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- W2037902005 abstract "A method was developed to determine the concentration of titanium dioxide in poultry digesta samples, obtained from the lower half of the small intestine of three-week-old male broiler chickens. Titanium dioxide was added at a rate of 5 g kg−1 as a dietary marker. Only 0.1 g of freeze-dried digesta sample was required. The sample was ashed and dissolved in 7.4 M sulphuric acid. Hydrogen peroxide (30% vol.) was subsequently added, resulting in the typical orange colour the intensity of which was dependent upon the titanium concentration. Aliquots of the solutions obtained and of similarly prepared standard solutions were analysed using a UV spectrophotometer and measuring the absorbance at 410 nm. Validation of the method showed that: 1. Prepared samples could be kept for at least 48 h before measuring on the spectrophotometer. 2. Titanium dioxide recovery was not significantly affected by ashing or filtering of the sample during preparation. 3. Other components in the digesta sample did not affect absorbance. 4. Accurate and reproducible results were obtained when using the chicken digesta samples. It was concluded that the method was suitable in the quantitative determination of titanium dioxide concentration in chicken digesta samples obtained during digestibility studies." @default.
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- W2037902005 date "1996-06-01" @default.
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- W2037902005 title "Determination of titanium dioxide added as an inert marker in chicken digestibility studies" @default.
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- W2037902005 doi "https://doi.org/10.1016/0377-8401(95)00916-7" @default.
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