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- W2037653553 abstract "The cytokine inducing capacity of the vaccine vector pcDNA3, a methylated form of the plasmid, and pcDNA3 encoding porcine interleukin (IL)-6 or granulocyte/macrophage colony-stimulating factor (GM-CSF) was studied in pigs, using a model with tissue chambers implanted subcutaneously. The production of interferon (IFN)-alpha, IFN-gamma, IL-6 and GM-CSF was studied at local (tissue chamber fluid (TCF)) and systemic (serum) levels during 3 days post-injection. All forms of the plasmid, except the methylated, induced a transient local production of IFN-alpha but no plasmid-induced production of IFN-gamma, GM-CSF or IL-6 could be detected after injection of the plasmids. The IFN-alpha response increased markedly at repeated injections of pcDNA3. This IFN-alpha inducing capacity of the plasmid is likely to affect immune responses at DNA vaccination of pigs." @default.
- W2037653553 created "2016-06-24" @default.
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- W2037653553 date "2002-08-01" @default.
- W2037653553 modified "2023-10-11" @default.
- W2037653553 title "The DNA vaccine vector pcDNA3 induces IFN-α production in pigs" @default.
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- W2037653553 doi "https://doi.org/10.1016/s0165-2427(02)00024-7" @default.
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