FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2037620194> ?p ?o ?g. }

• W2037620194 endingPage "357" @default.
• W2037620194 startingPage "350" @default.
• W2037620194 abstract "A number of flow cytometric assays for natural killer (NK) cell cytotoxicity have been described, however, the relative merits of analytical approaches and the influence of platelets on measured responses have not been systematically evaluated. Information on the time-dependent variability in measured responses is also limited.Human peripheral blood mononuclear cells were obtained using Nycoprep 1.077, or Nycoprep 1.077 followed by Nycoprep 1.068 (to remove platelets), and incubated for 3 hours with MitoTracker Green (MTG)-labeled K562 cells. Cells were stained with propidium iodide (PI) and the proportions of viable and nonviable target cells (MTG(+)PI(-), MTG(+)PI(+)) were determined by flow cytometry using quadrant and polygonal region analysis.Platelets inhibited NK cell cytotoxicity and the response was underestimated when the nonviable target cell population was not entirely enclosed within the nonviable target cell (upper right) flow cytometric quadrant. The variability in measured NK cell cytotoxic responses in samples obtained from five individuals on three occasions over a 3-week period was 28%, 24%, 26%, and 37%, and 19%, 23%, 27%, and 32% for the quadrant and region analyses (mean coefficient of variation at effector-to-target cell ratios of 100:1, 50:1, 25:1, and 12.5:1, respectively), and 24% and 20% when data were calculated as the area under the cytotoxic curve (AUCC).Polygonal regions and the calculation of data as the AUCC appear to be the best approach. This study will be of value to investigators that are wishing to incorporate an NK cell cytotoxicity assay into their portfolio of experimental techniques." @default.
• W2037620194 created "2016-06-24" @default.
• W2037620194 creator A5000919554 @default.
• W2037620194 creator A5010503374 @default.
• W2037620194 creator A5057129908 @default.
• W2037620194 creator A5069510718 @default.
• W2037620194 creator A5073973049 @default.
• W2037620194 creator A5074201843 @default.
• W2037620194 creator A5081754463 @default.
• W2037620194 date "2007-03-01" @default.
• W2037620194 modified "2023-09-25" @default.
• W2037620194 title "A MitoTracker Green-based flow cytometric assay for natural killer cell activity: Variability, the influence of platelets and a comparison of analytical approaches" @default.
• W2037620194 cites W1489638429 @default.
• W2037620194 cites W1594577646 @default.
• W2037620194 cites W1602367925 @default.
• W2037620194 cites W1986170848 @default.
• W2037620194 cites W1994538641 @default.
• W2037620194 cites W1999880220 @default.
• W2037620194 cites W2011036671 @default.
• W2037620194 cites W2011647890 @default.
• W2037620194 cites W2013148395 @default.
• W2037620194 cites W2037883923 @default.
• W2037620194 cites W2042356157 @default.
• W2037620194 cites W2051051921 @default.
• W2037620194 cites W2052232839 @default.
• W2037620194 cites W2059995303 @default.
• W2037620194 cites W2067482760 @default.
• W2037620194 cites W2072159436 @default.
• W2037620194 cites W2100482220 @default.
• W2037620194 cites W2128437356 @default.
• W2037620194 cites W2149605983 @default.
• W2037620194 cites W6875991 @default.
• W2037620194 doi "https://doi.org/10.1016/j.exphem.2006.12.001" @default.
• W2037620194 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17309815" @default.
• W2037620194 hasPublicationYear "2007" @default.
• W2037620194 type Work @default.
• W2037620194 sameAs 2037620194 @default.
• W2037620194 citedByCount "12" @default.
• W2037620194 countsByYear W20376201942012 @default.
• W2037620194 countsByYear W20376201942013 @default.
• W2037620194 countsByYear W20376201942016 @default.
• W2037620194 countsByYear W20376201942017 @default.
• W2037620194 countsByYear W20376201942020 @default.
• W2037620194 crossrefType "journal-article" @default.
• W2037620194 hasAuthorship W2037620194A5000919554 @default.
• W2037620194 hasAuthorship W2037620194A5010503374 @default.
• W2037620194 hasAuthorship W2037620194A5057129908 @default.
• W2037620194 hasAuthorship W2037620194A5069510718 @default.
• W2037620194 hasAuthorship W2037620194A5073973049 @default.
• W2037620194 hasAuthorship W2037620194A5074201843 @default.
• W2037620194 hasAuthorship W2037620194A5081754463 @default.
• W2037620194 hasBestOaLocation W20376201941 @default.
• W2037620194 hasConcept C109316439 @default.
• W2037620194 hasConcept C137061746 @default.
• W2037620194 hasConcept C1491633281 @default.
• W2037620194 hasConcept C153911025 @default.
• W2037620194 hasConcept C154317977 @default.
• W2037620194 hasConcept C185592680 @default.
• W2037620194 hasConcept C190283241 @default.
• W2037620194 hasConcept C202751555 @default.
• W2037620194 hasConcept C203014093 @default.
• W2037620194 hasConcept C2775934118 @default.
• W2037620194 hasConcept C2778102761 @default.
• W2037620194 hasConcept C2908647359 @default.
• W2037620194 hasConcept C31573885 @default.
• W2037620194 hasConcept C553184892 @default.
• W2037620194 hasConcept C55493867 @default.
• W2037620194 hasConcept C71924100 @default.
• W2037620194 hasConcept C86803240 @default.
• W2037620194 hasConcept C89560881 @default.
• W2037620194 hasConcept C99454951 @default.
• W2037620194 hasConceptScore W2037620194C109316439 @default.
• W2037620194 hasConceptScore W2037620194C137061746 @default.
• W2037620194 hasConceptScore W2037620194C1491633281 @default.
• W2037620194 hasConceptScore W2037620194C153911025 @default.
• W2037620194 hasConceptScore W2037620194C154317977 @default.
• W2037620194 hasConceptScore W2037620194C185592680 @default.
• W2037620194 hasConceptScore W2037620194C190283241 @default.
• W2037620194 hasConceptScore W2037620194C202751555 @default.
• W2037620194 hasConceptScore W2037620194C203014093 @default.
• W2037620194 hasConceptScore W2037620194C2775934118 @default.
• W2037620194 hasConceptScore W2037620194C2778102761 @default.
• W2037620194 hasConceptScore W2037620194C2908647359 @default.
• W2037620194 hasConceptScore W2037620194C31573885 @default.
• W2037620194 hasConceptScore W2037620194C553184892 @default.
• W2037620194 hasConceptScore W2037620194C55493867 @default.
• W2037620194 hasConceptScore W2037620194C71924100 @default.
• W2037620194 hasConceptScore W2037620194C86803240 @default.
• W2037620194 hasConceptScore W2037620194C89560881 @default.
• W2037620194 hasConceptScore W2037620194C99454951 @default.
• W2037620194 hasIssue "3" @default.
• W2037620194 hasLocation W20376201941 @default.
• W2037620194 hasLocation W20376201942 @default.
• W2037620194 hasOpenAccess W2037620194 @default.
• W2037620194 hasPrimaryLocation W20376201941 @default.
• W2037620194 hasRelatedWork W1998387701 @default.
• W2037620194 hasRelatedWork W2028761577 @default.
• W2037620194 hasRelatedWork W2037883923 @default.

• next