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- W2037400915 abstract "Single-strand conformation polymorphism (SSCP) analysis of the bipartite genomes of several UK isolates of barley yellow mosaic virus (BaYMV) was done using fragments of cDNA amplified by RT-PCR. Isolates differed in their SSCP patterns in several regions, but in no case was the pattern able to distinguish between common and r resistance-breaking strains. In regions where the nucleotide sequences of UK isolates had been determined, there was no simple relationship between numbers of nucleotide differences and SSCP patterns: differences of only 2 or 3 nucleotides (nt) gave different SSCP patterns, whereas differences of as many as 29 nt did not. Although SSCP analysis has some potential as a rapid and sensitive tool for distinguishing virus isolates, differences detected do not necessarily relate to biological properties and the results are highly dependent on gel conditions." @default.
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- W2037400915 date "1996-09-01" @default.
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- W2037400915 title "Single-strand conformation polymorphism analysis of RT-PCR products of UK isolates of barley yellow mosaic virus" @default.
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- W2037400915 doi "https://doi.org/10.1016/0168-1702(96)01328-7" @default.
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