FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2037540104> ?p ?o ?g. }

• W2037540104 endingPage "4083" @default.
• W2037540104 startingPage "4083" @default.
• W2037540104 abstract "Over the past few decades, Fourier transform infrared (FTIR) spectroscopy coupled to microscopy has been recognized as an emerging and potentially powerful tool in cancer research and diagnosis. For this purpose, histological analyses performed by pathologists are mostly carried out on biopsied tissue that undergoes the formalin-fixation and paraffin-embedding (FFPE) procedure. This processing method ensures an optimal and permanent preservation of the samples, making FFPE-archived tissue an extremely valuable source for retrospective studies. Nevertheless, as highlighted by previous studies, this fixation procedure significantly changes the principal constituents of cells, resulting in important effects on their infrared (IR) spectrum. Despite the chemical and spectral influence of FFPE processing, some studies demonstrate that FTIR imaging allows precise identification of the different cell types present in biopsied tissue, indicating that the FFPE process preserves spectral differences between distinct cell types. In this study, we investigated whether this is also the case for closely related cell lines. We analyzed spectra from 8 cancerous epithelial cell lines: 4 breast cancer cell lines and 4 melanoma cell lines. For each cell line, we harvested cells at subconfluence and divided them into two sets. We first tested the “original” capability of FTIR imaging to identify these closely related cell lines on cells just dried on BaF2 slides. We then repeated the test after submitting the cells to the FFPE procedure. Our results show that the IR spectra of FFPE processed cancerous cell lines undergo small but significant changes due to the treatment. The spectral modifications were interpreted as a potential decrease in the phospholipid content and protein denaturation, in line with the scientific literature on the topic. Nevertheless, unsupervised analyses showed that spectral proximities and distances between closely related cell lines were mostly, but not entirely, conserved after FFPE processing. Finally, PLS-DA statistical analyses highlighted that closely related cell lines are still successfully identified and efficiently distinguished by FTIR spectroscopy after FFPE treatment. This last result paves the way towards identification and characterization of cellular subtypes on FFPE tissue sections by FTIR imaging, indicating that this analysis technique could become a potential useful tool in cancer research." @default.
• W2037540104 created "2016-06-24" @default.
• W2037540104 creator A5000647003 @default.
• W2037540104 creator A5003037297 @default.
• W2037540104 creator A5011197999 @default.
• W2037540104 creator A5023233738 @default.
• W2037540104 creator A5025224681 @default.
• W2037540104 creator A5029687188 @default.
• W2037540104 creator A5034256205 @default.
• W2037540104 creator A5049771372 @default.
• W2037540104 creator A5057269336 @default.
• W2037540104 creator A5079146691 @default.
• W2037540104 creator A5088407379 @default.
• W2037540104 date "2013-01-01" @default.
• W2037540104 modified "2023-10-16" @default.
• W2037540104 title "Breast cancer and melanoma cell line identification by FTIR imaging after formalin-fixation and paraffin-embedding" @default.
• W2037540104 cites W1683474607 @default.
• W2037540104 cites W1967139820 @default.
• W2037540104 cites W1974000725 @default.
• W2037540104 cites W1981753113 @default.
• W2037540104 cites W1985617195 @default.
• W2037540104 cites W1986846505 @default.
• W2037540104 cites W1987389391 @default.
• W2037540104 cites W1987690242 @default.
• W2037540104 cites W1990005198 @default.
• W2037540104 cites W1993186794 @default.
• W2037540104 cites W1996243268 @default.
• W2037540104 cites W2003092713 @default.
• W2037540104 cites W2015151924 @default.
• W2037540104 cites W2016381774 @default.
• W2037540104 cites W2018832912 @default.
• W2037540104 cites W2019564564 @default.
• W2037540104 cites W2024971501 @default.
• W2037540104 cites W2026140194 @default.
• W2037540104 cites W2036936602 @default.
• W2037540104 cites W2038334009 @default.
• W2037540104 cites W2042754732 @default.
• W2037540104 cites W2043197681 @default.
• W2037540104 cites W2064359917 @default.
• W2037540104 cites W2066943809 @default.
• W2037540104 cites W2067822080 @default.
• W2037540104 cites W2083522386 @default.
• W2037540104 cites W2090992793 @default.
• W2037540104 cites W2091797019 @default.
• W2037540104 cites W2097581544 @default.
• W2037540104 cites W2100936474 @default.
• W2037540104 cites W2113001325 @default.
• W2037540104 cites W2121134512 @default.
• W2037540104 cites W2126521392 @default.
• W2037540104 cites W2134272188 @default.
• W2037540104 cites W2141609711 @default.
• W2037540104 cites W2159920173 @default.
• W2037540104 cites W2160797083 @default.
• W2037540104 cites W2170034127 @default.
• W2037540104 cites W4230964105 @default.
• W2037540104 cites W54859794 @default.
• W2037540104 doi "https://doi.org/10.1039/c3an00246b" @default.
• W2037540104 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23689823" @default.
• W2037540104 hasPublicationYear "2013" @default.
• W2037540104 type Work @default.
• W2037540104 sameAs 2037540104 @default.
• W2037540104 citedByCount "42" @default.
• W2037540104 countsByYear W20375401042014 @default.
• W2037540104 countsByYear W20375401042015 @default.
• W2037540104 countsByYear W20375401042016 @default.
• W2037540104 countsByYear W20375401042017 @default.
• W2037540104 countsByYear W20375401042018 @default.
• W2037540104 countsByYear W20375401042019 @default.
• W2037540104 countsByYear W20375401042020 @default.
• W2037540104 countsByYear W20375401042021 @default.
• W2037540104 countsByYear W20375401042022 @default.
• W2037540104 countsByYear W20375401042023 @default.
• W2037540104 crossrefType "journal-article" @default.
• W2037540104 hasAuthorship W2037540104A5000647003 @default.
• W2037540104 hasAuthorship W2037540104A5003037297 @default.
• W2037540104 hasAuthorship W2037540104A5011197999 @default.
• W2037540104 hasAuthorship W2037540104A5023233738 @default.
• W2037540104 hasAuthorship W2037540104A5025224681 @default.
• W2037540104 hasAuthorship W2037540104A5029687188 @default.
• W2037540104 hasAuthorship W2037540104A5034256205 @default.
• W2037540104 hasAuthorship W2037540104A5049771372 @default.
• W2037540104 hasAuthorship W2037540104A5057269336 @default.
• W2037540104 hasAuthorship W2037540104A5079146691 @default.
• W2037540104 hasAuthorship W2037540104A5088407379 @default.
• W2037540104 hasConcept C104317684 @default.
• W2037540104 hasConcept C120665830 @default.
• W2037540104 hasConcept C121332964 @default.
• W2037540104 hasConcept C136229726 @default.
• W2037540104 hasConcept C142724271 @default.
• W2037540104 hasConcept C146249460 @default.
• W2037540104 hasConcept C1491633281 @default.
• W2037540104 hasConcept C160892712 @default.
• W2037540104 hasConcept C185592680 @default.
• W2037540104 hasConcept C2777658100 @default.
• W2037540104 hasConcept C502942594 @default.
• W2037540104 hasConcept C54355233 @default.
• W2037540104 hasConcept C55493867 @default.
• W2037540104 hasConcept C71924100 @default.

• next