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- W2037535826 abstract "Blackleg, caused by Leptosphaeria maculans (Desm.) Ces. et de Not., is a major disease of oilseed rape ( Brassica napus L.) worldwide. Molecular markers would be useful tools to assist breeding for blackleg resistance. The objective of this study was (i) to map and characterize quantitative trait loci (QTL) for field blackleg resistance in doubled haploid (DH) and F 2:3 populations from the cross ‘Darmor’ (resistant) × ‘Samourai’ (susceptible) and (ii) to compare QTL with those previously identified in the cross ‘Darmor‐ bzh ’ × ‘Yudal’. A total of 134 DH lines and 185 F 2:3 families were genotyped with random amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) markers and assessed for a disease index of resistance in 1998 and/or 1999 in one location. Genetic maps derived from the two populations included a total of 257 and 81 markers, respectively. Up to 30% of these markers were common to the Darmor‐ bzh × Yudal map previously used. A total of six and four genomic regions were associated with resistance in the DH and F 2:3 populations, respectively. They collectively explained 36 to 42% of the variation within each year and population. Three of them were consistent across the two populations derived from Darmor × Samourai cross and expressed dominant or overdominant effects. Four favorable alleles were derived from the susceptible parent. A total of 16 genomic regions were revealed for blackleg resistance in the two crosses Darmor‐ bzh × Yudal and Darmor × Samourai studied. Four of them were consistent over the two crosses. The inconsistencies observed between populations and crosses can be explained by different genetic backgrounds and disease infestation levels. For marker‐assisted selection, these results suggest that QTL mapping must be carried out separately for each population." @default.
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- W2037535826 date "2001-01-01" @default.
- W2037535826 modified "2023-10-14" @default.
- W2037535826 title "Stability of QTL for Field Resistance to Blackleg across Two Genetic Backgrounds in Oilseed Rape" @default.
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- W2037535826 doi "https://doi.org/10.2135/cropsci2001.411197x" @default.
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