FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2037361537> ?p ?o ?g. }

• W2037361537 endingPage "2080" @default.
• W2037361537 startingPage "2071" @default.
• W2037361537 abstract "ABSTRACT Large-scale and in-depth characterization of the intestinal microbiota necessitates application of high-throughput 16S rRNA gene-based technologies, such as barcoded pyrosequencing and phylogenetic microarray analysis. In this study, the two techniques were compared and contrasted for analysis of the bacterial composition in three fecal and three small intestinal samples from human individuals. As PCR remains a crucial step in sample preparation for both techniques, different forward primers were used for amplification to assess their impact on microbial profiling results. An average of 7,944 pyrosequences, spanning the V1 and V2 region of 16S rRNA genes, was obtained per sample. Although primer choice in barcoded pyrosequencing did not affect species richness and diversity estimates, detection of Actinobacteria strongly depended on the selected primer. Microbial profiles obtained by pyrosequencing and phylogenetic microarray analysis (HITChip) correlated strongly for fecal and ileal lumen samples but were less concordant for ileostomy effluent. Quantitative PCR was employed to investigate the deviations in profiling between pyrosequencing and HITChip analysis. Since cloning and sequencing of random 16S rRNA genes from ileostomy effluent confirmed the presence of novel intestinal phylotypes detected by pyrosequencing, especially those belonging to the Veillonella group, the divergence between pyrosequencing and the HITChip is likely due to the relatively low number of available 16S rRNA gene sequences of small intestinal origin in the DNA databases that were used for HITChip probe design. Overall, this study demonstrated that equivalent biological conclusions are obtained by high-throughput profiling of microbial communities, independent of technology or primer choice." @default.
• W2037361537 created "2016-06-24" @default.
• W2037361537 creator A5025526643 @default.
• W2037361537 creator A5054572740 @default.
• W2037361537 creator A5057074180 @default.
• W2037361537 creator A5080388447 @default.
• W2037361537 date "2011-03-15" @default.
• W2037361537 modified "2023-10-18" @default.
• W2037361537 title "Microarray Analysis and Barcoded Pyrosequencing Provide Consistent Microbial Profiles Depending on the Source of Human Intestinal Samples" @default.
• W2037361537 cites W1964645494 @default.
• W2037361537 cites W1980750600 @default.
• W2037361537 cites W1987992874 @default.
• W2037361537 cites W2000987159 @default.
• W2037361537 cites W2002941870 @default.
• W2037361537 cites W2005021548 @default.
• W2037361537 cites W2012854889 @default.
• W2037361537 cites W2025986883 @default.
• W2037361537 cites W2036647435 @default.
• W2037361537 cites W2051322058 @default.
• W2037361537 cites W2059508477 @default.
• W2037361537 cites W2061559511 @default.
• W2037361537 cites W2063419767 @default.
• W2037361537 cites W2063984303 @default.
• W2037361537 cites W2064410624 @default.
• W2037361537 cites W2066452478 @default.
• W2037361537 cites W2072315314 @default.
• W2037361537 cites W2073548709 @default.
• W2037361537 cites W2080283023 @default.
• W2037361537 cites W2091922579 @default.
• W2037361537 cites W2095132285 @default.
• W2037361537 cites W2096566061 @default.
• W2037361537 cites W2098293009 @default.
• W2037361537 cites W2099890886 @default.
• W2037361537 cites W2107974372 @default.
• W2037361537 cites W2108166985 @default.
• W2037361537 cites W2111458006 @default.
• W2037361537 cites W2118087354 @default.
• W2037361537 cites W2128114769 @default.
• W2037361537 cites W2130077037 @default.
• W2037361537 cites W2130329894 @default.
• W2037361537 cites W2130802610 @default.
• W2037361537 cites W2143566426 @default.
• W2037361537 cites W2146178402 @default.
• W2037361537 cites W2149573313 @default.
• W2037361537 cites W2149824014 @default.
• W2037361537 cites W2152885278 @default.
• W2037361537 cites W2155066423 @default.
• W2037361537 cites W2155806125 @default.
• W2037361537 cites W2159480438 @default.
• W2037361537 cites W2161713232 @default.
• W2037361537 cites W2164095252 @default.
• W2037361537 cites W2165003751 @default.
• W2037361537 cites W2165829105 @default.
• W2037361537 cites W2165924947 @default.
• W2037361537 cites W2167617868 @default.
• W2037361537 cites W2167730457 @default.
• W2037361537 cites W2169330513 @default.
• W2037361537 doi "https://doi.org/10.1128/aem.02477-10" @default.
• W2037361537 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3067328" @default.
• W2037361537 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21257804" @default.
• W2037361537 hasPublicationYear "2011" @default.
• W2037361537 type Work @default.
• W2037361537 sameAs 2037361537 @default.
• W2037361537 citedByCount "147" @default.
• W2037361537 countsByYear W20373615372012 @default.
• W2037361537 countsByYear W20373615372013 @default.
• W2037361537 countsByYear W20373615372014 @default.
• W2037361537 countsByYear W20373615372015 @default.
• W2037361537 countsByYear W20373615372016 @default.
• W2037361537 countsByYear W20373615372017 @default.
• W2037361537 countsByYear W20373615372018 @default.
• W2037361537 countsByYear W20373615372019 @default.
• W2037361537 countsByYear W20373615372020 @default.
• W2037361537 countsByYear W20373615372021 @default.
• W2037361537 countsByYear W20373615372022 @default.
• W2037361537 countsByYear W20373615372023 @default.
• W2037361537 crossrefType "journal-article" @default.
• W2037361537 hasAuthorship W2037361537A5025526643 @default.
• W2037361537 hasAuthorship W2037361537A5054572740 @default.
• W2037361537 hasAuthorship W2037361537A5057074180 @default.
• W2037361537 hasAuthorship W2037361537A5080388447 @default.
• W2037361537 hasBestOaLocation W20373615371 @default.
• W2037361537 hasConcept C104317684 @default.
• W2037361537 hasConcept C125009961 @default.
• W2037361537 hasConcept C143121216 @default.
• W2037361537 hasConcept C150194340 @default.
• W2037361537 hasConcept C15151743 @default.
• W2037361537 hasConcept C174160445 @default.
• W2037361537 hasConcept C178790620 @default.
• W2037361537 hasConcept C185592680 @default.
• W2037361537 hasConcept C2777563447 @default.
• W2037361537 hasConcept C2778169975 @default.
• W2037361537 hasConcept C42062724 @default.
• W2037361537 hasConcept C54355233 @default.
• W2037361537 hasConcept C70721500 @default.
• W2037361537 hasConcept C86803240 @default.
• W2037361537 hasConcept C89423630 @default.
• W2037361537 hasConcept C95371953 @default.

• next