Matches in SemOpenAlex for { <https://semopenalex.org/work/W2037307974> ?p ?o ?g. }
- W2037307974 abstract "Previously, we have identified the Dictyostelium 7E gene promoter and shown that it is capable of driving expression in the same temporal and cAMP responsive manner as the endogenous gene during development. Furthermore, we have mapped the corresponding transcriptional regulatory sequences within the promoter. In the present study we used the lacZ reporter gene system to examine the role of 7E promoter elements in regulating cell-type specific expression during Dictyostelium morphogenesis. In situ detection of beta-galactosidase activity revealed that expression was induced within anterior prestalk cells at approximately 18 h of development. Subsequently, we found that promoter activity was independently regulated in subpopulations of prestalk cells. Element(s) upstream of position - 532 were necessary for expression in pstA cells while more proximal elements (located downstream of position - 426) were capable of directing expression in pstO cells. Deletion of a G-rich element ('GGT' box; 5'-GGT GAT GA-3') located between positions - 159 and - 152 resulted both in a loss of expression in pstA cells and aberrant expression in the prespore zone. Furthermore, the spatial organisation of reporter gene expression directed by this construct during culmination delineated a population of cells that have not been previously defined. These data suggest that the 7E gene is independently regulated in subpopulations of prestalk cells during development." @default.
- W2037307974 created "2016-06-24" @default.
- W2037307974 creator A5002222612 @default.
- W2037307974 creator A5034836695 @default.
- W2037307974 creator A5059195446 @default.
- W2037307974 creator A5091686423 @default.
- W2037307974 date "2001-08-01" @default.
- W2037307974 modified "2023-09-23" @default.
- W2037307974 title "Identification of novel elements which regulate the cell-type specificity of Dictyostelium 7E gene expression" @default.
- W2037307974 cites W104716945 @default.
- W2037307974 cites W155973773 @default.
- W2037307974 cites W1856768934 @default.
- W2037307974 cites W1862028121 @default.
- W2037307974 cites W1916172146 @default.
- W2037307974 cites W1953293010 @default.
- W2037307974 cites W1966179312 @default.
- W2037307974 cites W1966899407 @default.
- W2037307974 cites W1986465767 @default.
- W2037307974 cites W1986644535 @default.
- W2037307974 cites W1988176977 @default.
- W2037307974 cites W1989053289 @default.
- W2037307974 cites W1990741152 @default.
- W2037307974 cites W1992289621 @default.
- W2037307974 cites W2000612768 @default.
- W2037307974 cites W2004474008 @default.
- W2037307974 cites W2012219330 @default.
- W2037307974 cites W2024654045 @default.
- W2037307974 cites W2030337200 @default.
- W2037307974 cites W2034139344 @default.
- W2037307974 cites W2037243710 @default.
- W2037307974 cites W2044998145 @default.
- W2037307974 cites W2046123933 @default.
- W2037307974 cites W2050781353 @default.
- W2037307974 cites W2056625430 @default.
- W2037307974 cites W2069125601 @default.
- W2037307974 cites W2070850056 @default.
- W2037307974 cites W2088858357 @default.
- W2037307974 cites W2118516988 @default.
- W2037307974 cites W2119671728 @default.
- W2037307974 cites W2145518418 @default.
- W2037307974 cites W2147012124 @default.
- W2037307974 cites W2154917200 @default.
- W2037307974 cites W2162633841 @default.
- W2037307974 cites W2182503528 @default.
- W2037307974 cites W2185324773 @default.
- W2037307974 cites W2185471410 @default.
- W2037307974 cites W2339051255 @default.
- W2037307974 cites W2471927801 @default.
- W2037307974 cites W4232534952 @default.
- W2037307974 cites W4256274280 @default.
- W2037307974 cites W87952807 @default.
- W2037307974 cites W1967844217 @default.
- W2037307974 cites W2289014684 @default.
- W2037307974 doi "https://doi.org/10.1046/j.1432-0436.2001.068001022.x" @default.
- W2037307974 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11683490" @default.
- W2037307974 hasPublicationYear "2001" @default.
- W2037307974 type Work @default.
- W2037307974 sameAs 2037307974 @default.
- W2037307974 citedByCount "7" @default.
- W2037307974 countsByYear W20373079742016 @default.
- W2037307974 crossrefType "journal-article" @default.
- W2037307974 hasAuthorship W2037307974A5002222612 @default.
- W2037307974 hasAuthorship W2037307974A5034836695 @default.
- W2037307974 hasAuthorship W2037307974A5059195446 @default.
- W2037307974 hasAuthorship W2037307974A5091686423 @default.
- W2037307974 hasConcept C101762097 @default.
- W2037307974 hasConcept C104317684 @default.
- W2037307974 hasConcept C144024400 @default.
- W2037307974 hasConcept C1491633281 @default.
- W2037307974 hasConcept C149923435 @default.
- W2037307974 hasConcept C150194340 @default.
- W2037307974 hasConcept C161733203 @default.
- W2037307974 hasConcept C165864922 @default.
- W2037307974 hasConcept C170344550 @default.
- W2037307974 hasConcept C189014844 @default.
- W2037307974 hasConcept C2775889303 @default.
- W2037307974 hasConcept C2908647359 @default.
- W2037307974 hasConcept C40692019 @default.
- W2037307974 hasConcept C54355233 @default.
- W2037307974 hasConcept C86803240 @default.
- W2037307974 hasConcept C95444343 @default.
- W2037307974 hasConceptScore W2037307974C101762097 @default.
- W2037307974 hasConceptScore W2037307974C104317684 @default.
- W2037307974 hasConceptScore W2037307974C144024400 @default.
- W2037307974 hasConceptScore W2037307974C1491633281 @default.
- W2037307974 hasConceptScore W2037307974C149923435 @default.
- W2037307974 hasConceptScore W2037307974C150194340 @default.
- W2037307974 hasConceptScore W2037307974C161733203 @default.
- W2037307974 hasConceptScore W2037307974C165864922 @default.
- W2037307974 hasConceptScore W2037307974C170344550 @default.
- W2037307974 hasConceptScore W2037307974C189014844 @default.
- W2037307974 hasConceptScore W2037307974C2775889303 @default.
- W2037307974 hasConceptScore W2037307974C2908647359 @default.
- W2037307974 hasConceptScore W2037307974C40692019 @default.
- W2037307974 hasConceptScore W2037307974C54355233 @default.
- W2037307974 hasConceptScore W2037307974C86803240 @default.
- W2037307974 hasConceptScore W2037307974C95444343 @default.
- W2037307974 hasLocation W20373079741 @default.
- W2037307974 hasLocation W20373079742 @default.
- W2037307974 hasOpenAccess W2037307974 @default.