man/Glc. While only one proteasomal protein reacted with STA, most of the proteasomal proteins reacted with LPA and several with Con A. Deglycosylation with N-glycosidase F showed that the detected glycan residues were asparagine-linked. Finally we demonstrate an alternative method for the isolation of proteasomes based on the affinity of certain proteasomal proteins to Con A." @default.
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- W2037251036 date "1993-01-01" @default.
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- W2037251036 title "Glycosylation and deglycosylation of proteasomes (prosomes) from calf-liver cells: High abundance of neuraminic acid" @default.
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- W2037251036 doi "https://doi.org/10.1016/0300-9084(93)90047-v" @default.
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