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• W2037992568 abstract "We have used a double-labelling flow cytometry analysis of keratin (CK) and DNA in breast cancer. Five monoclonal anti-keratin antibodies were tested: KL1 recognizing Mr 55,000–57,000 keratins, and “anti-glandular epithelia,” LE41, RGE-53, and LP2K specific for CK n° 7, 8, 18, and 19 of Moll's classification, respectively. Flow cytometric (DNA-CK) analysis was performed on 10 benign and 19 malignant human breast tumors. All the benign tumors were diploid and 63% of the malignant tumors were aneuploid. This technique permits the analysis of DNA in the epithelial fraction alone. In aneuploid tumors, gating the DNA-keratin-positive population allowed accurate DNA analysis without interference due to debris background and non-epithelial cells. Moreover, double-labelling using the CK19 antibody gave a better identification of near-diploid tumors. An enhancement of keratin expression in malignant tumors was observed with CK 19 (P < 0.001), KL1 (P < 0.01), CK 8 (P < 0.05), and CK18 (n.s.) compared to benign tumors. The comparison of keratin expression in aneuploid and diploid malignant tumors revealed reduced CK8, CK18, and CK19 in the former." @default.
• W2037992568 created "2016-06-24" @default.
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• W2037992568 date "1990-01-01" @default.
• W2037992568 modified "2023-10-15" @default.
• W2037992568 title "Flow cytometric analysis of DNA content and keratins by using CK7, CK8, CK18, CK19, and KL1 monoclonal antibodies in benign and malignant human breast tumors" @default.
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• W2037992568 doi "https://doi.org/10.1002/cyto.990110609" @default.
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