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• W2037677961 startingPage "498" @default.
• W2037677961 abstract "Attachment of virulent Agrobacterium tumefaciens to plant cells is required for transformation. To further study the components of the plant cell wall that may be involved in the attachment process, tobacco (Nicotiana tabacum L.) protoplasts were cultured in the presence of 2,6 dichlorobenzonitrile (DB), an inhibitor of cellulose biosynthesis, and then assayed for their ability to be transformed by Agrobacterium. The DB treated protoplasts were deficient in wall production. Nevertheless, they were transformable at high frequency by wild type Agrobacterium strains but not by mutant strains that lack the ability to bind to normal, walled cells. Small quantities of calcofluor white positive material present on DB treated cells were correlated with their competence to be transformed. Further, the plant:bacterial association that leads to transformation is shown to become stable within 5 hours after bacterial co-cultivation with either control or DB treated cells." @default.
• W2037677961 created "2016-06-24" @default.
• W2037677961 creator A5041207516 @default.
• W2037677961 date "1991-06-01" @default.
• W2037677961 modified "2023-10-18" @default.
• W2037677961 title "Transformation of Wall Deficient Cultured Tobacco Protoplasts by <i>Agrobacterium tumefaciens</i>" @default.
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• W2037677961 doi "https://doi.org/10.1104/pp.96.2.498" @default.
• W2037677961 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1080798" @default.
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