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- W2037834206 abstract "Strawberry fruit shows a marked softening during ripening and the process is associated with an increment of pectin solubility and a reduction of the molecular mass of hemicelluloses. In this work, we report the activity of β-xylosidase and the expression of a β-xylosidase gene in strawberry fruit. We have cloned a cDNA fragment encoding a putative β-xylosidase (FaXyl1) from a cDNA library obtained from ripe strawberry fruit. The analysis of the deduced amino acid sequence revealed that FaXyl1 is closely related to other β-xylosidases from higher plants. The expression of FaXyl1 was strongly associated to the receptacle tissue although a low expression level was detected in achenes and ovaries. The accumulation of FaXyl1 mRNA is ripening-related, starting in white fruit, reaching the maximum at 25–50% red fruit and decreasing thereafter. The total β-xylosidase enzyme activity was detected in all ripening stages with the maximum in 25–50% red fruit. The low activity level detected in immature stages, where no expression of FaXyl1 was found, suggests the presence of other β-xylosidases-like genes. Both the expression of FaXyl1 and the total β-xylosidase activity were down regulated by auxins, as occurs for most of the ripening-related processes in strawberry fruit. A putative role of FaXyl1 and β-xylosidase is discussed." @default.
- W2037834206 created "2016-06-24" @default.
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- W2037834206 date "2004-02-01" @default.
- W2037834206 modified "2023-10-06" @default.
- W2037834206 title "β-xylosidase activity and expression of a β-xylosidase gene during strawberry fruit ripening" @default.
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- W2037834206 doi "https://doi.org/10.1016/j.plaphy.2003.12.001" @default.
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