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• W2037561935 abstract "A capillary electrophoresis-inductively coupled plasma mass spectrometric (CE-ICP-MS) method for the determination of cobalamin is described. Samples of cobalt-containing compounds were subjected to electrophoretic separation before injection into the microconcentric nebulizer (CEI-100) of ICP-MS. The Co-containing compounds studied include cyanocobalamin (CN-Cbl, vitamin B12), hydroxocobalamin (OH-Cbl), and Co(II). The species studied were well separated using a 70 cm lengthx75 microm i.d. fused silica capillary with the applied voltage set at +20 kV. A 25 mM Tris buffer (pH 9.0) containing 15 mM sodium dodecyl sulfate (SDS) was used as the electrophoretic buffer. The CE-ICP-MS detection limits were 0.3, 0.2, and 1.7 ng of Co mL(-1) for CN-Cbl, OH-Cbl, and Co(II), respectively. The concentrations of cobalamin compounds were determined in selected nutritive supplements and chlorella samples. A microwave-assisted extraction method was used for the extraction of these compounds. Over 92% of the total cobalt species was extracted using a 5% v/v HNO3 solution in a focused microwave field within a period of 10 min; spike recovery was in the range of 94-105% for various species. The major cobalt species in nutritive supplements and chlorella samples was cyanocobalamin." @default.
• W2037561935 created "2016-06-24" @default.
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• W2037561935 date "2008-02-01" @default.
• W2037561935 modified "2023-09-24" @default.
• W2037561935 title "Determination of Cobalamin in Nutritive Supplements and Chlorella Foods by Capillary Electrophoresis−Inductively Coupled Plasma Mass Spectrometry" @default.
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• W2037561935 doi "https://doi.org/10.1021/jf073213h" @default.
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