FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2037381371> ?p ?o ?g. }

• W2037381371 abstract "Three mutants of horseradish peroxidase isoenzyme C (HRPC) have been constructed in which the conserved distal aromatic residue Phe(41) has been substituted by Trp, Val or Ala and the properties of the mutant proteins have been compared with that of the wild-type. The ferric and ferrous states have been studied by resonance Raman, electronic absorption and Fourier-transform infrared spectroscopies, together with their respective fluoride and CO complexes as probes for the integrity of the distal haem-pocket hydrogen-bonding network. The catalytic properties of the mutants, most notably the HRPC-mutant Phe(41)-->Trp (F41W) variant, were also affected. Structural modelling suggests that the bulky indole group of the F41W mutant blocks the distal cavity, inhibiting the binding of fluoride and CO to the haem iron, severely impairing the reaction of the enzyme with H(2)O(2) to form Compound I. Substitution with the smaller side-chain residues Val or Ala resulted in a 2-fold increase in the affinity of the mutants for the aromatic donor benzhydroxamic acid (BHA) compared with the wild-type, whereas the sterically hindered F41W mutant was not able to bind BHA at all. All the mutations studied increased the amount of a ferric six-coordinate aquo-high-spin species. On the other hand, the similarity in the Fe-Im stretching frequencies of the mutants and wild-type protein suggests that the distal haem-pocket mutations do not cause any substantive changes on the proximal side of the haem. Spectra of the HRPC mutant Phe(41)-->Ala-CO and the HRPC mutant Phe(41)-->Val-CO complexes strongly suggested a weakening of the interaction between CO and Arg(38) due to a secondary rearrangement of the haem relative to helix B. The effects observed for these HRP mutants were somewhat different from those noted recently for the analogous Coprinus cinereus peroxidase (CIP) mutants, particularly the Trp mutant. These differences can be reconciled in part as being due to the smaller size of the distal cavity of HRP compared with that of CIP." @default.
• W2037381371 created "2016-06-24" @default.
• W2037381371 creator A5011834580 @default.
• W2037381371 creator A5014389744 @default.
• W2037381371 creator A5044637051 @default.
• W2037381371 date "2002-05-01" @default.
• W2037381371 modified "2023-09-26" @default.
• W2037381371 title "Spectroscopic characterization of mutations at the Phe41 position in the distal haem pocket of horseradish peroxidase C: structural and functional consequences" @default.
• W2037381371 cites W1531817095 @default.
• W2037381371 cites W1547838161 @default.
• W2037381371 cites W1549928911 @default.
• W2037381371 cites W1575373997 @default.
• W2037381371 cites W1590103084 @default.
• W2037381371 cites W1606032544 @default.
• W2037381371 cites W1866116049 @default.
• W2037381371 cites W1965493010 @default.
• W2037381371 cites W1967008734 @default.
• W2037381371 cites W1968242010 @default.
• W2037381371 cites W1968364732 @default.
• W2037381371 cites W1970332353 @default.
• W2037381371 cites W1977718959 @default.
• W2037381371 cites W1978044046 @default.
• W2037381371 cites W1980727378 @default.
• W2037381371 cites W1984276256 @default.
• W2037381371 cites W1996617581 @default.
• W2037381371 cites W2002978594 @default.
• W2037381371 cites W2013253110 @default.
• W2037381371 cites W2016830482 @default.
• W2037381371 cites W2017147627 @default.
• W2037381371 cites W2023988692 @default.
• W2037381371 cites W2024117678 @default.
• W2037381371 cites W2033931593 @default.
• W2037381371 cites W2074297946 @default.
• W2037381371 cites W2075333740 @default.
• W2037381371 cites W2077699250 @default.
• W2037381371 cites W2078996434 @default.
• W2037381371 cites W2085266874 @default.
• W2037381371 cites W2086073464 @default.
• W2037381371 cites W2089132302 @default.
• W2037381371 cites W2092643557 @default.
• W2037381371 cites W2165898505 @default.
• W2037381371 cites W2179092050 @default.
• W2037381371 cites W2404128660 @default.
• W2037381371 doi "https://doi.org/10.1042/0264-6021:3630571" @default.
• W2037381371 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1222510" @default.
• W2037381371 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11964158" @default.
• W2037381371 hasPublicationYear "2002" @default.
• W2037381371 type Work @default.
• W2037381371 sameAs 2037381371 @default.
• W2037381371 citedByCount "9" @default.
• W2037381371 countsByYear W20373813712015 @default.
• W2037381371 countsByYear W20373813712023 @default.
• W2037381371 crossrefType "journal-article" @default.
• W2037381371 hasAuthorship W2037381371A5011834580 @default.
• W2037381371 hasAuthorship W2037381371A5014389744 @default.
• W2037381371 hasAuthorship W2037381371A5044637051 @default.
• W2037381371 hasBestOaLocation W20373813712 @default.
• W2037381371 hasConcept C104317684 @default.
• W2037381371 hasConcept C143065580 @default.
• W2037381371 hasConcept C162008176 @default.
• W2037381371 hasConcept C179104552 @default.
• W2037381371 hasConcept C181199279 @default.
• W2037381371 hasConcept C185592680 @default.
• W2037381371 hasConcept C192527728 @default.
• W2037381371 hasConcept C201194858 @default.
• W2037381371 hasConcept C207583985 @default.
• W2037381371 hasConcept C2776217839 @default.
• W2037381371 hasConcept C2777676563 @default.
• W2037381371 hasConcept C2780534640 @default.
• W2037381371 hasConcept C55493867 @default.
• W2037381371 hasConcept C71240020 @default.
• W2037381371 hasConcept C8010536 @default.
• W2037381371 hasConceptScore W2037381371C104317684 @default.
• W2037381371 hasConceptScore W2037381371C143065580 @default.
• W2037381371 hasConceptScore W2037381371C162008176 @default.
• W2037381371 hasConceptScore W2037381371C179104552 @default.
• W2037381371 hasConceptScore W2037381371C181199279 @default.
• W2037381371 hasConceptScore W2037381371C185592680 @default.
• W2037381371 hasConceptScore W2037381371C192527728 @default.
• W2037381371 hasConceptScore W2037381371C201194858 @default.
• W2037381371 hasConceptScore W2037381371C207583985 @default.
• W2037381371 hasConceptScore W2037381371C2776217839 @default.
• W2037381371 hasConceptScore W2037381371C2777676563 @default.
• W2037381371 hasConceptScore W2037381371C2780534640 @default.
• W2037381371 hasConceptScore W2037381371C55493867 @default.
• W2037381371 hasConceptScore W2037381371C71240020 @default.
• W2037381371 hasConceptScore W2037381371C8010536 @default.
• W2037381371 hasLocation W20373813711 @default.
• W2037381371 hasLocation W20373813712 @default.
• W2037381371 hasLocation W20373813713 @default.
• W2037381371 hasLocation W20373813714 @default.
• W2037381371 hasOpenAccess W2037381371 @default.
• W2037381371 hasPrimaryLocation W20373813711 @default.
• W2037381371 hasRelatedWork W1531270254 @default.
• W2037381371 hasRelatedWork W1554994168 @default.
• W2037381371 hasRelatedWork W173684358 @default.
• W2037381371 hasRelatedWork W1966287049 @default.
• W2037381371 hasRelatedWork W1969466983 @default.
• W2037381371 hasRelatedWork W1972746576 @default.
• W2037381371 hasRelatedWork W2001634917 @default.

• next