FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2037260827> ?p ?o ?g. }

• W2037260827 endingPage "269" @default.
• W2037260827 startingPage "257" @default.
• W2037260827 abstract "The human Y4 receptor, a class A G-protein coupled receptor (GPCR) primarily targeted by the pancreatic polypeptide (PP), is involved in a large number of physiologically important functions. This paper investigates a Y4 receptor fragment (N-TM1-TM2) comprising the N-terminal domain, the first two transmembrane (TM) helices and the first extracellular loop followed by a (His)(6) tag, and addresses synthetic problems encountered when recombinantly producing such fragments from GPCRs in Escherichia coli. Rigorous purification and usage of the optimized detergent mixture 28 mM dodecylphosphocholine (DPC)/118 mM% 1-palmitoyl-2-hydroxy-sn-glycero-3-[phospho-rac-(1-glycerol)] (LPPG) resulted in high quality TROSY spectra indicating protein conformational homogeneity. Almost complete assignment of the backbone, including all TM residue resonances was obtained. Data on internal backbone dynamics revealed a high secondary structure content for N-TM1-TM2. Secondary chemical shifts and sequential amide proton nuclear Overhauser effects defined the TM helices. Interestingly, the properties of the N-terminal domain of this large fragment are highly similar to those determined on the isolated N-terminal domain in the presence of DPC micelles." @default.
• W2037260827 created "2016-06-24" @default.
• W2037260827 creator A5008534508 @default.
• W2037260827 creator A5044184936 @default.
• W2037260827 creator A5049373174 @default.
• W2037260827 date "2008-10-21" @default.
• W2037260827 modified "2023-10-16" @default.
• W2037260827 title "Biosynthesis and NMR-studies of a double transmembrane domain from the Y4 receptor, a human GPCR" @default.
• W2037260827 cites W1489895184 @default.
• W2037260827 cites W1537290919 @default.
• W2037260827 cites W1589295940 @default.
• W2037260827 cites W1589804742 @default.
• W2037260827 cites W1908561802 @default.
• W2037260827 cites W1969949441 @default.
• W2037260827 cites W1971886200 @default.
• W2037260827 cites W1972923838 @default.
• W2037260827 cites W1973969870 @default.
• W2037260827 cites W1990675281 @default.
• W2037260827 cites W1990889430 @default.
• W2037260827 cites W1992144784 @default.
• W2037260827 cites W1995352918 @default.
• W2037260827 cites W1996478334 @default.
• W2037260827 cites W1997729584 @default.
• W2037260827 cites W2000565142 @default.
• W2037260827 cites W2005913521 @default.
• W2037260827 cites W2006634380 @default.
• W2037260827 cites W2007089493 @default.
• W2037260827 cites W2011319378 @default.
• W2037260827 cites W2015533931 @default.
• W2037260827 cites W2018327722 @default.
• W2037260827 cites W2022262813 @default.
• W2037260827 cites W2022496873 @default.
• W2037260827 cites W2023291722 @default.
• W2037260827 cites W2023492046 @default.
• W2037260827 cites W2025481401 @default.
• W2037260827 cites W2025557146 @default.
• W2037260827 cites W2025859600 @default.
• W2037260827 cites W2027704563 @default.
• W2037260827 cites W2027711565 @default.
• W2037260827 cites W2030813859 @default.
• W2037260827 cites W2031981850 @default.
• W2037260827 cites W2032569777 @default.
• W2037260827 cites W2032790231 @default.
• W2037260827 cites W2036970696 @default.
• W2037260827 cites W2037960060 @default.
• W2037260827 cites W2038155906 @default.
• W2037260827 cites W2038346739 @default.
• W2037260827 cites W2041566506 @default.
• W2037260827 cites W2043466024 @default.
• W2037260827 cites W2045950270 @default.
• W2037260827 cites W2047345842 @default.
• W2037260827 cites W2048837038 @default.
• W2037260827 cites W2052711005 @default.
• W2037260827 cites W2057437570 @default.
• W2037260827 cites W2065335146 @default.
• W2037260827 cites W2066058955 @default.
• W2037260827 cites W2067889691 @default.
• W2037260827 cites W2068227670 @default.
• W2037260827 cites W2069696932 @default.
• W2037260827 cites W2074370114 @default.
• W2037260827 cites W2074545668 @default.
• W2037260827 cites W2075686927 @default.
• W2037260827 cites W2079256912 @default.
• W2037260827 cites W2080231167 @default.
• W2037260827 cites W2081030911 @default.
• W2037260827 cites W2082486233 @default.
• W2037260827 cites W2084306775 @default.
• W2037260827 cites W2084325913 @default.
• W2037260827 cites W2092436603 @default.
• W2037260827 cites W2096331217 @default.
• W2037260827 cites W2099927887 @default.
• W2037260827 cites W2100036574 @default.
• W2037260827 cites W2100147128 @default.
• W2037260827 cites W2101871915 @default.
• W2037260827 cites W2104477830 @default.
• W2037260827 cites W2105115207 @default.
• W2037260827 cites W2108707223 @default.
• W2037260827 cites W2108743747 @default.
• W2037260827 cites W2121381591 @default.
• W2037260827 cites W2127230663 @default.
• W2037260827 cites W2127871849 @default.
• W2037260827 cites W2140583034 @default.
• W2037260827 cites W2140770667 @default.
• W2037260827 cites W2147281292 @default.
• W2037260827 cites W2148521301 @default.
• W2037260827 cites W2149963584 @default.
• W2037260827 cites W2155147384 @default.
• W2037260827 cites W2155457373 @default.
• W2037260827 cites W2160535028 @default.
• W2037260827 cites W2164192718 @default.
• W2037260827 cites W2171501765 @default.
• W2037260827 cites W22264255 @default.
• W2037260827 cites W4241887033 @default.
• W2037260827 cites W72561416 @default.
• W2037260827 doi "https://doi.org/10.1007/s10858-008-9281-z" @default.
• W2037260827 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18937032" @default.
• W2037260827 hasPublicationYear "2008" @default.
• W2037260827 type Work @default.

• next