Matches in SemOpenAlex for { <https://semopenalex.org/work/W2037292215> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2037292215 endingPage "2243" @default.
- W2037292215 startingPage "2239" @default.
- W2037292215 abstract "Recently, oxidation products of linoleic acid such as 13-hydroxyoctadecadienoic acid (HODE) have been implicated in the regulation of cellular physiology including the proliferative response to growth factor treatment. In addition, an NAD+-dependent 13-HODE dehydrogenase was recently described. To evaluate the contribution of this enzyme to cellular processes we have examined the behavior of the enzyme under different conditions. In the present report, changes in the activity of 13-hydroxyoctadecadienoic acid dehydrogenase during in vitro differentiation of two different cell lines were examined. The cell line HT-29 undergoes induced differentiation via manipulation of the medium while the Caco-2 line undergoes spontaneous differentiation upon attainment of confluence. In both cell lines, longer culture times were accompanied by increases in 13-HODE dehydrogenase activity. The increase in enzyme activity continued even after cell proliferation had ceased. Cellular differentiation was verified by the observation of increases in sucrase and alkaline phosphatase activities. In addition, the activity of 13-HODE dehydrogenase was measured in growing, early confluent and late confluent cultures of undifferentiating Swiss mouse 3T3 fibroblasts. In the fibroblast line, no significant changes in 13-HODE dehydrogenase activity were observed during the course of the experiment. The specific activity of 13-HODE dehydrogenase was also significantly different between the three cell lines, consistent with the extent of differentiation. Highest levels of activity were found in Caco-2 cells (200–400 pmol/min/mg), intermediate levels in the HT-29 cells (20–60 pmol/min/mg) and barely detectable levels in the fibroblasts (0.6–2 pmol/min/mg). The correlation between 13-HODE dehydrogenase and cell differentiation suggests the enzyme may have a role to play in the partitioning of cells between proliferation and differentiation pathways." @default.
- W2037292215 created "2016-06-24" @default.
- W2037292215 creator A5006562145 @default.
- W2037292215 creator A5009586491 @default.
- W2037292215 creator A5046040162 @default.
- W2037292215 creator A5068251176 @default.
- W2037292215 creator A5088432120 @default.
- W2037292215 date "1993-01-01" @default.
- W2037292215 modified "2023-10-03" @default.
- W2037292215 title "Increases in 13-hydroxyoctadecadienoic acid dehydrogenase activity during differentiation of cultured cells" @default.
- W2037292215 doi "https://doi.org/10.1093/carcin/14.11.2239" @default.
- W2037292215 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8242849" @default.
- W2037292215 hasPublicationYear "1993" @default.
- W2037292215 type Work @default.
- W2037292215 sameAs 2037292215 @default.
- W2037292215 citedByCount "24" @default.
- W2037292215 countsByYear W20372922152014 @default.
- W2037292215 countsByYear W20372922152018 @default.
- W2037292215 countsByYear W20372922152019 @default.
- W2037292215 crossrefType "journal-article" @default.
- W2037292215 hasAuthorship W2037292215A5006562145 @default.
- W2037292215 hasAuthorship W2037292215A5009586491 @default.
- W2037292215 hasAuthorship W2037292215A5046040162 @default.
- W2037292215 hasAuthorship W2037292215A5068251176 @default.
- W2037292215 hasAuthorship W2037292215A5088432120 @default.
- W2037292215 hasConcept C104317684 @default.
- W2037292215 hasConcept C148738053 @default.
- W2037292215 hasConcept C153911025 @default.
- W2037292215 hasConcept C160160445 @default.
- W2037292215 hasConcept C181199279 @default.
- W2037292215 hasConcept C185592680 @default.
- W2037292215 hasConcept C202751555 @default.
- W2037292215 hasConcept C2776317432 @default.
- W2037292215 hasConcept C2780381497 @default.
- W2037292215 hasConcept C28212737 @default.
- W2037292215 hasConcept C54355233 @default.
- W2037292215 hasConcept C55493867 @default.
- W2037292215 hasConcept C62112901 @default.
- W2037292215 hasConcept C81885089 @default.
- W2037292215 hasConcept C86803240 @default.
- W2037292215 hasConceptScore W2037292215C104317684 @default.
- W2037292215 hasConceptScore W2037292215C148738053 @default.
- W2037292215 hasConceptScore W2037292215C153911025 @default.
- W2037292215 hasConceptScore W2037292215C160160445 @default.
- W2037292215 hasConceptScore W2037292215C181199279 @default.
- W2037292215 hasConceptScore W2037292215C185592680 @default.
- W2037292215 hasConceptScore W2037292215C202751555 @default.
- W2037292215 hasConceptScore W2037292215C2776317432 @default.
- W2037292215 hasConceptScore W2037292215C2780381497 @default.
- W2037292215 hasConceptScore W2037292215C28212737 @default.
- W2037292215 hasConceptScore W2037292215C54355233 @default.
- W2037292215 hasConceptScore W2037292215C55493867 @default.
- W2037292215 hasConceptScore W2037292215C62112901 @default.
- W2037292215 hasConceptScore W2037292215C81885089 @default.
- W2037292215 hasConceptScore W2037292215C86803240 @default.
- W2037292215 hasIssue "11" @default.
- W2037292215 hasLocation W20372922151 @default.
- W2037292215 hasLocation W20372922152 @default.
- W2037292215 hasOpenAccess W2037292215 @default.
- W2037292215 hasPrimaryLocation W20372922151 @default.
- W2037292215 hasRelatedWork W1656364975 @default.
- W2037292215 hasRelatedWork W1972665833 @default.
- W2037292215 hasRelatedWork W1988346978 @default.
- W2037292215 hasRelatedWork W1999957271 @default.
- W2037292215 hasRelatedWork W2002140570 @default.
- W2037292215 hasRelatedWork W2037292215 @default.
- W2037292215 hasRelatedWork W2078646031 @default.
- W2037292215 hasRelatedWork W2114819079 @default.
- W2037292215 hasRelatedWork W2204845950 @default.
- W2037292215 hasRelatedWork W2380989154 @default.
- W2037292215 hasVolume "14" @default.
- W2037292215 isParatext "false" @default.
- W2037292215 isRetracted "false" @default.
- W2037292215 magId "2037292215" @default.
- W2037292215 workType "article" @default.