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- W2037450890 abstract "Significance Using a single, nonintegrating episome, containing an optimized assembly of reprogramming factors and positive/negative selection markers, we generated germ-line–competent induced pluripotent stem (iPS) cells. To ensure that the iPS cells were transgene-free (i.e., were independent of exogenous reprogramming factors to achieve and maintain their pluripotent ground state) required the inclusion on the episome more that the classical four ( POU5F1 / OCT4 , KLF4 , SOX2 , and cMYC ) reprogramming factors. Also critical for the transgene-free iPS cells exhibiting competency for germ-line transmission was the requirement for growth in 2i medium." @default.
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- W2037450890 date "2014-07-07" @default.
- W2037450890 modified "2023-10-01" @default.
- W2037450890 title "Efficient germ-line transmission obtained with transgene-free induced pluripotent stem cells" @default.
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- W2037450890 doi "https://doi.org/10.1073/pnas.1409933111" @default.
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