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- W2037791644 abstract "Abstract Natural 15 N abundances (δ 15 N values) of different soil nitrogen pools deliver crucial information on the soil N cycle for the analysis of biogeochemical processes. Here we report on a complete suite of methods for sensitive δ 15 N analysis in soil extracts. A combined chemical reaction of vanadium(III) chloride (VCl 3 ) and sodium azide under acidic conditions is used to convert nitrate into N 2 O, which is subsequently analyzed by purge‐and‐trap isotope ratio mass spectrometry (PTIRMS) with a cryo‐focusing unit. Coupled with preparation steps (microdiffusion for collection of ammonium, alkaline persulfate oxidation to convert total dissolved N (TDN) or ammonium into nitrate) this allows the determination of the δ 15 N values of nitrate, ammonium and total dissolved N (dissolved organic N, microbial biomass N) in soil extracts with the same basic protocol. The limits of quantification for δ 15 N analysis with a precision of 0.5‰ were 12.4 µM for ammonium, 23.7 µM for TDN, 16.5 µM for nitrate and 22.7 µM for nitrite. Copyright © 2010 John Wiley & Sons, Ltd." @default.
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- W2037791644 date "2010-11-15" @default.
- W2037791644 modified "2023-10-14" @default.
- W2037791644 title "A suite of sensitive chemical methods to determine the δ<sup>15</sup>N of ammonium, nitrate and total dissolved N in soil extracts" @default.
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- W2037791644 doi "https://doi.org/10.1002/rcm.4798" @default.
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