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• W2037978088 abstract "Many biosensors, DNA arrays, and next-generation DNA sequencing technologies need common methods for end modification of random DNA sequences generated from a sample of DNA. Surface immobilization of chemically modified DNA is often the first step in creating appropriate sensing platforms. We describe a simple technique for efficient heterobifunctional modification of arbitrary double-stranded DNA fragments with chosen chemical groups. The modification requires the use of short (10-20 base pairs) synthetic adaptors having desired terminal functional groups and installs known sequences, which can be used for hybridization of primers in the sequencing-by-synthesis approaches. The method, based on ligation under optimized conditions, is selective and provides high yields of the target heterobifunctional DNA product. An additional two-step procedure can be applied to select further for the desired bifunctionalized product using PCR amplification with a chemically modified primer. Both functional groups in the modified DNA are chemically active and can be used in surface immobilization of the DNA strands to create the surface of a biosensor or sequencing chip." @default.
• W2037978088 created "2016-06-24" @default.
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• W2037978088 date "2010-04-28" @default.
• W2037978088 modified "2023-09-24" @default.
• W2037978088 title "Heterobifunctional modification of DNA for conjugation to solid surfaces" @default.
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• W2037978088 doi "https://doi.org/10.1007/s00216-010-3733-5" @default.
• W2037978088 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2888793" @default.
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