FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2037374013> ?p ?o ?g. }

• W2037374013 endingPage "2892" @default.
• W2037374013 startingPage "2886" @default.
• W2037374013 abstract "Article17 June 2002free access Crystal structure of vesicular stomatitis virus matrix protein Martin Gaudier Martin Gaudier Laboratoire d'Enzymologie et Biochimie Structurales, CNRS, 91198 Gif sur Yvette, Cedex, France Search for more papers by this author Yves Gaudin Yves Gaudin Laboratoire de Génétique des Virus, CNRS, 91198 Gif sur Yvette, Cedex, France Search for more papers by this author Marcel Knossow Corresponding Author Marcel Knossow Laboratoire d'Enzymologie et Biochimie Structurales, CNRS, 91198 Gif sur Yvette, Cedex, France Search for more papers by this author Martin Gaudier Martin Gaudier Laboratoire d'Enzymologie et Biochimie Structurales, CNRS, 91198 Gif sur Yvette, Cedex, France Search for more papers by this author Yves Gaudin Yves Gaudin Laboratoire de Génétique des Virus, CNRS, 91198 Gif sur Yvette, Cedex, France Search for more papers by this author Marcel Knossow Corresponding Author Marcel Knossow Laboratoire d'Enzymologie et Biochimie Structurales, CNRS, 91198 Gif sur Yvette, Cedex, France Search for more papers by this author Author Information Martin Gaudier1, Yves Gaudin2 and Marcel Knossow 1 1Laboratoire d'Enzymologie et Biochimie Structurales, CNRS, 91198 Gif sur Yvette, Cedex, France 2Laboratoire de Génétique des Virus, CNRS, 91198 Gif sur Yvette, Cedex, France *Corresponding author. E-mail: [email protected] The EMBO Journal (2002)21:2886-2892https://doi.org/10.1093/emboj/cdf284 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The vesicular stomatitis virus (VSV) matrix protein (M) interacts with cellular membranes, self-associates and plays a major role in virus assembly and budding. We present the crystallographic structure, determined at 1.96 Å resolution, of a soluble thermolysin resistant core of VSV M. The fold is a new fold shared by the other vesiculovirus matrix proteins. The structure accounts for the loss of stability of M temperature-sensitive mutants deficient in budding, and reveals a flexible loop protruding from the globular core that is important for self-assembly. Membrane floatation shows that, together with the M lysine-rich N-terminal peptide, a second domain of the protein is involved in membrane binding. Indeed, the structure reveals a hydrophobic surface located close to the hydrophobic loop and surrounded by conserved basic residues that may constitute this domain. Lastly, comparison of the negative-stranded virus matrix proteins with retrovirus Gag proteins suggests that the flexible link between their major membrane binding domain and the rest of the structure is a common feature shared by these proteins involved in budding and virus assembly. Introduction Enveloped viruses acquire their membranes by budding from host cell membranes. One viral protein, the matrix protein, plays a major role in budding of negative-stranded viruses, including orthomyxoviruses (Gomez-Puertas et al., 2000), filoviruses (Timmins et al., 2001) or rhabdoviruses (Flamand, 1970; Mebatsion et al., 1999). Vesicular stomatitis virus (VSV) has long served as a model for studies of rhabdovirus budding. VSV is an enveloped virus with a single-stranded non-segmented RNA genome of negative polarity coding for five proteins; its genome is associated with the nucleoprotein (N), the large protein (L) and the phosphoprotein (P) to form the nucleocapsid. Together, L and P proteins form the RNA-dependent RNA polymerase involved in the transcription of the genome in viral mRNAs, and in its replication. The matrix protein (M) is involved in later steps of the infectious cycle. It condenses the nucleocapsid into a tightly coiled helical structure (Newcomb and Brown, 1981; Newcomb et al., 1982) and, probably as a consequence, inhibits the transcription of the viral genome (Clinton et al., 1978; Martinet et al., 1979). This tightly coiled structure seems to be formed near cellular membranes where M is bound (Odenwald et al., 1986; Flood and Lyles, 1999), and gets enveloped during budding by a lipid bilayer containing the viral glycoprotein. Strong evidence that M plays a major role in VSV budding comes from studies of VSV temperature-sensitive (ts) mutants affected in budding that were shown to be mutated in the M gene and were complemented by wild-type plasmid-derived M (Flamand, 1970; Lyles et al., 1996). M is a small 229 amino acid protein (26.6 kDa) com prising a positively charged N-terminal part (there are eight lysine residues in the first 20 residues). The function of M is not limited to viral assembly and budding. M expression causes the rounding of cells, a phenotype characteristic of VSV infection (Blondel et al., 1990; Melki et al., 1994). M also enhances the availability of the cellular translation machinery by inhibiting the export of RNAs from the nucleus (Her et al., 1997; Petersen et al., 2000; von Kobbe et al., 2000). In Xenopus oocytes, this inhibition is triggered by the interaction of M with nuclear pores (Petersen et al., 2000; von Kobbe et al., 2000) and may be related to M inhibition of host cell transcription (Black and Lyles, 1992; Black et al., 1994). Two biochemical properties of VSV M have been studied: auto-association and membrane binding. VSV M self-associates into large multimers at physiological NaCl concentration (McCreedy et al., 1990), a process initiated by trypsin-sensitive nucleation sites. Increasing the salt concentration reverses this self-association (Gaudin et al., 1995). M self-association could be important for nucleocapsid compaction and may constitute a driving force for budding. VSV M cell membrane association is also necessary for budding (Lenard, 1996). This interaction was proven to be extremely stable in vivo as it is not destabilized by harsh treatments such as 2 M KCl or pH 11 (Bergmann and Fusco, 1988; Chong and Rose, 1993, 1994). The regions of the protein involved in membrane association are not completely defined: hydrophobic photolabelling using [125I]TID has identified the N-terminal region of the protein (Lenard and Vanderoef, 1990), but M mutants deleted from the N-terminal domain still interact with cellular membranes, suggesting that another domain of the protein is involved (Chong and Rose, 1994; Ye et al., 1994). Due to its tendency to self-associate, M could not be crystallized. In an attempt to isolate a fragment of VSV M for crystallization, we had shown previously that trypsin-cleaved M [Mt, residues 44–229 (Ogden et al., 1986)] is recruited in M aggregates, but does not aggregate on its own, and thermolysin-cleaved M (Mth) neither self-associates nor is recruited in aggregates (Figure 1A; Gaudier et al., 2001). Here we report the three-dimensional structure of the Mth of VSV Indiana (Orsay strain), which is the first structure of a rhabdovirus protein to be determined. The Mth fold is a new fold. The structure shows that M of VSV ts mutants deficient in budding is destabilized with respect to the wild type, which accounts for their phenotype. The structure also enables us to identify the regions of M involved in its interaction with membranes. Comparison to the structures and domain organization of other negative-stranded virus matrix proteins and of retrovirus Gag proteins suggests that these viruses employ a similar strategy for the membrane targeting part of the budding process. Figure 1.(A) Schematic of VSV M sequence. Mt and Mth are trypsin and thermolysin digestion products of M, respectively; Mth is the species whose structure was determined crystallographically. (B) Ribbon diagram of VSV M. Rainbow colouring, from blue to red, describes the N-terminal to C-terminal direction of the polypeptide chain. The interruption of the chain in the large five-stranded β-sheet corresponds to the thermolysin cleavage in the 121–128 loop that connects the second strand to the third. (C) Topology diagram of VSV M. β-strands are represented as arrows and α-helices as rectangles. Colour code is as in (A). This figure and Figure 3 were drawn with MOLSCRIPT (Kraulis, 1991) and Raster3D (Merritt and Bacon, 1997). Download figure Download PowerPoint Results and discussion Structure determination and description M purified from virus grown on BHK cells was cleaved by thermolysin to obtain a resistant core Mth. This core, composed of two associated fragments [48–121 and 122 (or 123 or 124, depending on the thermolysin cleavage site) −229], was crystallized (Gaudier et al., 2001) and the structure was determined by the single isomorphous replacement with anomalous scattering method using a native crystal and a mercury derivative (data and phasing statistics are shown in Table I). The experimental maps calculated after SHARP and DM at 1.96 Å resolution enabled us to trace most of the polypeptide chain. This made it clear that the single mercury atom per asymmetric unit is not bound to the single M cysteine residue, but close to histidine 143 and threonine 80; this is due to an oxidation of the M single cysteine residue to cysteic acid, which makes this residue unavailable for complexation with methyl mercury, as shown by analysis of difference electron density maps following refinement. The refined model comprises M residues 58–121 and 128–227, and 115 water molecules; the crystallographic R factor is 20.6% (free R factor, 24%) at 1.96 Å resolution. Co ordinates have been deposited with the Protein Data Bank (PDB; accession code 1LG7). Table 1. Crystallographic statistics Data collection Native MeHgCl Wavelength (Å) 1.00 0.98 Resolution (Å) 1.96 (2.01) 2.6 (2.66) Reflections 209 741 79 001 Unique reflections 12 771 9425 Completeness (%) 99.6 (95.3) 91.8 (88.7) Rsym (%) 6.9 (41) 6.2 (18.3) I/σ 30 (3.5) 11.6 (3.9) Phasing Riso (%) 13 Phasing power RCullis FOM (ano) 1.27 (iso) 2.25 (ano) 0.88 (iso) 0.63 acentric 0.48 centric 0.40 Refinement No. of reflections in working set 12 032 Rcryst (%) 20.6 (23.9) Rfree (%) 24.1 (32.7) Average B (Å2) 29.7 Residues 164 (out of 182) Bond length r.m.s.d. (Å) 0.005 Bond angles r.m.s.d. (°) 1.47 Water molecules 115 Mth is a single globular domain (Figure 1B and C) with dimensions 40 × 25 × 20 Å3. The N-terminal part is composed of a large five-stranded anti-parallel β-sheet (β1–β5) packed against two α-helices (α1 and α2); the C-terminal part comprises a small two-stranded anti-parallel β-sheet (β6 and β7) and an α-helix (α3). The N-terminal and C-terminal parts are connected by a 20 amino acid residue peptide without any defined secondary structure that links strand β5 to helix α3. We observed two kinds of interaction that give rise to a stable assembly of the N- and C-terminal parts of Mth: the three α-helices are packed together around a hydrophobic core, and strands β3 and β7 establish parallel β-sheet interactions. The cleavages by thermolysin between residues 121 and 124 take place in a surface loop delimited by prolines 120 and 129 that connects strands β2 and β3. This loop contains the hydrophobic peptide PAVLA that is involved in M self-assembly (Gaudier et al., 2001). Because of the multiple cleavage products of M due to the limited specificity of thermolysin, we could not determine whether residues 122–123 had been cleaved off the crystallized fragments or were present in the structure, but not sufficiently ordered to be visible in electron density maps. The matrix proteins of several vesiculoviruses [VSV—Orsay and New Jersey strains, Chandipura virus, Piry virus and spring viremia carp virus (SVCV)] have been sequenced; an alignment of these sequences is presented in Figure 2. These proteins have a low identity with the VSV Orsay strain (∼60, 40, 40 and 20%, respectively) but a significant similarity (∼80, 50, 50 and 50%, respectively), as previously reported (Barge et al., 1996; Taylor et al., 1999). Twelve positions of conserved residues are on the surface of M and the corresponding total area is <5% of the total accessible surface area of the Mth fragment, demonstrating a very low degree of conservation of M surface among vesiculoviruses. By contrast, most of the conserved residues (22 out of 34) are buried in the structure of the protein and their conservation is due to folding or stability constraints. This strongly suggests that the VSV M fold is also conserved amongst all vesiculovirus matrix proteins. Figure 2.Sequence alignment of the vesiculovirus Ms from strains Orsay (DDJB/EMBL/GenBank accession No. J02428_3), New Jersey (accession No. M14553_1), Chandipura (accession No. AF128868_2), Piry (accession No. D26175_2) and spring viremia carp virus (accession No. U18101_3) was performed with Clustal_W (Thompson et al., 1994). Conserved and similar residues visible in the structure are dark and light coloured, respectively. Blue, buried residues (side chain or glycine Cα accessible surface <10 Å2); red, surface residues (side chain or glycine Cα accessible surface >10 Å2). Download figure Download PowerPoint We have searched the PDB (Bernstein et al., 1977) for proteins that are structurally homologous to M, using the program DALI (Holm and Sander, 1993). No match of statistical significance was found, the best match being with a phospholipase a2 fragment (PDB accession code 1rlw), an Ig-like protein, and aligns only the five-stranded β-sheet of M. Therefore, the vesiculovirus matrix protein fold represents a new fold. M temperature-sensitive mutants VSV ts mutants have been extensively studied and classified in complementation groups. One of these groups is characterized by a default in budding: RNA and proteins are produced at normal levels, yet the production of viral particles is decreased ∼1000-fold at the non-permissive temperature (Flamand, 1970); these viruses are mutated in the M protein. Four of the mutations that have been characterized are located in the Mth core (Figure 3). One of these mutants, L111F, aggregates at the non-permissive temperature, as opposed to wild-type M or to a revertant (Lyles, 2000). This is accounted for by the structure; leucine 111 is surrounded by hydrophobic residues and there is no space to accommodate the aromatic ring of the mutant phenylalanine. Therefore, the structure of the ts mutant is expected to be less stable than that of wild-type M, as suggested by its biochemical behaviour. The structure suggests that two additional ts mutant M proteins have similar properties: mutation A133D substitutes a charged residue to an alanine located in a hydrophobic environment, and mutations F110S and K204T are substitutions that introduce hydrophilic groups in the hydrophobic environments of the phenylalanine side chain and the aliphatic part of the lysine side chain. Three of the four residues substituted in the three mutants listed so far are in the interior of the protein (their accessible surface is <10 Å2), so that a change at these positions is unlikely to affect directly the interaction of M with viral or cellular proteins; therefore, it is most likely that the ts phenotype of these three mutants is associated to a lower stability of M, as established in the case of the L111F substitution. Figure 3.VSV ts mutants in M that are deficient in budding. Each view is a close-up of the structure around a position where a ts mutation that confers budding deficiency to VSV has been identified; in each case the position of the mutation and the amino acid substitution are indicated, together with the most common designation of the mutant (in parentheses). Mutated residues (blue) and those that contact them (purple) are drawn as ball and stick models. Download figure Download PowerPoint The substitution of the fourth ts mutant located in the Mth core involves residues on the surface of the protein. The mutant TsG31, with a K→E substitution at position 215, disrupts a hydrogen bond between the central β-sheet and the C-terminal small two-stranded β-sheet of the protein. Residue 215 is not conserved among vesiculoviruses (it is changed to a serine or a glutamine in other members of this family of viruses) so that it is unlikely to be involved in an interaction with a cellular partner that vesiculoviruses would have in common. Since the K215E mutation destabilizes the interaction of the two parts of the Mth core, it is likely that, in this case too, the ts phenotype is due to M destabilization. Membrane interaction Membrane association of M has been demonstrated both in vitro (Zakowski et al., 1981) and in vivo (Chong and Rose, 1994). In order to define the regions of the structure that are involved in the interaction of M with phospholipids, we performed membrane floatation experiments. We used, on the one hand, negatively charged liposomes made with phosphatidylcholine and phosphatidylserine in identical quantities, meant to mimic the internal face of the plasma or VSV membranes (Zakowski et al., 1981), and, on the other hand, either M or the fragments of M that we have characterized, i.e. Mt and Mth cores (Figure 1A). Figure 4 summarizes our results. M binds strongly to liposomes (Figure 4A). This interaction is weakened in the presence of 700 mM NaCl (Figure 4B). This is consistent with previous data on M membrane association (Zakowski et al., 1981). Figure 4.Membrane floatation of M, Mt core and Mth core in the presence of PC:PS liposomes. Eight fractions were collected from each gradient and samples from each fraction were separated on SDS 14% PAGE and stained with Coomassie Blue. Download figure Download PowerPoint The interaction of Mt with liposomes (Figure 4C) is weaker than that of M, which is consistent with the proposal that the lysine rich N-terminal part of the protein makes a major contribution to binding to negatively charged liposomes, and to interaction with the viral membrane (Lenard and Vanderoef, 1990). As observed with M, the interaction of Mt with membranes is prevented in the presence of 700 mM NaCl, suggesting that this association also involves electrostatic interactions which, in M, come in addition to those of the N-terminal part. Membrane association of Mth is hardly detectable (Figure 4E). The main difference between Mth and Mt is a cut in the hydrophobic pentapeptide Pro120-Ala124, which is embedded in a disordered loop on the surface of the protein. The membrane floatation results with Mth strongly suggest that this loop is involved in the association of Mt with membranes. The Pro120-Ala124 pentapeptide might contribute to Mt (or M) membrane association either by a direct interaction with the bilayer or by aggregating Mt (or M) proteins (Gaudier et al., 2001) that, individually, would bind more weakly to liposomes. To summarize, three regions contribute to M binding to liposomes: its positively charged N-terminal part, which makes a major contribution, the 120–129 loop and, finally, a positively charged portion of Mth surface, which provides a smaller additional contribution. On the Mth surface, there are two main regions where the electrostatic potential is positive; the largest one is contributed by two arginine and three lysine residues, located at positions 76, 79, 117, 168 and 204, which surround the hydrophobic surface of residues Val84, Pro77, Ala118 and Tyr81 and are located close to the PAVLA hydrophobic peptide of the 120–129 loop (Figure 5). As discussed for a number of proteins (Bouma et al., 1999; Macedo-Ribeiro et al., 1999; Dessen et al., 2000), the combined presence of hydrophobic and basic residues suggests that this is a likely candidate for being the region responsible for Mth residual interaction with membranes. However, further studies are necessary to locate the exact membrane targeting sequence in Mth. Figure 5.Distribution of electrostatic potential on VSV M surface. Regions where the electrostatic potential is less than −10 kbT are red, while those where it is more than +10 kbT are blue (kb, Boltzmann constant; T, absolute temperature). A basic patch is clearly visible close to the PAVLA peptide and to a hydrophobic patch comprised of residues Val84, Pro77, Ala118 and Tyr81. This figure was drawn with GRASP (Nicholls et al., 1991). Download figure Download PowerPoint The structures of negative-stranded virus matrix proteins In addition to the structure of VSV M, crystal structures of domains of two negative single-stranded RNA virus matrix proteins have been determined: those of the influenza (Sha and Luo, 1997; Arzt et al., 2001) and Ebola viruses (Dessen et al., 2000). VSV M has been shown to play a pivotal role in viral assembly (Flamand, 1970; Lyles et al., 1996); the matrix proteins of the influenza or Ebola viruses are thought to do so, based, in particular, on the formation of particles that bud from cells transfected with the matrix protein alone (Gomez-Puertas et al., 2000; Timmins et al., 2001). As part of their function in virus assembly, these proteins bind to membranes or to negatively charged phospholipids (Zakowski et al., 1981; Zhang and Lamb, 1996; Ruigrok et al., 2000; Baudin et al., 2001). In addition to their involvement in budding, matrix proteins of negative single-stranded RNA viruses have functions that differ; for example, influenza M1 shuttles between the nucleus and the cytoplasm and is required for ribonucleoprotein transport from the nucleus (Bui et al., 2000), whereas VSV M inhibits the export of RNAs from the nucleus by binding to nuclear pores (Her et al., 1997; von Kobbe et al., 2000). Although their common biochemical properties and functions had led to the proposal that the negative-stranded RNA virus matrix proteins belong to the same family (Lenard, 1996), the sequences and folds of the domains of the three negative-stranded RNA virus matrix proteins whose structure has been determined differ. The difference of Ebola virus VP40 and VSV M folds is particularly intriguing because their genome organization suggests that rhabdoviruses and filoviruses share a common ancestor (Peters et al., 1996). Thus, the structural organization of negative-stranded RNA virus matrix proteins does not seem to be extensively constrained despite the functional similarities of these proteins. In particular, two components of the budding processes of negative-stranded RNA viruses, membrane association and the recruitment of cellular partners, share some features; as a consequence, the matrix proteins of negative-stranded RNA viruses have two characteristics in common. First, Ebola VP40 and VSV M share a PPXY motif that is known to be recognized by an E3 ubiquitin ligase (Harty et al., 2000, 2001); this motif has been shown to influence budding efficiency (Jayakar et al., 2000) through a mechanism that is shared by retroviruses (Craven et al., 1999) and still needs to be clarified. Secondly, all three matrix proteins self-associate and bind to membranes. VP40 forms defined oligomers (Scianimanico et al., 2000); VSV M and influenza M1 (Gomez-Puertas et al., 2000) form multimers or bind as multiple copies to the viral ribonucleoprotein complex. In all three cases, the sequences used for oligomerization or ribonucleoprotein binding are distinct from the major membrane binding domain (Kaptur et al., 1991; Scianimanico et al., 2000; Baudin et al., 2001). Furthermore, the structure of Ebola VP40 suggests that the link between its membrane binding and oligomerization domains is flexible (Scianimanico et al., 2000). Limited proteolysis results indicate that the link between the major membrane binding domain of VSV M (the N-terminal lysine-rich peptide) and its ribonucleoprotein binding domain (the Mt core) is also flexible (Kaptur et al., 1991); similar conclusions apply to influenza M1 (Baudin et al., 2001), and to the link between the capsid protein and the membrane targeting surface of MA in the Gag polyprotein of retroviruses (Matthews et al., 1996). This flexibility and the multiple copy number of these proteins allow, through an avidity effect, a strong membrane association of the virus core that will ultimately lead to particle budding. Materials and methods Data collection and processing Purification and crystallization of the Mth core of VSV Indiana (Orsay strain) have been reported elsewhere (Gaudier et al., 2001). Crystals are in space group P42(1)2 with unit cell dimensions a = b = 80.7 Å, c = 51.7 Å and one molecule per asymmetric unit. A single heavy atom derivative was obtained by soaking a native crystal in 1 mM MeHgCl, 30% PEG 2000 monomethylether, 200 mM AcNa and 20 mM phosphate pH 8.5 for 12 h at 18°C. Crystals were cryoprotected in harvesting buffer to which glycerol was added to a final 20% v:v ratio and kept frozen at 110 K for data collection. Data were collected at beamline BM30 of the European Synchrotron Radiation Facility (ESRF, Grenoble, France), using a MAR image plate detector. Native data were collected to 1.96 Å resolution at 1 Å. SIRAS data were collected up to 2.6 Å near the L III shell edge of mercury. Integration, scaling and merging of diffraction intensity measurements were performed with the programs Denzo and Scalepack (Otwinowsky and Minor, 1997). Phase determination, model building, refinement and analysis A single mercury site was identified with the program SOLVE (Terwilliger and Berendzen, 1999) and further refinement was performed with the program SHARP (de la Fortelle and Bricogne, 1997). Density modification was performed with SOLOMON (Abrahams and Leslie, 1996), as implemented in SHARP, considering a solvent content of 40%. Sequence assignment and model building were performed manually with the program TURBO-FRODO (Roussel and Cambillau, 1991) after extension of the calculated phases from 2.6 to 1.96 Å with DM (Cowtan and Zhang, 1999). Atomic coordinates refinement was performed by alternating use of CNS (Brünger et al., 1998) with rounds of manual rebuilding. The final model includes 164 residues (58–121 and 128–227) and 118 water molecules. In vitro membrane association A total of 1 mg of phosphatidylserine (PS) and 1 mg of phosphatidylcholine (PC) dissolved in organic solvents were dried in vacuo. The lipid film was suspended in 1 ml of 150 mM NaCl and 20 mM Tris-HCl pH 7.5, and the mixture was bath-sonicated for 20 min. One hundred micrograms of M, its Mt core or its Mth core were incubated with 200 μg of liposomes at room temperature for 30 min. The final volume was 500 μl. After addition of the protein, NaCl concentration was either 150 or 700 mM. The volume of the mix was adjusted to 1 ml, 80% sucrose in 20 mM Tris-HCl pH 7.5 and 150 or 700 mM NaCl, overlaid with 3 ml of 60% sucrose and 1 ml of 10% sucrose either in 150 or 700 mM NaCl, and 20 mM Tris-HCl pH 7.5. Gradients were spun overnight at 50 000 r.p.m. in a SW55 rotor. Six hundred microlitre fractions were collected, out of which 30 μl samples were analyzed by SDS-PAGE. Two types of experiment were performed: low salt, where proteins and liposomes were incubated at 150 mM NaCl and spun in 150 mM NaCl gradients, and high salt, where proteins and liposomes were incubated at 700 mM NaCl then spun in 700 mM NaCl gradients. Acknowledgements We thank Anne Flamand for support, Christine Maheu for the production of VSV and Alain Roussel for initial advice on model building. We gratefully acknowledge Richard Kahn's help with the use of the BM30 beamline at ESRF. This work is supported by CNRS (UPR 9053 and UPR 9063) and by the ’Programme de Recherche Fondamentale en Microbiologie, Maladies Infectieuses et Parasitaires'. References Abrahams JP and Leslie AGW (1996) Methods used in the structure determination of bovine mitochondrial F1 ATPase. Acta Crystallogr D, 52, 30–42.Wiley Online LibraryCASPubMedWeb of Science®Google Scholar Arzt S, Baudin F, Barge A, Timmins P, Burmeister WP and Ruigrok RW (2001) Combined results from solution studies on intact influenza virus M1 protein and from a new crystal form of its N-terminal domain show that M1 is an elongated monomer. Virology, 279, 439–446.CrossrefCASPubMedWeb of Science®Google Scholar Barge A, Gagnon J, Chaffotte A, Timmins P, Langowsky J, Ruigrok R and Gaudin Y (1996) Rod-like shape of vesicular stomatitis virus matrix protein. Virology, 219, 465–470.CrossrefCASPubMedWeb of Science®Google Scholar Baudin F, Petit I, Weissenhorn W and Ruigrok RW (2001) In vitro dissection of the membrane and RNP binding activities of influenza virus M1 protein. Virology, 281, 102–108.CrossrefCASPubMedWeb of Science®Google Scholar Bergmann J and Fusco P (1988) The M protein of vesicular stomatitis virus associates specifically with the basolateral membranes of polarized epithelial cells independently of the G protein. J Cell Biol, 107, 1707–1715.CrossrefCASPubMedWeb of Science®Google Scholar Bernstein FC, Koetzle TF, Williams JGB, Meyer EF, Brice MD, Rodgers JR, Kennard O, Shimanouchi T and Tasumi M (1977) The Protein Data Bank: a computer-based archival file for macromolecular structures. J Mol Biol, 112, 535–542.CrossrefCASPubMedWeb of Science®Google Scholar Black B and Lyles D (1992) Vesicular stomatitis virus matrix protein inhibits host cell-directed transcription of target genes in vivo. J Virol, 66, 4058–4064.CrossrefCASPubMedWeb of Science®Google Scholar Black B, Brewer G and Lyles D (1994) Effect of vesicular stomatits virus matrix protein on host-directed translation in vivo. J Virol, 68, 555–560.CrossrefCASPubMedWeb of Science®Google Scholar Blondel D, Harmison G and Schubert M (1990) Role of matrix protein in cytopatho" @default.
• W2037374013 created "2016-06-24" @default.
• W2037374013 creator A5063712277 @default.
• W2037374013 date "2002-06-17" @default.
• W2037374013 modified "2023-09-25" @default.
• W2037374013 title "Crystal structure of vesicular stomatitis virus matrix protein" @default.
• W2037374013 cites W11473009 @default.
• W2037374013 cites W1264989886 @default.
• W2037374013 cites W1494199408 @default.
• W2037374013 cites W1516548724 @default.
• W2037374013 cites W1539796472 @default.
• W2037374013 cites W1546594087 @default.
• W2037374013 cites W1583235059 @default.
• W2037374013 cites W1584375422 @default.
• W2037374013 cites W1591680814 @default.
• W2037374013 cites W1620485937 @default.
• W2037374013 cites W1795605257 @default.
• W2037374013 cites W1913994512 @default.
• W2037374013 cites W1920895567 @default.
• W2037374013 cites W1935523902 @default.
• W2037374013 cites W1936207504 @default.
• W2037374013 cites W1965277349 @default.
• W2037374013 cites W1966041739 @default.
• W2037374013 cites W1968138925 @default.
• W2037374013 cites W1991503681 @default.
• W2037374013 cites W1992712618 @default.
• W2037374013 cites W1995017064 @default.
• W2037374013 cites W2000632863 @default.
• W2037374013 cites W2002815902 @default.
• W2037374013 cites W2006546036 @default.
• W2037374013 cites W2006838403 @default.
• W2037374013 cites W2008057478 @default.
• W2037374013 cites W2008316672 @default.
• W2037374013 cites W2022058405 @default.
• W2037374013 cites W2024313766 @default.
• W2037374013 cites W2026488805 @default.
• W2037374013 cites W2029574367 @default.
• W2037374013 cites W2032014779 @default.
• W2037374013 cites W2040705986 @default.
• W2037374013 cites W2046182783 @default.
• W2037374013 cites W2050499016 @default.
• W2037374013 cites W2056993875 @default.
• W2037374013 cites W2057397494 @default.
• W2037374013 cites W2065886385 @default.
• W2037374013 cites W2067812760 @default.
• W2037374013 cites W2071607445 @default.
• W2037374013 cites W2079127176 @default.
• W2037374013 cites W2080528351 @default.
• W2037374013 cites W2081229259 @default.
• W2037374013 cites W2094741369 @default.
• W2037374013 cites W2101446086 @default.
• W2037374013 cites W2106315897 @default.
• W2037374013 cites W2106882534 @default.
• W2037374013 cites W2109310563 @default.
• W2037374013 cites W2110687223 @default.
• W2037374013 cites W2111152104 @default.
• W2037374013 cites W2115188315 @default.
• W2037374013 cites W2117845552 @default.
• W2037374013 cites W2121443084 @default.
• W2037374013 cites W2125922177 @default.
• W2037374013 cites W2126051114 @default.
• W2037374013 cites W2142549149 @default.
• W2037374013 cites W2145722317 @default.
• W2037374013 cites W2147334926 @default.
• W2037374013 cites W2156633263 @default.
• W2037374013 cites W2157469282 @default.
• W2037374013 doi "https://doi.org/10.1093/emboj/cdf284" @default.
• W2037374013 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/126044" @default.
• W2037374013 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12065402" @default.
• W2037374013 hasPublicationYear "2002" @default.
• W2037374013 type Work @default.
• W2037374013 sameAs 2037374013 @default.
• W2037374013 citedByCount "86" @default.
• W2037374013 countsByYear W20373740132012 @default.
• W2037374013 countsByYear W20373740132013 @default.
• W2037374013 countsByYear W20373740132014 @default.
• W2037374013 countsByYear W20373740132015 @default.
• W2037374013 countsByYear W20373740132016 @default.
• W2037374013 countsByYear W20373740132017 @default.
• W2037374013 countsByYear W20373740132018 @default.
• W2037374013 countsByYear W20373740132019 @default.
• W2037374013 countsByYear W20373740132020 @default.
• W2037374013 countsByYear W20373740132021 @default.
• W2037374013 countsByYear W20373740132022 @default.
• W2037374013 crossrefType "journal-article" @default.
• W2037374013 hasAuthorship W2037374013A5063712277 @default.
• W2037374013 hasBestOaLocation W20373740132 @default.
• W2037374013 hasConcept C106487976 @default.
• W2037374013 hasConcept C159047783 @default.
• W2037374013 hasConcept C159985019 @default.
• W2037374013 hasConcept C192562407 @default.
• W2037374013 hasConcept C2522874641 @default.
• W2037374013 hasConcept C2779057330 @default.
• W2037374013 hasConcept C2909090456 @default.
• W2037374013 hasConcept C68434959 @default.
• W2037374013 hasConcept C86803240 @default.
• W2037374013 hasConceptScore W2037374013C106487976 @default.
• W2037374013 hasConceptScore W2037374013C159047783 @default.

• next