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• W2037752308 endingPage "107" @default.
• W2037752308 startingPage "103" @default.
• W2037752308 abstract "A novel labeling-free fluorescence complex probe has been developed for DNA hybridization detection based on fluorescence resonance energy transfer (FRET) mechanism from pyrene excimer of pyrene-functionalized poly [2-(N, N-dimethylamino) ethyl methacrylate] (PFP) to SYBR Green I (SG, a specific intercalator of double-stranded DNA) in a cost-effective, rapid and simple manner. The complex probe consists of the positively charged PFP, SG and negatively charged single-stranded DNA (ssDNA). Upon adding a complementary strand to the complex probe solution, double-stranded DNA (dsDNA) was formed, followed by the intercalation of SG into dsDNA. The pyrene excimer emission was overlapped with the absorption of SG very well and the electrostatic interactions between PFP and dsDNA kept them in close proximity, enabling efficient FRET from pyrene excimer to SG. The fluorescence of SG in the duplex DNA resulting from FRET can be successfully applied to detect DNA hybridization with high sensitivity for a very low detection limit of 10nM and excellent selectivity for detection of single base pair mismatch." @default.
• W2037752308 created "2016-06-24" @default.
• W2037752308 creator A5004795100 @default.
• W2037752308 creator A5046418078 @default.
• W2037752308 creator A5076155990 @default.
• W2037752308 creator A5079633091 @default.
• W2037752308 date "2015-03-01" @default.
• W2037752308 modified "2023-10-17" @default.
• W2037752308 title "Labeling-free fluorescent detection of DNA hybridization through FRET from pyrene excimer to DNA intercalator SYBR green I" @default.
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• W2037752308 doi "https://doi.org/10.1016/j.bios.2014.10.029" @default.
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• W2037752308 hasPublicationYear "2015" @default.
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