Matches in SemOpenAlex for { <https://semopenalex.org/work/W2037481532> ?p ?o ?g. }
- W2037481532 endingPage "139" @default.
- W2037481532 startingPage "133" @default.
- W2037481532 abstract "We have investigated the effect of Zn ions on proton-transfer reactions in cytochrome c oxidase. In the absence of Zn(2+) the transition from the peroxy (P(R)) to the ferryl (F) intermediate has a time constant of approximately 100 micros and it is associated with proton transfer from the bulk solution with an intrinsic time constant of <<100 micros, but rate limited by the P(R)-->F transition. While in the presence of 100 microM Zn(2+) the P(R)-->F transition was slowed by a factor of approximately 2, proton uptake from the bulk solution was impaired to a much greater extent. Instead, about two protons (one proton in the absence of Zn(2+)) were taken up during the next reaction step, i.e. the decay of F to the oxidized (O) enzyme with a time constant of approximately 2.5 ms. Thus, the results show that there is one proton available within the enzyme that can be used for oxygen reduction and confirm our previous observation that F can be formed without proton uptake from the bulk solution. No effect of Zn(2+) was observed with a mutant enzyme in which Asp(I-132), at the entry point of the D-pathway, was replaced by its non-protonatable analogue Asn. In addition, no effect of Zn(2+) was observed on the F-->O transition rate when measured in D(2)O, because in D(2)O, the transition is internally slowed to approximately 10 ms, which is already slower than with bound Zn(2+). Together with earlier results showing that both the P(R)-->F and F-->O transitions are associated with proton uptake through the D-pathway, the results from this study indicate that Zn(2+) binds to and blocks the entrance of the D-pathway." @default.
- W2037481532 created "2016-06-24" @default.
- W2037481532 creator A5017776844 @default.
- W2037481532 creator A5056829720 @default.
- W2037481532 creator A5073711394 @default.
- W2037481532 date "2002-09-01" @default.
- W2037481532 modified "2023-10-13" @default.
- W2037481532 title "Inhibition of proton transfer in cytochrome c oxidase by zinc ions: delayed proton uptake during oxygen reduction" @default.
- W2037481532 cites W1492893002 @default.
- W2037481532 cites W1564432135 @default.
- W2037481532 cites W1756287317 @default.
- W2037481532 cites W1967888351 @default.
- W2037481532 cites W1970143646 @default.
- W2037481532 cites W1974514572 @default.
- W2037481532 cites W1975766672 @default.
- W2037481532 cites W1982652982 @default.
- W2037481532 cites W1990909752 @default.
- W2037481532 cites W1994008677 @default.
- W2037481532 cites W1996591517 @default.
- W2037481532 cites W1999489764 @default.
- W2037481532 cites W2001784797 @default.
- W2037481532 cites W2006550844 @default.
- W2037481532 cites W2017920582 @default.
- W2037481532 cites W2020904305 @default.
- W2037481532 cites W2022829886 @default.
- W2037481532 cites W2029667189 @default.
- W2037481532 cites W2034452304 @default.
- W2037481532 cites W2035594105 @default.
- W2037481532 cites W2038361800 @default.
- W2037481532 cites W2047016674 @default.
- W2037481532 cites W2047561022 @default.
- W2037481532 cites W2051135932 @default.
- W2037481532 cites W2054155863 @default.
- W2037481532 cites W2062716800 @default.
- W2037481532 cites W2076452477 @default.
- W2037481532 cites W2079108392 @default.
- W2037481532 cites W2083092605 @default.
- W2037481532 cites W2085800822 @default.
- W2037481532 cites W2105199391 @default.
- W2037481532 cites W2116150569 @default.
- W2037481532 cites W2150264392 @default.
- W2037481532 cites W2162109874 @default.
- W2037481532 cites W2169473716 @default.
- W2037481532 cites W41182407 @default.
- W2037481532 doi "https://doi.org/10.1016/s0005-2728(02)00268-2" @default.
- W2037481532 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12206905" @default.
- W2037481532 hasPublicationYear "2002" @default.
- W2037481532 type Work @default.
- W2037481532 sameAs 2037481532 @default.
- W2037481532 citedByCount "34" @default.
- W2037481532 countsByYear W20374815322012 @default.
- W2037481532 countsByYear W20374815322013 @default.
- W2037481532 countsByYear W20374815322014 @default.
- W2037481532 countsByYear W20374815322016 @default.
- W2037481532 countsByYear W20374815322018 @default.
- W2037481532 countsByYear W20374815322019 @default.
- W2037481532 countsByYear W20374815322021 @default.
- W2037481532 countsByYear W20374815322022 @default.
- W2037481532 crossrefType "journal-article" @default.
- W2037481532 hasAuthorship W2037481532A5017776844 @default.
- W2037481532 hasAuthorship W2037481532A5056829720 @default.
- W2037481532 hasAuthorship W2037481532A5073711394 @default.
- W2037481532 hasBestOaLocation W20374815321 @default.
- W2037481532 hasConcept C113196181 @default.
- W2037481532 hasConcept C121332964 @default.
- W2037481532 hasConcept C123249941 @default.
- W2037481532 hasConcept C145148216 @default.
- W2037481532 hasConcept C148898269 @default.
- W2037481532 hasConcept C178790620 @default.
- W2037481532 hasConcept C179104552 @default.
- W2037481532 hasConcept C181199279 @default.
- W2037481532 hasConcept C185592680 @default.
- W2037481532 hasConcept C535196362 @default.
- W2037481532 hasConcept C540031477 @default.
- W2037481532 hasConcept C54516573 @default.
- W2037481532 hasConcept C55493867 @default.
- W2037481532 hasConcept C55904794 @default.
- W2037481532 hasConcept C62520636 @default.
- W2037481532 hasConcept C71240020 @default.
- W2037481532 hasConcept C8010536 @default.
- W2037481532 hasConcept C93391505 @default.
- W2037481532 hasConceptScore W2037481532C113196181 @default.
- W2037481532 hasConceptScore W2037481532C121332964 @default.
- W2037481532 hasConceptScore W2037481532C123249941 @default.
- W2037481532 hasConceptScore W2037481532C145148216 @default.
- W2037481532 hasConceptScore W2037481532C148898269 @default.
- W2037481532 hasConceptScore W2037481532C178790620 @default.
- W2037481532 hasConceptScore W2037481532C179104552 @default.
- W2037481532 hasConceptScore W2037481532C181199279 @default.
- W2037481532 hasConceptScore W2037481532C185592680 @default.
- W2037481532 hasConceptScore W2037481532C535196362 @default.
- W2037481532 hasConceptScore W2037481532C540031477 @default.
- W2037481532 hasConceptScore W2037481532C54516573 @default.
- W2037481532 hasConceptScore W2037481532C55493867 @default.
- W2037481532 hasConceptScore W2037481532C55904794 @default.
- W2037481532 hasConceptScore W2037481532C62520636 @default.
- W2037481532 hasConceptScore W2037481532C71240020 @default.
- W2037481532 hasConceptScore W2037481532C8010536 @default.