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• W2037757717 startingPage "702" @default.
• W2037757717 abstract "We report a capillary-based DNA sequencing read length of 100 bases in 16 min using end-labeled free-solution conjugate electrophoresis (FSCE) with a monodisperse poly-N-substituted glycine (polypeptoid) as a synthetic drag-tag. FSCE enabled rapid separation of single-stranded (ss) DNA sequencing fragments with single-base resolution without the need for a viscous DNA separation matrix. Protein-based drag-tags previously used for FSCE sequencing, for example, streptavidin, are heterogeneous in molar mass (polydisperse); the resultant band-broadening can make it difficult to obtain the single-base resolution necessary for DNA sequencing. In this study, we synthesized and HPLC-purified a 70mer poly-N-(methoxyethyl)glycine (NMEG) drag-tag with a molar mass of - 11 kDa. The NMEG monomers that comprise this peptoid drag-tag are interesting for bioanalytical applications, because the methoxyethyl side chain's chemical structure is reminiscent of the basic monomer unit of polyethylene glycol, a highly biocompatible commercially available polymer, which, however, is not available in monodisperse preparation at an - 11 kDa molar mass. This is the first report of ssDNA separation and of four-color, base-by-base DNA sequencing by FSCE through the use of a chemically synthesized drag-tag. These results show that high-molar mass, chemically synthesized drag-tags based on the polyNMEG structure, if obtained in monodisperse preparation, would serve as ideal drag-tags and could help FSCE reach the commercially relevant read lengths of 100 bases or more." @default.
• W2037757717 created "2016-06-24" @default.
• W2037757717 creator A5013082584 @default.
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• W2037757717 date "2011-01-01" @default.
• W2037757717 modified "2023-09-25" @default.
• W2037757717 title "A chemically synthesized peptoid-based drag-tag enhances free-solution DNA sequencing by capillary electrophoresis" @default.
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• W2037757717 doi "https://doi.org/10.1002/bip.21627" @default.
• W2037757717 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3425634" @default.
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