FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2037289488> ?p ?o ?g. }

• W2037289488 endingPage "76" @default.
• W2037289488 startingPage "67" @default.
• W2037289488 abstract "Actin filaments and microtubules, both in situ and in vitro, were imaged using high-resolution scanning electron microscopy (HRSEM) at low temperature. For visualization of cytoskeletal elements in situ, fibroblasts were first extracted and fixed; for cytoskeletal elements in vitro, purified proteins were polymerized and fixed. Both types of specimen were then subjected to plunge freezing, controlled freeze-drying, cryo-sputter coating with a thin chromium layer, cryo-transferring and cryo-observation in an FESEM. The three-dimensional architecture of the cytoskeleton was well preserved, permitting examination of the structural relationships among cytoskeletal elements. Actin filaments and microtubules were identified by their characteristic helical features. Two periodicities of actin filaments, the short pitch of the left-handed helix measured at 5.5 nm and the 37-nm-long pitch helix, were revealed. Individual protofilaments were seen in microtubules as well as the characteristic 4-nm repeat of tubulin subunits along the protofilament. Clathrin cages were also observed. This technique provides a powerful approach for direct imaging of macromolecular structures with high contrast and high signal-to-noise ratio at a resolution of 2-3 nm." @default.
• W2037289488 created "2016-06-24" @default.
• W2037289488 creator A5002316771 @default.
• W2037289488 creator A5056378254 @default.
• W2037289488 creator A5086297312 @default.
• W2037289488 creator A5090386437 @default.
• W2037289488 date "1995-07-01" @default.
• W2037289488 modified "2023-10-13" @default.
• W2037289488 title "Imaging of cytoskeletal elements by low-temperature high-resolution scanning electron microscopy" @default.
• W2037289488 cites W121504470 @default.
• W2037289488 cites W127842574 @default.
• W2037289488 cites W128480413 @default.
• W2037289488 cites W1465301386 @default.
• W2037289488 cites W1538692246 @default.
• W2037289488 cites W1976580648 @default.
• W2037289488 cites W1977209986 @default.
• W2037289488 cites W1983169741 @default.
• W2037289488 cites W1988851133 @default.
• W2037289488 cites W2000122627 @default.
• W2037289488 cites W2000438653 @default.
• W2037289488 cites W2001837643 @default.
• W2037289488 cites W2007961864 @default.
• W2037289488 cites W2010067036 @default.
• W2037289488 cites W2013409383 @default.
• W2037289488 cites W2019969744 @default.
• W2037289488 cites W2027321010 @default.
• W2037289488 cites W2031344439 @default.
• W2037289488 cites W2031687258 @default.
• W2037289488 cites W2038920575 @default.
• W2037289488 cites W2042175080 @default.
• W2037289488 cites W2042206217 @default.
• W2037289488 cites W204525914 @default.
• W2037289488 cites W2046563064 @default.
• W2037289488 cites W2048663799 @default.
• W2037289488 cites W2057949435 @default.
• W2037289488 cites W2063178736 @default.
• W2037289488 cites W2069895646 @default.
• W2037289488 cites W2070538072 @default.
• W2037289488 cites W2073708642 @default.
• W2037289488 cites W2075770945 @default.
• W2037289488 cites W2078638471 @default.
• W2037289488 cites W2084475801 @default.
• W2037289488 cites W2084522254 @default.
• W2037289488 cites W2088674363 @default.
• W2037289488 cites W2090772323 @default.
• W2037289488 cites W2105773551 @default.
• W2037289488 cites W2113824777 @default.
• W2037289488 cites W2123479098 @default.
• W2037289488 cites W2131864418 @default.
• W2037289488 cites W2132637089 @default.
• W2037289488 cites W2143752323 @default.
• W2037289488 cites W2219169508 @default.
• W2037289488 cites W4205809008 @default.
• W2037289488 cites W644669495 @default.
• W2037289488 cites W93877194 @default.
• W2037289488 doi "https://doi.org/10.1111/j.1365-2818.1995.tb03613.x" @default.
• W2037289488 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7666412" @default.
• W2037289488 hasPublicationYear "1995" @default.
• W2037289488 type Work @default.
• W2037289488 sameAs 2037289488 @default.
• W2037289488 citedByCount "25" @default.
• W2037289488 countsByYear W20372894882012 @default.
• W2037289488 countsByYear W20372894882013 @default.
• W2037289488 countsByYear W20372894882020 @default.
• W2037289488 crossrefType "journal-article" @default.
• W2037289488 hasAuthorship W2037289488A5002316771 @default.
• W2037289488 hasAuthorship W2037289488A5056378254 @default.
• W2037289488 hasAuthorship W2037289488A5086297312 @default.
• W2037289488 hasAuthorship W2037289488A5090386437 @default.
• W2037289488 hasConcept C120665830 @default.
• W2037289488 hasConcept C121332964 @default.
• W2037289488 hasConcept C12554922 @default.
• W2037289488 hasConcept C125705527 @default.
• W2037289488 hasConcept C138268822 @default.
• W2037289488 hasConcept C142669718 @default.
• W2037289488 hasConcept C147080431 @default.
• W2037289488 hasConcept C1491633281 @default.
• W2037289488 hasConcept C154945302 @default.
• W2037289488 hasConcept C157207234 @default.
• W2037289488 hasConcept C159985019 @default.
• W2037289488 hasConcept C185592680 @default.
• W2037289488 hasConcept C192562407 @default.
• W2037289488 hasConcept C203014093 @default.
• W2037289488 hasConcept C20418707 @default.
• W2037289488 hasConcept C204232928 @default.
• W2037289488 hasConcept C26771246 @default.
• W2037289488 hasConcept C41008148 @default.
• W2037289488 hasConcept C55493867 @default.
• W2037289488 hasConcept C84425145 @default.
• W2037289488 hasConcept C86803240 @default.
• W2037289488 hasConcept C93877712 @default.
• W2037289488 hasConcept C95444343 @default.
• W2037289488 hasConceptScore W2037289488C120665830 @default.
• W2037289488 hasConceptScore W2037289488C121332964 @default.
• W2037289488 hasConceptScore W2037289488C12554922 @default.
• W2037289488 hasConceptScore W2037289488C125705527 @default.
• W2037289488 hasConceptScore W2037289488C138268822 @default.
• W2037289488 hasConceptScore W2037289488C142669718 @default.

• next