FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2037888939> ?p ?o ?g. }

• W2037888939 endingPage "3111" @default.
• W2037888939 startingPage "3104" @default.
• W2037888939 abstract "The present study examined the effect of alterations in GnRH signal pattern (pulsatile vs. continuous; pulse frequency) on mitogen-activated protein kinase (MAPK) activity and whether MAPK plays a role in regulating gonadotrope gene expression. Pituitary MAPK activity was measured by immunoblot, using a phospho-specific MAPK antibody, corrected to the amount of total MAPK per sample. In vivo studies were conducted in adult castrate testosterone-replaced male rats (to suppress endogenous GnRH). Animals received pulsatile or continuous GnRH (or BSA-saline for controls) via jugular cannulas. Initial studies revealed that pulsatile GnRH stimulated a dose-dependent rise in MAPK activity (30 ng, 2-fold increase; 100 ng, 4-fold; 300 ng, 8-fold) 4 min after the pulse. The effect of pulsatile vs. continuous GnRH was examined by administering 50-ng pulses (60-min interval) or a continuous infusion (25 ng/min) for 1, 2, 4, or 8 h. Pulsatile GnRH stimulated a 2- to 4-fold rise in MAPK activity (P < 0.05 vs. controls) that was maintained over the 8-h duration. In contrast, continuous GnRH only increased MAPK activity (2- to 3-fold; P < 0.05 vs. controls) for 2 h, with MAPK activity returning to baseline at later time points. The effect of GnRH pulse frequency on MAPK activation was determined by giving GnRH pulses (50 ng) at 30-, 60-, or 120-min intervals for 8 h. Maximal increases (3-fold vs. controls; P < 0.05) were seen after 120-min pulses, with faster (30- to 60-min interval) pulses stimulating 2-fold increases in MAPK activity (P < 0.05 vs. controls and 120-min GnRH pulse group). The role of MAPK activation on gonadotrope (alpha, LHbeta, FSHbeta, and GnRH receptor) gene expression was determined in vitro. Preliminary studies demonstrated that the MAPK inhibitor, PD-098059 (50 microM), completely blocked GnRH-induced increases in MAPK activity in adult male pituitary cells. Further studies revealed that PD-098059 blocked gonadotrope messenger RNA (mRNA) responses to pulsatile GnRH (100 pg/ml, 60-min interval, 24-h duration) in a selective manner, with alpha, FSHbeta, and GnRH receptor (but not LHbeta) mRNA responses being suppressed. These results show that a pulsatile GnRH signal is required to maintain MAPK activation for durations of longer than 2 h, and that slower frequency pulses are more effective. Further, MAPK plays a crucial role in alpha, FSHbeta, and GnRH receptor mRNA responses to pulsatile GnRH. Thus, divergent MAPK responses to alterations in GnRH signal pattern may be one mechanism involved in differential regulation of gonadotrope gene expression." @default.
• W2037888939 created "2016-06-24" @default.
• W2037888939 creator A5002041109 @default.
• W2037888939 creator A5006555524 @default.
• W2037888939 creator A5062470491 @default.
• W2037888939 creator A5070604004 @default.
• W2037888939 date "1998-07-01" @default.
• W2037888939 modified "2023-09-25" @default.
• W2037888939 title "Gonadotropin-Releasing Hormone Pulses Are Required to Maintain Activation of Mitogen-Activated Protein Kinase: Role in Stimulation of Gonadotrope Gene Expression¹" @default.
• W2037888939 cites W1559986791 @default.
• W2037888939 cites W1578034405 @default.
• W2037888939 cites W1598598013 @default.
• W2037888939 cites W1606575343 @default.
• W2037888939 cites W1968668982 @default.
• W2037888939 cites W1970866361 @default.
• W2037888939 cites W1971334343 @default.
• W2037888939 cites W1975823359 @default.
• W2037888939 cites W1999571693 @default.
• W2037888939 cites W2000082154 @default.
• W2037888939 cites W2005361063 @default.
• W2037888939 cites W2007346285 @default.
• W2037888939 cites W2009038754 @default.
• W2037888939 cites W2010359145 @default.
• W2037888939 cites W2011378156 @default.
• W2037888939 cites W2020791368 @default.
• W2037888939 cites W2023779249 @default.
• W2037888939 cites W2027832713 @default.
• W2037888939 cites W2029334411 @default.
• W2037888939 cites W2033755926 @default.
• W2037888939 cites W2035936653 @default.
• W2037888939 cites W2044025607 @default.
• W2037888939 cites W2047994580 @default.
• W2037888939 cites W2049516453 @default.
• W2037888939 cites W2059011677 @default.
• W2037888939 cites W2059330646 @default.
• W2037888939 cites W2068502127 @default.
• W2037888939 cites W2077563872 @default.
• W2037888939 cites W2079419441 @default.
• W2037888939 cites W2083478963 @default.
• W2037888939 cites W2086524338 @default.
• W2037888939 cites W2106457853 @default.
• W2037888939 cites W2107180907 @default.
• W2037888939 cites W2108415605 @default.
• W2037888939 cites W2125403403 @default.
• W2037888939 cites W2155796235 @default.
• W2037888939 cites W2324816100 @default.
• W2037888939 doi "https://doi.org/10.1210/endo.139.7.6091" @default.
• W2037888939 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9645682" @default.
• W2037888939 hasPublicationYear "1998" @default.
• W2037888939 type Work @default.
• W2037888939 sameAs 2037888939 @default.
• W2037888939 citedByCount "76" @default.
• W2037888939 countsByYear W20378889392012 @default.
• W2037888939 countsByYear W20378889392013 @default.
• W2037888939 countsByYear W20378889392014 @default.
• W2037888939 countsByYear W20378889392016 @default.
• W2037888939 countsByYear W20378889392018 @default.
• W2037888939 countsByYear W20378889392022 @default.
• W2037888939 crossrefType "journal-article" @default.
• W2037888939 hasAuthorship W2037888939A5002041109 @default.
• W2037888939 hasAuthorship W2037888939A5006555524 @default.
• W2037888939 hasAuthorship W2037888939A5062470491 @default.
• W2037888939 hasAuthorship W2037888939A5070604004 @default.
• W2037888939 hasBestOaLocation W20378889391 @default.
• W2037888939 hasConcept C126322002 @default.
• W2037888939 hasConcept C134018914 @default.
• W2037888939 hasConcept C184235292 @default.
• W2037888939 hasConcept C185592680 @default.
• W2037888939 hasConcept C24998067 @default.
• W2037888939 hasConcept C2778575703 @default.
• W2037888939 hasConcept C2778782382 @default.
• W2037888939 hasConcept C2778894405 @default.
• W2037888939 hasConcept C55493867 @default.
• W2037888939 hasConcept C57074206 @default.
• W2037888939 hasConcept C71315377 @default.
• W2037888939 hasConcept C71924100 @default.
• W2037888939 hasConcept C79191276 @default.
• W2037888939 hasConcept C86803240 @default.
• W2037888939 hasConcept C97029542 @default.
• W2037888939 hasConceptScore W2037888939C126322002 @default.
• W2037888939 hasConceptScore W2037888939C134018914 @default.
• W2037888939 hasConceptScore W2037888939C184235292 @default.
• W2037888939 hasConceptScore W2037888939C185592680 @default.
• W2037888939 hasConceptScore W2037888939C24998067 @default.
• W2037888939 hasConceptScore W2037888939C2778575703 @default.
• W2037888939 hasConceptScore W2037888939C2778782382 @default.
• W2037888939 hasConceptScore W2037888939C2778894405 @default.
• W2037888939 hasConceptScore W2037888939C55493867 @default.
• W2037888939 hasConceptScore W2037888939C57074206 @default.
• W2037888939 hasConceptScore W2037888939C71315377 @default.
• W2037888939 hasConceptScore W2037888939C71924100 @default.
• W2037888939 hasConceptScore W2037888939C79191276 @default.
• W2037888939 hasConceptScore W2037888939C86803240 @default.
• W2037888939 hasConceptScore W2037888939C97029542 @default.
• W2037888939 hasIssue "7" @default.
• W2037888939 hasLocation W20378889391 @default.
• W2037888939 hasLocation W20378889392 @default.
• W2037888939 hasOpenAccess W2037888939 @default.

• next