Matches in SemOpenAlex for { <https://semopenalex.org/work/W2037441004> ?p ?o ?g. }
- W2037441004 endingPage "242" @default.
- W2037441004 startingPage "236" @default.
- W2037441004 abstract "Abstract Melatonin is a natural compound synthesized by a variety of organs. It has been described to possess cell protecting activity in normal cells but was shown to induce apoptotic cell death in cancer cells. We determined to which extent and based on which molecular mechanisms melatonin is able to cause apoptosis in HT‐29 human colon cancer cells. Induction of apoptosis was assessed by caspase‐3‐like activity, nuclear fragmentation and chromatin condensation. Melatonin, when given alone at a concentration of 1 mM, did not affect any of the apoptosis markers. It potentiated apoptosis induced by the flavonoid flavone significantly. Whereas flavone alone at a concentration of 150 μM led to a 8‐fold increase in caspase‐3‐like activity associated with around 40% of cells displaying DNA‐fragmentation, a combination of flavone and melatonin increased caspase‐3‐like activity 30‐fold and 80% of cells exhibited fragmentation of DNA when compared to untreated controls. Melatonin caused an increase in cytosolic lactate levels that most likely allows the flavone‐induced activation of the mitochondrial pyruvate/lactate importer to deliver more substrates to mitochondrial respiration. The subsequent increased production of mitochondrial O 2 − · in the presence of flavone was further increased by melatonin. Scavenging mitochondrial O 2 −. by benzoquinone or blocking the lactate/pyruvate transporter by 5‐nitro‐2‐(3‐phenylpropylamino) benzoate inhibited mitochondrial O 2 −. ‐generation and apoptosis execution mediated by flavone and melatonin. Our study provides evidence that melatonin potentiates flavone‐induced apoptosis in HT‐29 human colon cancer cells by enhancing the level of oxidizable substrates that can be transported into mitochondria in the presence of flavone. © 2005 Wiley‐Liss, Inc." @default.
- W2037441004 created "2016-06-24" @default.
- W2037441004 creator A5002421224 @default.
- W2037441004 creator A5025068207 @default.
- W2037441004 creator A5057449920 @default.
- W2037441004 date "2005-03-30" @default.
- W2037441004 modified "2023-10-15" @default.
- W2037441004 title "Melatonin potentiates flavone-induced apoptosis in human colon cancer cells by increasing the level of glycolytic end products" @default.
- W2037441004 cites W1564991199 @default.
- W2037441004 cites W1963671224 @default.
- W2037441004 cites W1966495770 @default.
- W2037441004 cites W1970017618 @default.
- W2037441004 cites W1970774549 @default.
- W2037441004 cites W1970991763 @default.
- W2037441004 cites W1971030675 @default.
- W2037441004 cites W1975085160 @default.
- W2037441004 cites W1975142555 @default.
- W2037441004 cites W1977318871 @default.
- W2037441004 cites W1981129901 @default.
- W2037441004 cites W1983838905 @default.
- W2037441004 cites W1997208169 @default.
- W2037441004 cites W2019578439 @default.
- W2037441004 cites W2023069349 @default.
- W2037441004 cites W2034269086 @default.
- W2037441004 cites W2048272556 @default.
- W2037441004 cites W2050918060 @default.
- W2037441004 cites W2058827994 @default.
- W2037441004 cites W2059234147 @default.
- W2037441004 cites W2065683945 @default.
- W2037441004 cites W2075035979 @default.
- W2037441004 cites W2077176956 @default.
- W2037441004 cites W2079663535 @default.
- W2037441004 cites W2081145901 @default.
- W2037441004 cites W2082728737 @default.
- W2037441004 cites W2082993541 @default.
- W2037441004 cites W2088965506 @default.
- W2037441004 cites W2092320337 @default.
- W2037441004 cites W2094302690 @default.
- W2037441004 cites W2094560884 @default.
- W2037441004 cites W2101979944 @default.
- W2037441004 cites W2141293774 @default.
- W2037441004 cites W2142482195 @default.
- W2037441004 cites W2142904884 @default.
- W2037441004 cites W2143427240 @default.
- W2037441004 cites W2155413339 @default.
- W2037441004 cites W2168897113 @default.
- W2037441004 cites W2254567115 @default.
- W2037441004 cites W3175140554 @default.
- W2037441004 doi "https://doi.org/10.1002/ijc.20837" @default.
- W2037441004 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15800915" @default.
- W2037441004 hasPublicationYear "2005" @default.
- W2037441004 type Work @default.
- W2037441004 sameAs 2037441004 @default.
- W2037441004 citedByCount "52" @default.
- W2037441004 countsByYear W20374410042012 @default.
- W2037441004 countsByYear W20374410042013 @default.
- W2037441004 countsByYear W20374410042014 @default.
- W2037441004 countsByYear W20374410042015 @default.
- W2037441004 countsByYear W20374410042016 @default.
- W2037441004 countsByYear W20374410042017 @default.
- W2037441004 countsByYear W20374410042018 @default.
- W2037441004 countsByYear W20374410042019 @default.
- W2037441004 countsByYear W20374410042020 @default.
- W2037441004 countsByYear W20374410042021 @default.
- W2037441004 countsByYear W20374410042022 @default.
- W2037441004 crossrefType "journal-article" @default.
- W2037441004 hasAuthorship W2037441004A5002421224 @default.
- W2037441004 hasAuthorship W2037441004A5025068207 @default.
- W2037441004 hasAuthorship W2037441004A5057449920 @default.
- W2037441004 hasBestOaLocation W20374410041 @default.
- W2037441004 hasConcept C134018914 @default.
- W2037441004 hasConcept C185592680 @default.
- W2037441004 hasConcept C18903297 @default.
- W2037441004 hasConcept C190283241 @default.
- W2037441004 hasConcept C191015642 @default.
- W2037441004 hasConcept C2778182776 @default.
- W2037441004 hasConcept C28859421 @default.
- W2037441004 hasConcept C31573885 @default.
- W2037441004 hasConcept C43759708 @default.
- W2037441004 hasConcept C55493867 @default.
- W2037441004 hasConcept C86803240 @default.
- W2037441004 hasConceptScore W2037441004C134018914 @default.
- W2037441004 hasConceptScore W2037441004C185592680 @default.
- W2037441004 hasConceptScore W2037441004C18903297 @default.
- W2037441004 hasConceptScore W2037441004C190283241 @default.
- W2037441004 hasConceptScore W2037441004C191015642 @default.
- W2037441004 hasConceptScore W2037441004C2778182776 @default.
- W2037441004 hasConceptScore W2037441004C28859421 @default.
- W2037441004 hasConceptScore W2037441004C31573885 @default.
- W2037441004 hasConceptScore W2037441004C43759708 @default.
- W2037441004 hasConceptScore W2037441004C55493867 @default.
- W2037441004 hasConceptScore W2037441004C86803240 @default.
- W2037441004 hasIssue "2" @default.
- W2037441004 hasLocation W20374410041 @default.
- W2037441004 hasLocation W20374410042 @default.
- W2037441004 hasOpenAccess W2037441004 @default.
- W2037441004 hasPrimaryLocation W20374410041 @default.
- W2037441004 hasRelatedWork W2035624349 @default.