Matches in SemOpenAlex for { <https://semopenalex.org/work/W2037299871> ?p ?o ?g. }
- W2037299871 endingPage "216" @default.
- W2037299871 startingPage "198" @default.
- W2037299871 abstract "For RNA to fold into compact, ordered structures, it must overcome electrostatic repulsion between negatively charged phosphate groups by counterion recruitment. A physical understanding of the counterion-assisted folding process requires addressing how cations kinetically and thermodynamically control the folding equilibrium for each tertiary interaction in a full‐length RNA. In this work, single-molecule FRET (fluorescence resonance energy transfer) techniques are exploited to isolate and explore the cation-concentration‐dependent kinetics for formation of a ubiquitous RNA tertiary interaction, that is, the docking/undocking of a GAAA tetraloop with its 11‐nt receptor. Rate constants for docking (kdock) and undocking (kundock) are obtained as a function of cation concentration, size, and valence, specifically for the series Na+, K+, Mg2 +, Ca2 +, Co(NH3)63 +, and spermidine3 +. Increasing cation concentration accelerates kdock dramatically but achieves only a slight decrease in kundock. These results can be kinetically modeled using parallel cation-dependent and cation‐independent docking pathways, which allows for isolation of the folding kinetics from the interaction energetics of the cations with the undocked and docked states, respectively. This analysis reveals a preferential interaction of the cations with the transition state and docked state as compared to the undocked RNA, with the ion–RNA interaction strength growing with cation valence. However, the corresponding number of cations that are taken up by the RNA upon folding decreases with charge density of the cation. The only exception to these behaviors is spermidine3 +, whose weaker influence on the docking equilibria with respect to Co(NH3)63 + can be ascribed to steric effects preventing complete neutralization of the RNA phosphate groups." @default.
- W2037299871 created "2016-06-24" @default.
- W2037299871 creator A5002663671 @default.
- W2037299871 creator A5053616792 @default.
- W2037299871 creator A5056538359 @default.
- W2037299871 creator A5077457385 @default.
- W2037299871 creator A5084872177 @default.
- W2037299871 date "2012-10-01" @default.
- W2037299871 modified "2023-09-30" @default.
- W2037299871 title "The Role of Counterion Valence and Size in GAAA Tetraloop–Receptor Docking/Undocking Kinetics" @default.
- W2037299871 cites W1489623410 @default.
- W2037299871 cites W1494641947 @default.
- W2037299871 cites W1498399113 @default.
- W2037299871 cites W1529084957 @default.
- W2037299871 cites W1569973595 @default.
- W2037299871 cites W1575620047 @default.
- W2037299871 cites W1963830843 @default.
- W2037299871 cites W1963907228 @default.
- W2037299871 cites W1966307609 @default.
- W2037299871 cites W1970441908 @default.
- W2037299871 cites W1971217997 @default.
- W2037299871 cites W1972570207 @default.
- W2037299871 cites W1972570758 @default.
- W2037299871 cites W1972704395 @default.
- W2037299871 cites W1976137961 @default.
- W2037299871 cites W1976260423 @default.
- W2037299871 cites W1976281789 @default.
- W2037299871 cites W1981365891 @default.
- W2037299871 cites W1982913950 @default.
- W2037299871 cites W1984357954 @default.
- W2037299871 cites W1988744922 @default.
- W2037299871 cites W1988884185 @default.
- W2037299871 cites W1990447102 @default.
- W2037299871 cites W1992072903 @default.
- W2037299871 cites W1994994309 @default.
- W2037299871 cites W1995499111 @default.
- W2037299871 cites W1996653560 @default.
- W2037299871 cites W1999935755 @default.
- W2037299871 cites W2001226185 @default.
- W2037299871 cites W2006858059 @default.
- W2037299871 cites W2007222863 @default.
- W2037299871 cites W2007286218 @default.
- W2037299871 cites W2013578231 @default.
- W2037299871 cites W2013670308 @default.
- W2037299871 cites W2013902881 @default.
- W2037299871 cites W2014617853 @default.
- W2037299871 cites W2014790936 @default.
- W2037299871 cites W2021462684 @default.
- W2037299871 cites W2023219682 @default.
- W2037299871 cites W2026817175 @default.
- W2037299871 cites W2026884660 @default.
- W2037299871 cites W2026945988 @default.
- W2037299871 cites W2031792542 @default.
- W2037299871 cites W2032642369 @default.
- W2037299871 cites W2033788106 @default.
- W2037299871 cites W2035361771 @default.
- W2037299871 cites W2035448278 @default.
- W2037299871 cites W2039133207 @default.
- W2037299871 cites W2042199161 @default.
- W2037299871 cites W2044247083 @default.
- W2037299871 cites W2047539499 @default.
- W2037299871 cites W2048962220 @default.
- W2037299871 cites W2051468886 @default.
- W2037299871 cites W2053847144 @default.
- W2037299871 cites W2058276221 @default.
- W2037299871 cites W2058627247 @default.
- W2037299871 cites W2061072646 @default.
- W2037299871 cites W2066511094 @default.
- W2037299871 cites W2068662199 @default.
- W2037299871 cites W2070820417 @default.
- W2037299871 cites W2071599863 @default.
- W2037299871 cites W2073406862 @default.
- W2037299871 cites W2074489975 @default.
- W2037299871 cites W2078662619 @default.
- W2037299871 cites W2079532263 @default.
- W2037299871 cites W2079991169 @default.
- W2037299871 cites W2080251448 @default.
- W2037299871 cites W2081125994 @default.
- W2037299871 cites W2081561450 @default.
- W2037299871 cites W2083364776 @default.
- W2037299871 cites W2085794569 @default.
- W2037299871 cites W2085903676 @default.
- W2037299871 cites W2092144432 @default.
- W2037299871 cites W2094517427 @default.
- W2037299871 cites W2098287259 @default.
- W2037299871 cites W2101996964 @default.
- W2037299871 cites W2102032812 @default.
- W2037299871 cites W2102834112 @default.
- W2037299871 cites W2110408122 @default.
- W2037299871 cites W2110790388 @default.
- W2037299871 cites W2111652614 @default.
- W2037299871 cites W2114562270 @default.
- W2037299871 cites W2118956677 @default.
- W2037299871 cites W2124751176 @default.
- W2037299871 cites W2126881800 @default.
- W2037299871 cites W2129084404 @default.
- W2037299871 cites W2132316084 @default.
- W2037299871 cites W2132737729 @default.