FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2037562977> ?p ?o ?g. }

• W2037562977 endingPage "e6606" @default.
• W2037562977 startingPage "e6606" @default.
• W2037562977 abstract "Background Stromal-Derived Inducing Activity (SDIA) is one of the most efficient methods of generating dopaminergic (DA) neurons from embryonic stem cells (ESC). DA neuron induction can be achieved by co-culturing ESC with the mouse stromal cell lines PA6 or MS5. The molecular nature of this effect, which has been termed “SDIA” is so far unknown. Recently, we found that factors secreted by PA6 cells provided lineage-specific instructions to induce DA differentiation of human ESC (hESC). Methodology/Principal Findings In the present study, we compared PA6 cells to various cell lines lacking the SDIA effect, and employed genome expression analysis to identify differentially-expressed signaling molecules. Among the factors highly expressed by PA6 cells, and known to be associated with CNS development, were stromal cell-derived factor 1 (SDF-1/CXCL12), pleiotrophin (PTN), insulin-like growth factor 2 (IGF2), and ephrin B1 (EFNB1). When these four factors, the combination of which was termed SPIE, were applied to hESC, they induced differentiation to TH-positive neurons in vitro. RT-PCR and western blot analysis confirmed the expression of midbrain specific markers, including engrailed 1, Nurr1, Pitx3, and dopamine transporter (DAT) in cultures influenced by these four molecules. Electrophysiological recordings showed that treatment of hESC with SPIE induced differentiation of neurons that were capable of generating action potentials and forming functional synaptic connections. Conclusions/Significance The combination of SDF-1, PTN, IGF2, and EFNB1 mimics the DA phenotype-inducing property of SDIA and was sufficient to promote differentiation of hESC to functional midbrain DA neurons. These findings provide a method for differentiating hESC to form DA neurons, without a requirement for the use of animal-derived cell lines or products." @default.
• W2037562977 created "2016-06-24" @default.
• W2037562977 creator A5021804109 @default.
• W2037562977 creator A5024007741 @default.
• W2037562977 creator A5026648631 @default.
• W2037562977 creator A5028400865 @default.
• W2037562977 creator A5030149513 @default.
• W2037562977 creator A5049419210 @default.
• W2037562977 creator A5052081713 @default.
• W2037562977 creator A5069694896 @default.
• W2037562977 date "2009-08-12" @default.
• W2037562977 modified "2023-10-16" @default.
• W2037562977 title "A Novel Combination of Factors, Termed SPIE, which Promotes Dopaminergic Neuron Differentiation from Human Embryonic Stem Cells" @default.
• W2037562977 cites W1507664203 @default.
• W2037562977 cites W1526738019 @default.
• W2037562977 cites W1601656880 @default.
• W2037562977 cites W1606843899 @default.
• W2037562977 cites W1622452015 @default.
• W2037562977 cites W1769886694 @default.
• W2037562977 cites W1959046600 @default.
• W2037562977 cites W1966476278 @default.
• W2037562977 cites W1968009280 @default.
• W2037562977 cites W1974521830 @default.
• W2037562977 cites W1982501879 @default.
• W2037562977 cites W1983274440 @default.
• W2037562977 cites W1988791308 @default.
• W2037562977 cites W1989915490 @default.
• W2037562977 cites W1990057474 @default.
• W2037562977 cites W1996449721 @default.
• W2037562977 cites W1996492268 @default.
• W2037562977 cites W2002930046 @default.
• W2037562977 cites W2004338203 @default.
• W2037562977 cites W2005296007 @default.
• W2037562977 cites W2014572511 @default.
• W2037562977 cites W2020005796 @default.
• W2037562977 cites W2021721582 @default.
• W2037562977 cites W2029115771 @default.
• W2037562977 cites W2030997373 @default.
• W2037562977 cites W2033290532 @default.
• W2037562977 cites W2034302726 @default.
• W2037562977 cites W2036094320 @default.
• W2037562977 cites W2036834043 @default.
• W2037562977 cites W2043300325 @default.
• W2037562977 cites W2043713881 @default.
• W2037562977 cites W2054810445 @default.
• W2037562977 cites W2062849851 @default.
• W2037562977 cites W2066077301 @default.
• W2037562977 cites W2066790769 @default.
• W2037562977 cites W2068602040 @default.
• W2037562977 cites W2072158957 @default.
• W2037562977 cites W2078385953 @default.
• W2037562977 cites W2083939751 @default.
• W2037562977 cites W2084412384 @default.
• W2037562977 cites W2084565997 @default.
• W2037562977 cites W2089734647 @default.
• W2037562977 cites W2090217399 @default.
• W2037562977 cites W2098509726 @default.
• W2037562977 cites W2099002558 @default.
• W2037562977 cites W2102091298 @default.
• W2037562977 cites W2107983150 @default.
• W2037562977 cites W2109972689 @default.
• W2037562977 cites W2110940238 @default.
• W2037562977 cites W2111191613 @default.
• W2037562977 cites W2114737664 @default.
• W2037562977 cites W2116715929 @default.
• W2037562977 cites W2124388496 @default.
• W2037562977 cites W2128087515 @default.
• W2037562977 cites W2140598166 @default.
• W2037562977 cites W2147015050 @default.
• W2037562977 cites W2150094887 @default.
• W2037562977 cites W2151076343 @default.
• W2037562977 cites W2161276023 @default.
• W2037562977 cites W2162811821 @default.
• W2037562977 cites W2171248814 @default.
• W2037562977 cites W2171610429 @default.
• W2037562977 cites W2172279192 @default.
• W2037562977 cites W2264038960 @default.
• W2037562977 cites W2418088621 @default.
• W2037562977 doi "https://doi.org/10.1371/journal.pone.0006606" @default.
• W2037562977 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2719871" @default.
• W2037562977 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19672298" @default.
• W2037562977 hasPublicationYear "2009" @default.
• W2037562977 type Work @default.
• W2037562977 sameAs 2037562977 @default.
• W2037562977 citedByCount "83" @default.
• W2037562977 countsByYear W20375629772012 @default.
• W2037562977 countsByYear W20375629772013 @default.
• W2037562977 countsByYear W20375629772014 @default.
• W2037562977 countsByYear W20375629772015 @default.
• W2037562977 countsByYear W20375629772016 @default.
• W2037562977 countsByYear W20375629772017 @default.
• W2037562977 countsByYear W20375629772018 @default.
• W2037562977 countsByYear W20375629772019 @default.
• W2037562977 countsByYear W20375629772020 @default.
• W2037562977 countsByYear W20375629772021 @default.
• W2037562977 countsByYear W20375629772022 @default.
• W2037562977 countsByYear W20375629772023 @default.
• W2037562977 crossrefType "journal-article" @default.

• next