FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2037839062> ?p ?o ?g. }

• W2037839062 endingPage "15965" @default.
• W2037839062 startingPage "15954" @default.
• W2037839062 abstract "The mechanism of hen egg white lysozyme (HEWL) adsorption on a negatively charged, hydrophilic surface has been studied using atomistic molecular dynamics (MD) simulation. Sixteen 90 ns trajectories provide adequate data to allow a detailed description of the adsorption process to be formulated. Two distinct adsorption sites have been identified. The main one is located on the N,C-terminal protein face and comprises Arg128 (the crucial one), supplemented by Arg125, Arg5, and Lys1; the minor one is used accidentally and contains only Arg68. Adsorption of this protein is driven by electrostatics, where the orientation of the protein dipole moment defines the direction of protein movement. The diffusion range on the surface depends on protein side-chain penetration through the surface water layers. This is facilitated by the long-range electric field of the charged surface, which can align polar side chains to be perpendicular to the surface. A simulation of adsorption onto a neutral ionic surface shows no such surface water layer penetration. Therefore, protein flexibility is seen to be an important factor, and to adsorb the HEWL has to adjust its structure. Nevertheless, at a flat surface only a slight loss of α-helical content is required. The adsorbed HEWL molecule is oriented between side-on and end-on ways, where the angle between the protein long axis (which mostly approximates the dipole moment) and the surface varies between 45° and 90°. Simulations with targeted mutations confirm the picture that emerges from these studies. The active site is located on the opposite face to the main adsorption site; hence, the activity of the immobilized HEWL should not be affected by the surface interactions. Our results provide a detailed insight into the adsorption mechanism and protein mobility at the surface. This knowledge will aid the proper interpretation of experimental results and the design of new experiments and functional systems." @default.
• W2037839062 created "2016-06-24" @default.
• W2037839062 creator A5020577168 @default.
• W2037839062 creator A5020743764 @default.
• W2037839062 date "2010-09-27" @default.
• W2037839062 modified "2023-10-01" @default.
• W2037839062 title "Mechanism of Hen Egg White Lysozyme Adsorption on a Charged Solid Surface" @default.
• W2037839062 cites W122769321 @default.
• W2037839062 cites W1963684165 @default.
• W2037839062 cites W1964858844 @default.
• W2037839062 cites W1965516068 @default.
• W2037839062 cites W1970415994 @default.
• W2037839062 cites W1972136199 @default.
• W2037839062 cites W1972196821 @default.
• W2037839062 cites W1975907747 @default.
• W2037839062 cites W1976338492 @default.
• W2037839062 cites W1976720082 @default.
• W2037839062 cites W1977874572 @default.
• W2037839062 cites W1980040861 @default.
• W2037839062 cites W1985350532 @default.
• W2037839062 cites W1996112440 @default.
• W2037839062 cites W2000790385 @default.
• W2037839062 cites W2000956229 @default.
• W2037839062 cites W2003602307 @default.
• W2037839062 cites W2006174464 @default.
• W2037839062 cites W2009870007 @default.
• W2037839062 cites W2012968377 @default.
• W2037839062 cites W2017381471 @default.
• W2037839062 cites W2021880924 @default.
• W2037839062 cites W2023866898 @default.
• W2037839062 cites W2025559459 @default.
• W2037839062 cites W2029039324 @default.
• W2037839062 cites W2033807814 @default.
• W2037839062 cites W2034912619 @default.
• W2037839062 cites W2037929677 @default.
• W2037839062 cites W2040469701 @default.
• W2037839062 cites W2042661308 @default.
• W2037839062 cites W2043201241 @default.
• W2037839062 cites W2047666357 @default.
• W2037839062 cites W2053845313 @default.
• W2037839062 cites W2059862320 @default.
• W2037839062 cites W2061895862 @default.
• W2037839062 cites W2063744829 @default.
• W2037839062 cites W2064899011 @default.
• W2037839062 cites W2066750660 @default.
• W2037839062 cites W2068002817 @default.
• W2037839062 cites W2074991091 @default.
• W2037839062 cites W2091836140 @default.
• W2037839062 cites W2121545702 @default.
• W2037839062 cites W2150981663 @default.
• W2037839062 doi "https://doi.org/10.1021/la102960m" @default.
• W2037839062 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20873744" @default.
• W2037839062 hasPublicationYear "2010" @default.
• W2037839062 type Work @default.
• W2037839062 sameAs 2037839062 @default.
• W2037839062 citedByCount "99" @default.
• W2037839062 countsByYear W20378390622012 @default.
• W2037839062 countsByYear W20378390622013 @default.
• W2037839062 countsByYear W20378390622014 @default.
• W2037839062 countsByYear W20378390622015 @default.
• W2037839062 countsByYear W20378390622016 @default.
• W2037839062 countsByYear W20378390622017 @default.
• W2037839062 countsByYear W20378390622018 @default.
• W2037839062 countsByYear W20378390622019 @default.
• W2037839062 countsByYear W20378390622020 @default.
• W2037839062 countsByYear W20378390622021 @default.
• W2037839062 countsByYear W20378390622022 @default.
• W2037839062 countsByYear W20378390622023 @default.
• W2037839062 crossrefType "journal-article" @default.
• W2037839062 hasAuthorship W2037839062A5020577168 @default.
• W2037839062 hasAuthorship W2037839062A5020743764 @default.
• W2037839062 hasConcept C117626034 @default.
• W2037839062 hasConcept C127413603 @default.
• W2037839062 hasConcept C147597530 @default.
• W2037839062 hasConcept C147789679 @default.
• W2037839062 hasConcept C150394285 @default.
• W2037839062 hasConcept C159467904 @default.
• W2037839062 hasConcept C173523689 @default.
• W2037839062 hasConcept C178790620 @default.
• W2037839062 hasConcept C185592680 @default.
• W2037839062 hasConcept C204921945 @default.
• W2037839062 hasConcept C2776966407 @default.
• W2037839062 hasConcept C2780358262 @default.
• W2037839062 hasConcept C42475967 @default.
• W2037839062 hasConcept C521977710 @default.
• W2037839062 hasConcept C55493867 @default.
• W2037839062 hasConcept C59593255 @default.
• W2037839062 hasConcept C8010536 @default.
• W2037839062 hasConcept C80107235 @default.
• W2037839062 hasConcept C88001094 @default.
• W2037839062 hasConceptScore W2037839062C117626034 @default.
• W2037839062 hasConceptScore W2037839062C127413603 @default.
• W2037839062 hasConceptScore W2037839062C147597530 @default.
• W2037839062 hasConceptScore W2037839062C147789679 @default.
• W2037839062 hasConceptScore W2037839062C150394285 @default.
• W2037839062 hasConceptScore W2037839062C159467904 @default.
• W2037839062 hasConceptScore W2037839062C173523689 @default.
• W2037839062 hasConceptScore W2037839062C178790620 @default.

• next