FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2037547488> ?p ?o ?g. }

• W2037547488 endingPage "1527" @default.
• W2037547488 startingPage "1519" @default.
• W2037547488 abstract "Eight variants of creatine kinase were created to switch the substrate specificity from creatine to glycocyamine using a rational design approach. Changes to creatine kinase involved altering several residues on the flexible loops that fold over the bound substrates including a chimeric replacement of the guanidino specificity loop from glycocyamine kinase into creatine kinase. A maximal 2000-fold change in substrate specificity was obtained as measured by a ratio of enzymatic efficiency (kcat/KM · Kd) for creatine vs. glycocyamine. In all cases, a change in specificity was accompanied by a large drop in enzymatic efficiency. This data, combined with evidence from other studies, indicate that substrate specificity in the phosphagen kinase family is obtained by precise alignment of substrates in the active site to maximize kcat/KM · Kd as opposed to selective molecular recognition of one guanidino substrate over another. A model for the evolution of the dimeric forms of phosphagen kinases is proposed in which these enzymes radiated from a common ancestor that may have possessed a level of catalytic promiscuity. As mutational events occurred leading to greater degrees of substrate specificity, the dimeric phosphagen kinases became evolutionary separated such that the substrate specificity could not be interchanged by a small number of mutations." @default.
• W2037547488 created "2016-06-24" @default.
• W2037547488 creator A5000679063 @default.
• W2037547488 creator A5004968327 @default.
• W2037547488 creator A5015381344 @default.
• W2037547488 creator A5041091292 @default.
• W2037547488 creator A5047935420 @default.
• W2037547488 creator A5048023467 @default.
• W2037547488 creator A5058585973 @default.
• W2037547488 creator A5066402048 @default.
• W2037547488 creator A5082975504 @default.
• W2037547488 date "2007-12-01" @default.
• W2037547488 modified "2023-09-25" @default.
• W2037547488 title "Changing the substrate specificity of creatine kinase from creatine to glycocyamine: Evidence for a highly evolved active site" @default.
• W2037547488 cites W145825086 @default.
• W2037547488 cites W1571498771 @default.
• W2037547488 cites W1638372631 @default.
• W2037547488 cites W1849159432 @default.
• W2037547488 cites W1916101590 @default.
• W2037547488 cites W1965354567 @default.
• W2037547488 cites W1969645229 @default.
• W2037547488 cites W1971428288 @default.
• W2037547488 cites W1975210435 @default.
• W2037547488 cites W1983245341 @default.
• W2037547488 cites W1986005076 @default.
• W2037547488 cites W1995344463 @default.
• W2037547488 cites W1995937823 @default.
• W2037547488 cites W1997028350 @default.
• W2037547488 cites W1998238795 @default.
• W2037547488 cites W2000879513 @default.
• W2037547488 cites W2001241436 @default.
• W2037547488 cites W2010918777 @default.
• W2037547488 cites W2015380104 @default.
• W2037547488 cites W2016123989 @default.
• W2037547488 cites W2023922395 @default.
• W2037547488 cites W2045309480 @default.
• W2037547488 cites W2053329213 @default.
• W2037547488 cites W2055772661 @default.
• W2037547488 cites W2056214680 @default.
• W2037547488 cites W2060854559 @default.
• W2037547488 cites W2070796678 @default.
• W2037547488 cites W2079635362 @default.
• W2037547488 cites W2081158065 @default.
• W2037547488 cites W2091358596 @default.
• W2037547488 cites W2094326993 @default.
• W2037547488 cites W2101280466 @default.
• W2037547488 cites W2111428654 @default.
• W2037547488 cites W2124241920 @default.
• W2037547488 cites W2132629607 @default.
• W2037547488 cites W2142195662 @default.
• W2037547488 cites W2144699036 @default.
• W2037547488 cites W2170996473 @default.
• W2037547488 cites W2178313645 @default.
• W2037547488 cites W244589956 @default.
• W2037547488 cites W2969273703 @default.
• W2037547488 cites W326933087 @default.
• W2037547488 cites W4241414174 @default.
• W2037547488 cites W4254999352 @default.
• W2037547488 cites W61548541 @default.
• W2037547488 cites W80995729 @default.
• W2037547488 cites W2018949027 @default.
• W2037547488 doi "https://doi.org/10.1016/j.bbapap.2007.10.001" @default.
• W2037547488 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17976392" @default.
• W2037547488 hasPublicationYear "2007" @default.
• W2037547488 type Work @default.
• W2037547488 sameAs 2037547488 @default.
• W2037547488 citedByCount "12" @default.
• W2037547488 countsByYear W20375474882013 @default.
• W2037547488 countsByYear W20375474882015 @default.
• W2037547488 countsByYear W20375474882019 @default.
• W2037547488 countsByYear W20375474882020 @default.
• W2037547488 crossrefType "journal-article" @default.
• W2037547488 hasAuthorship W2037547488A5000679063 @default.
• W2037547488 hasAuthorship W2037547488A5004968327 @default.
• W2037547488 hasAuthorship W2037547488A5015381344 @default.
• W2037547488 hasAuthorship W2037547488A5041091292 @default.
• W2037547488 hasAuthorship W2037547488A5047935420 @default.
• W2037547488 hasAuthorship W2037547488A5048023467 @default.
• W2037547488 hasAuthorship W2037547488A5058585973 @default.
• W2037547488 hasAuthorship W2037547488A5066402048 @default.
• W2037547488 hasAuthorship W2037547488A5082975504 @default.
• W2037547488 hasConcept C181199279 @default.
• W2037547488 hasConcept C184235292 @default.
• W2037547488 hasConcept C185592680 @default.
• W2037547488 hasConcept C18903297 @default.
• W2037547488 hasConcept C2777289219 @default.
• W2037547488 hasConcept C2779896295 @default.
• W2037547488 hasConcept C36880943 @default.
• W2037547488 hasConcept C41183919 @default.
• W2037547488 hasConcept C55493867 @default.
• W2037547488 hasConcept C56856141 @default.
• W2037547488 hasConcept C71240020 @default.
• W2037547488 hasConcept C86803240 @default.
• W2037547488 hasConceptScore W2037547488C181199279 @default.
• W2037547488 hasConceptScore W2037547488C184235292 @default.
• W2037547488 hasConceptScore W2037547488C185592680 @default.
• W2037547488 hasConceptScore W2037547488C18903297 @default.
• W2037547488 hasConceptScore W2037547488C2777289219 @default.

• next