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• W2037441963 abstract "The three-dimensional structure of a cyclic enterobacterial common antigen (ECA) having four trisaccharide repeating units has been investigated by NMR spectroscopy and molecular dynamics simulations. Three different NMR parameters were determined: (a) (1)H,(1)H cross-relaxation rates from NOE experiments were used for determination of proton-proton distances; (b) trans-glycosidic (3)J(C,H) scalar coupling constants analyzed via a Karplus-type relationship provided information on torsion angles; and (c) (1)H,(13)C one-bond dipolar couplings obtained in a dilute liquid-crystalline medium were interpreted in terms of the orientational order and molecular conformations. The molecular dynamics simulations of the dodecasaccharide were performed with explicit water and counterions, which are important factors that strongly influence molecular conformation. Subsequently, the results from computer simulation were used to generate a three-dimensional structure of the cyclic ECA which is consistent with the experimental NMR parameters." @default.
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• W2037441963 date "2001-02-28" @default.
• W2037441963 modified "2023-10-14" @default.
• W2037441963 title "Conformational Investigation of a Cyclic Enterobacterial Common Antigen Employing NMR Spectroscopy and Molecular Dynamics Simulations" @default.
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• W2037441963 doi "https://doi.org/10.1021/bi002282l" @default.
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