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- W2037637586 abstract "Conservation plans for freshwater mussels include enhancing natural populations with artificially cultured individuals. Chemical marks on cultured juveniles would provide a way to evaluate the success of those efforts. We immersed Lampsilis cardium and Actinonaias pectorosa juveniles of various ages (1-d–210-d-old) in either oxytetracycline (OTC) or calcein (125–500 mg/L) for different lengths of time (3–48 h) and assessed growth, survival, and mark retention. There were no immediate lethal effects on 1-d-old L. cardium after a 24-h immersion in either chemical at a concentration of 500 mg/L. A single immersion in 250 mg/L OTC of 1-mo-old L. cardium inhibited growth for 2 mo post-immersion, and multiple immersions also affected survival. Neither growth nor survival were affected when older mussels (≥2-mo old) were immersed. Fluorescent marks were laid down primarily in the growing edge of the shell as a ring extending around the mussel, and marking success increased with concentration and immersion time. Immersing 7-mo-old L. cardium in 250 mg/L OTC for 24 h produced marks visible in all thin-sections examined 6 mo later; however, marks were not visible in whole shells. In contrast, distinctive calcein marks were visible not only in thin-sections but also on all whole 7-mo-old L. cardium shells 6 mo after mussels were immersed for 24 h at a concentration of 250 mg/L. A 12-h immersion at the same concentration produced inconsistent calcein marks in 5-mo-old L. cardium but yielded >90% correct mark identification in live 7-mo-old A. pectorosa 1 y after immersion. The results of our study indicate that persistent and readily identifiable chemical marks can be produced in juvenile mussels of some species ≥2-mo-old by a 24-h immersion in 250 mg/L calcein or OTC with little to no adverse effects on the mussels." @default.
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- W2037637586 date "2002-09-01" @default.
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- W2037637586 title "How to pick your mussels out of a crowd: using fluorescence to mark juvenile freshwater mussels" @default.
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- W2037637586 doi "https://doi.org/10.2307/1468484" @default.
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