FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2037984980> ?p ?o ?g. }

• W2037984980 abstract "Purpose: We have recently described a novel response of human cancer cells to radiation consisting of accumulation of acidic vesicular organelles (AVO) (Cancer Research, 61:439-444, 2001). Acidification of AVO was inhibited by bafilomycin A1 - a specific inhibitor of vacuolar-proton-ATPase. To examine the role of AVO function in the cellular response to radiation, we determined the effect of specific inhibitors of v-H+-ATPase (bafilomycin and concanamycin) on the clonogenic survival of various cancer cell lines. In addition, Northern analysis of mRNA encoding v-H+-ATPase was performed to elucidate possible mechanisms of post-radiation v-H+-ATPase regulation.Materials and Methods: Total RNA was isolated from human cancer cell lines including MCF-7 (breast), LoVo (colon), and LNCaP (prostate) cells 48 hours post-irradiation, and Northern analyses were performed. RNA was separated on a denaturing formaldehyde gel and blotted to a nylon membrane. Probes made from cDNAs to v-H+-ATPase were radioactively labeled, hybridized to the membranes, and exposed to X-ray film for determination of mRNA expression levels. Clonogenic survival assays were performed with MCF-7 and LoVo cell lines. Cells were plated at 30 cells/cm2, and irradiated 24 hours post-plating. Bafilomycin or concanamycin were added to culture media in various concentrations at the time of irradiation. The media was changed after a 24-hour incubation, and colonies were stained with crystal violet for counting 7 days post-irradiation.Results: Radiation-induced accumulation of acidic vesicular organelles in MCF-7, LoVo and LNCaP cells was associated with a two-fold increase in the steady-state level of mRNA for subunit c of v-H+-ATPase. Bafilomycin and concanamycin increased clonogenic cell death after irradiation in a dose-dependent manner. At low concentrations (bafilomycin 2nM, concanamycin 2pM) these agents acted as radiosensitizers, without significant toxicity to unirradiated cells. At 2nM, bafilomycin reduced the clonogenic survival of MCF-7 cells from 0.70 to 0.51 following 2 Gy (p=0.01) and from 0.27 to 0.17 following 4 Gy (p<0.01). Concanamycin (2 pM) reduced the clonogenic survival following exposure to 3 Gy from 0.45 to 0.28 (p=0.015). LoVo cells exhibited a surviving fraction of 0.42 after 3 Gy, versus 0.21 with bafilomycin (p<0.01).Conclusion: These results suggest that formation of acidic vesicular organelles after irradiation serves to protect cells against radiation damage, and that interference with their acidification can decrease clonogenic cell survival post-radiation. Low concentrations of v-H+-ATPase inhibitors such as bafilomycin A1 and concanamycin sensitize cancer cells to radiation without affecting non-irradiated cells. The vacuolar-proton-ATPase can therefore provide a new target for modulation of the radiosensitivity of human epithelial cancer cells." @default.
• W2037984980 created "2016-06-24" @default.
• W2037984980 creator A5007588773 @default.
• W2037984980 creator A5014072244 @default.
• W2037984980 creator A5028805587 @default.
• W2037984980 creator A5069524475 @default.
• W2037984980 creator A5072792566 @default.
• W2037984980 date "2001-11-01" @default.
• W2037984980 modified "2023-10-12" @default.
• W2037984980 title "Vacuolar-proton-ATPase is involved in the response of cancer cells to ionizing radiation and is a new target for radiosensitization" @default.
• W2037984980 doi "https://doi.org/10.1016/s0360-3016(01)02017-x" @default.
• W2037984980 hasPublicationYear "2001" @default.
• W2037984980 type Work @default.
• W2037984980 sameAs 2037984980 @default.
• W2037984980 citedByCount "0" @default.
• W2037984980 crossrefType "journal-article" @default.
• W2037984980 hasAuthorship W2037984980A5007588773 @default.
• W2037984980 hasAuthorship W2037984980A5014072244 @default.
• W2037984980 hasAuthorship W2037984980A5028805587 @default.
• W2037984980 hasAuthorship W2037984980A5069524475 @default.
• W2037984980 hasAuthorship W2037984980A5072792566 @default.
• W2037984980 hasConcept C111337013 @default.
• W2037984980 hasConcept C117262875 @default.
• W2037984980 hasConcept C121332964 @default.
• W2037984980 hasConcept C121608353 @default.
• W2037984980 hasConcept C126322002 @default.
• W2037984980 hasConcept C1491633281 @default.
• W2037984980 hasConcept C153911025 @default.
• W2037984980 hasConcept C181199279 @default.
• W2037984980 hasConcept C18231593 @default.
• W2037984980 hasConcept C185544564 @default.
• W2037984980 hasConcept C190283241 @default.
• W2037984980 hasConcept C191678065 @default.
• W2037984980 hasConcept C203522944 @default.
• W2037984980 hasConcept C23265538 @default.
• W2037984980 hasConcept C2776127477 @default.
• W2037984980 hasConcept C2777722557 @default.
• W2037984980 hasConcept C2779723316 @default.
• W2037984980 hasConcept C54355233 @default.
• W2037984980 hasConcept C55493867 @default.
• W2037984980 hasConcept C71924100 @default.
• W2037984980 hasConcept C81885089 @default.
• W2037984980 hasConcept C86803240 @default.
• W2037984980 hasConcept C96232424 @default.
• W2037984980 hasConceptScore W2037984980C111337013 @default.
• W2037984980 hasConceptScore W2037984980C117262875 @default.
• W2037984980 hasConceptScore W2037984980C121332964 @default.
• W2037984980 hasConceptScore W2037984980C121608353 @default.
• W2037984980 hasConceptScore W2037984980C126322002 @default.
• W2037984980 hasConceptScore W2037984980C1491633281 @default.
• W2037984980 hasConceptScore W2037984980C153911025 @default.
• W2037984980 hasConceptScore W2037984980C181199279 @default.
• W2037984980 hasConceptScore W2037984980C18231593 @default.
• W2037984980 hasConceptScore W2037984980C185544564 @default.
• W2037984980 hasConceptScore W2037984980C190283241 @default.
• W2037984980 hasConceptScore W2037984980C191678065 @default.
• W2037984980 hasConceptScore W2037984980C203522944 @default.
• W2037984980 hasConceptScore W2037984980C23265538 @default.
• W2037984980 hasConceptScore W2037984980C2776127477 @default.
• W2037984980 hasConceptScore W2037984980C2777722557 @default.
• W2037984980 hasConceptScore W2037984980C2779723316 @default.
• W2037984980 hasConceptScore W2037984980C54355233 @default.
• W2037984980 hasConceptScore W2037984980C55493867 @default.
• W2037984980 hasConceptScore W2037984980C71924100 @default.
• W2037984980 hasConceptScore W2037984980C81885089 @default.
• W2037984980 hasConceptScore W2037984980C86803240 @default.
• W2037984980 hasConceptScore W2037984980C96232424 @default.
• W2037984980 hasLocation W20379849801 @default.
• W2037984980 hasOpenAccess W2037984980 @default.
• W2037984980 hasPrimaryLocation W20379849801 @default.
• W2037984980 hasRelatedWork W1592054096 @default.
• W2037984980 hasRelatedWork W1904084051 @default.
• W2037984980 hasRelatedWork W1963591294 @default.
• W2037984980 hasRelatedWork W1973084302 @default.
• W2037984980 hasRelatedWork W2032371550 @default.
• W2037984980 hasRelatedWork W2046011578 @default.
• W2037984980 hasRelatedWork W2046165135 @default.
• W2037984980 hasRelatedWork W2051696890 @default.
• W2037984980 hasRelatedWork W2054764182 @default.
• W2037984980 hasRelatedWork W2057325802 @default.
• W2037984980 hasRelatedWork W2067258157 @default.
• W2037984980 hasRelatedWork W2153484293 @default.
• W2037984980 hasRelatedWork W2158414735 @default.
• W2037984980 hasRelatedWork W2160296373 @default.
• W2037984980 hasRelatedWork W2252567177 @default.
• W2037984980 hasRelatedWork W2354347312 @default.
• W2037984980 hasRelatedWork W2358049382 @default.
• W2037984980 hasRelatedWork W2548296785 @default.
• W2037984980 hasRelatedWork W3030431453 @default.
• W2037984980 hasRelatedWork W3030587034 @default.
• W2037984980 isParatext "false" @default.
• W2037984980 isRetracted "false" @default.
• W2037984980 magId "2037984980" @default.
• W2037984980 workType "article" @default.